We narrowed to 25,579 results for: expression vector

1. pCAGIG

   Plasmid

   #11159

   Purpose

   Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   IRES-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNBU2_erm-TetR-P1T_DP-GH023

   Plasmid

   #90324

   Purpose

   Integration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoter

   Depositor

   Andrew Goodman

   Article

   Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-cMYC promoter

   Plasmid

   #114724

   Purpose

   reporter luciferase construct

   Depositor

   Martine Roussel

   Article

   Roussel et al Oncogene. 1994 Feb;9(2):405-15.

   Insert

   C-Myc promoter region
   

   Use

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MS2_GFP

   Plasmid

   #61764

   Purpose

   Expresses MS2 coat protein fused to GFP

   Depositor

   Daniel Larson

   Article

   Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

   Insert

   MS2 coat protein fused to eGFP
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc promoter

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVCT-tTR-KRAB

   Plasmid

   #11643

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   CAG promoters, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCF525-EF1a-Hygro-P2A-mCherry-lenti

   Plasmid

   #115796

   Purpose

   Expresses mCherry in human cells, contained in a lentiviral vector.

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF-1a promoter

   Available since

   Oct. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc

   Plasmid

   #117728

   Purpose

   Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

   Depositor

   Andrew Goodman

   Article

   Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

   Insert

   NanoLuc
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMCSG7-Wt-NmeCas9

   Plasmid

   #71474

   Purpose

   bacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with T7 promoter, N-terminal His tag and TEV site

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

   Insert

   pMCSG7-WT-NmeCas9
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNBU2_erm_P1T_DP-GH023 - NanoLuc

   Plasmid

   #117727

   Purpose

   Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023 upstream of the NanoLuc reporter

   Depositor

   Andrew Goodman

   Article

   Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

   Insert

   NanoLuc
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMA2640

    Plasmid

    #25434

    Purpose

    Retroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin selection

    Depositor

    Mikhail Alexeyev

    Article

    Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

    Insert

    reverse tetracycline-regulated transactivator Advanced
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHIP_RNA1

    Plasmid

    #61259

    Purpose

    Orsay virus RNA1 genome segment expression plasmid for virus reverse genetics

    Depositor

    David Wang

    Article

    Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

    Insert

    Orsay RNA1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    heat shock promoter

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSHDY-Prha-mVenus_rhaS

    Plasmid

    #137662

    Purpose

    Conjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.

    Depositor

    Ilka Axmann

    Article

    Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

    Insert

    Prha:mVenus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    rhaS (Arg218Leu)

    Promoter

    Prha

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHIP_RNA2

    Plasmid

    #61260

    Purpose

    Orsay virus RNA2 genome segment expression plasmid for virus reverse genetics

    Depositor

    David Wang

    Article

    Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

    Insert

    Orsay RNA2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    heat shock promoter

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti

    Plasmid

    #115795

    Purpose

    Expresses AcrIIA4 in human cells, contained in a lentiviral vector.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    AcrIIA4
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1a promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD-6xHis-AviTag-ALFAtag-eGFP

    Plasmid

    #195854

    Purpose

    Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    6xHis-AviTag-ALFAtag-eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVET-tTR-KRAB

    Plasmid

    #11644

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hEF-1alpha, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVPT-tTR-KRAB

    Plasmid

    #11642

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    mPGK, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHRE

    Plasmid

    #134909

    Purpose

    Overexpression of recombinant proteins in plants thanks to efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Test UTR combinations by swapping 5' and 3' UTRs.

    Depositor

    George Lomonossoff

    Article

    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    double 35S

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPiggyBacATCC LacZ GCTT loxAttP

    Plasmid

    #173496

    Purpose

    piggyBac insect transgenesis destination vector for Golden Gate Cloning assemblies

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC18-mini-Tn7T-Gm-GW

    Plasmid

    #64961

    Purpose

    mini-Tn7 Gateway destination vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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