164,826 results

1. pC1-SypHer3s-dmito

   Plasmid

   #108119

   Purpose

   Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

   Insert

   SypHer3s-dmito
   

   Use

   Tags

   ratiometric YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF1-Hs Cdk1/cyclin B1/Cks1

   Plasmid

   #177329

   Purpose

   Co-expression of the human active Cdk1/cyclin B1/Cks1 kinase complex

   Depositor

   Andreas Boland

   Article

   Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

   Inserts

   Cyclin-dependent kinase 1 (CDK1 Human)
   G2/mitotic-specific cyclin-B1 (CCNB1 Human)
   Cyclin-dependent kinases regulatory subunit 1 (CKS1B Human)
   

   Use

   Tags

   2 x Strep-tag and 8 x His

   Expression

   Insect

   Mutation

   Promoter

   p10 and polh

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. BRCA1 A10.2 gRNA

   Plasmid

   #90549

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BRCA1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   BRCA1 (Guide Designation A10.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPROBE-GT

   Plasmid

   #37814

   Depositor

   Steven Lindow

   Article

   Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

   Insert

   Promotorless gfp reporter gene
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

5. SAP145

   Plasmid

   #11239

   Depositor

   Robin Reed

   Article

   Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

   Insert

   SAP145 (SF3B2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 19, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pDEST40-2XHA-Src-K298M

   Plasmid

   #140314

   Purpose

   Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   K298M

   Promoter

   CMV

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCGN N-Ras wt

   Plasmid

   #14723

   Depositor

   Adrienne Cox

   Article

   Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

   Insert

   N-ras (NRAS Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hMLKL-Venus

   Plasmid

   #106078

   Purpose

   expression in mammalian cells

   Depositor

   Douglas Green

   Article

   Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

   Insert

   human MLKL-Venus (MLKL Human)
   

   Use

   Retroviral; Tet-on retrovirus

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Dox-inducible

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-TREX1

   Plasmid

   #27218

   Depositor

   Judy Lieberman

   Article

   Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available Since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pMXs-Nanog

    Plasmid

    #13354

    Purpose

    Retroviral expression of mouse Nanog

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    Nanog homeobox (Nanog Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTagRFP657-C1

    Plasmid

    #31872

    Purpose

    For fusion of TagRFP657 to N-term of insert. SEE DEPOSITOR COMMENTS BELOW.

    Depositor

    Vladislav Verkhusha

    Article

    Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

    Insert

    TagRFP657
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M44Q, K69H, F84W, S148H, S165T, D166A, M167L, L18…

    Promoter

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPathBrick

    Plasmid

    #65006

    Purpose

    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive native promoters

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MCP-EGFP-PiggyBac

    Plasmid

    #190003

    Purpose

    Expression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.

    Depositor

    Tineke Lenstra

    Article

    Brouwer et al Methods Mol Biol. 2024;2694:235-265. doi

    Insert

    MCP-EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CTGF-bio-His

    Plasmid

    #52028

    Purpose

    Expresses full-length Connective tissue growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CTGF (CCN2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBABE puro PPAR delta

    Plasmid

    #8891

    Depositor

    Bruce Spiegelman

    Article

    Brun et al Genes Dev. 1996 Apr 15. 10(8):974-84.

    Insert

    PPAR delta (Ppard Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDC-UAS-nBitbow1.0(miniW)

    Plasmid

    #138329

    Purpose

    Drosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.

    Depositor

    Dawen Cai

    Article

    Veling et al Cell Rep. 2019 Dec 3;29(10):3303-3312.e3

    Inserts

    mAmetrine
    tdKatushka2
    mNeonGreen
    mTFP1
    mKusabira-Orange2
    

    Use

    Tags

    human H2B

    Expression

    Insect

    Mutation

    Promoter

    10xUAS

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-rab9 WT

    Plasmid

    #12663

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB9 (Homo sapiens) (RAB9A Human)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-FLAG-Rheb-N153T

    Plasmid

    #19997

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    Ras homolog enriched in brain 1 - N153T (RHEB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed Asparagine 153 to Threonine

    Promoter

    Available Since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pSpliceExpress

    Plasmid

    #32485

    Depositor

    Stefan Stamm

    Article

    Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX2T E7

    Plasmid

    #13634

    Depositor

    Karl Munger

    Article

    Wu et al J Virol. 1993 Apr . 67(4):2402-7.

    Insert

    HPV 16 E7 (E7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only