We narrowed to 3,422 results for: ttl

1. pdest EGFP Neo

   Plasmid

   #223052

   Purpose

   EGFP gene expression in mammalian cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. dCas9-KRAB

   Plasmid

   #110820

   Purpose

   Conventional dCas9 repressor-dCas9-KRAB

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9-KRAB-MeCP2

   Plasmid

   #110821

   Purpose

   Improved dCas9 repressor-dCas9-KRAB-MeCP2

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmiRA1-MYB

   Plasmid

   #141120

   Purpose

   Lentiviral overexpression of MYB in mammalian cells.

   Depositor

   Jianjun Chen

   Article

   Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

   Insert

   MYB (MYB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-TRE-dCas9-VPR

   Plasmid

   #63800

   Purpose

   SP-dCas9-VPR with doxycycline-inducible expression

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-SpyTag-Flag-SRSF3-V5-mCherry

   Plasmid

   #235091

   Purpose

   SRSF3 mCherry fusion protein without MCP domain

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   pCAG-SpyTag-Flag-SRSF3-V5-mCherry (SRSF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-MCP-SRSF3-V5-mCherry

   Plasmid

   #235090

   Purpose

   SRSF3 mCherry fusion protein with MCP domain

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   pCAG-MCP-SRSF3-V5-mCherry (SRSF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB-CAGGS-dCas9-KRAB

   Plasmid

   #110822

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CAGGS

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL21-mCherry

   Plasmid

   #164410

   Purpose

   Retroviral expression plasmid of sgRNA with mCherry

   Depositor

   Junwei Shi

   Article

   Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for crRNA expression and EFS promoter…

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221-YAP1 CA

    Plasmid

    #79502

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    3XFLAG

    Expression

    Mutation

    S127A

    Promoter

    no Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCHMP2A-NS3-blue

    Plasmid

    #223532

    Purpose

    Mammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.

    Depositor

    Winfried Weissenhorn

    Article

    Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Tags

    NS3 Hepatitis C protease and mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCHMP2A-NS3-Green

    Plasmid

    #223440

    Purpose

    Mammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a green fluorescent protein.

    Depositor

    Winfried Weissenhorn

    Article

    Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Tags

    NS3 Hepatitis C protease and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lentiviral mBmal1-Luc

    Plasmid

    #182762

    Purpose

    Lentiviral vector encoding the promoter region of mouse Bmal1 gene driving a luciferase reporter

    Depositor

    Qing-Jun Meng

    Article

    Gossan et al Arthritis Rheum. 2013 Sep;65(9):2334-45.

    Insert

    mouse arntl promoter (Bmal1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    649 bp

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV_NLS-dSaCas9-NLS-VPR

    Plasmid

    #68495

    Purpose

    AAV vector containing nuclease null SaCas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    dSaCas9
    

    Use

    AAV

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. GABA(A) receptor subunit g2SE

    Plasmid

    #49170

    Purpose

    pHluorin-tagged GABA A receptor subunit (gamma 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

    Depositor

    Tija Jacob, Stephen Moss

    Article

    Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.

    Insert

    GABA(A) receptor subunit gamma 2 (Gabrg2 Mouse)
    

    Use

    Tags

    myc, pHGFP (pHluorin), and thrombin cleavage site…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GABA(A) receptor subunit B3SE

    Plasmid

    #49171

    Purpose

    pHluorin-tagged GABA A receptor subunit (beta 3) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

    Depositor

    Tija Jacob, Stephen Moss

    Article

    Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.

    Insert

    GABA(A) receptor subunit beta 3 (Gabrb3 Mouse)
    

    Use

    Tags

    myc, pHGFP (pHluorin), and thrombin cleavage site…

    Expression

    Mammalian

    Mutation

    GTG (V) to TGC (C)

    Promoter

    CMV

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.CAG.SF-iGluSnFR.A184S

    Plasmid

    #106198

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: A184S

    Promoter

    CAG

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA5-LAP-MPS1-WT

    Plasmid

    #114044

    Purpose

    Expression of exogenous LAP tagged MPS1 WT

    Depositor

    Geert Kops

    Article

    Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

    Insert

    MPS1-WT (TTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT MPS1 sequence

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.CAG.SF-iGluSnFR.A184V

    Plasmid

    #106199

    Purpose

    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184V
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: A184V

    Promoter

    CAG

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pB-CAGGS-dCas9

    Plasmid

    #110823

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge