We narrowed to 83,722 results for: ell
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Plasmid#47778PurposeExpresses enzymatically monobiotinylated partial RH2b ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised RH2b (partial)
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-3'UTR mouse Il13 5xBoxB
Plasmid#207128PurposeTethered luciferase reporter for mouse Il13 BoxBDepositorAvailable SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dCas9-3xNLS-VP64 (Construct 1)
Plasmid#55195PurposeExpresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.DepositorInsertdCas9
UseCRISPR and Synthetic BiologyTagsVP64ExpressionMammalianPromoterCMV/hUBCAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192789PurposeaGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody minimizer
TagsH6 and ybbRExpressionBacterialAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_Std_Dual_epegRNA_tevopreQ1
Plasmid#187454PurposeCoselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmidDepositorInsertATP1A1 G4 Q118R pegRNA (ATP1A1 Human)
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
1072 pBabe puroL myr Akt K179M T308A S473A
Plasmid#9015DepositorInsertAkt1 K179M T308A S473A (AKT1 Human)
UseRetroviralTagsHA and MyrExpressionMammalianMutationK179M T308A S473AAvailable SinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
ICAM5-bio-His
Plasmid#51759PurposeExpresses full-length Intercellular adhesion molecule 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertICAM5 (ICAM5 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPB-3xnls-Tq-Ca-FLITS
Plasmid#145030PurposepiggyBac vector for expressing a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsert3xnls-Tq-Ca-FLITS
Tags3xnls-Tq-Ca-FLITSExpressionMammalianPromoterCMVAvailable SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX304 3xFlag-eRF1 G183/184A_IRES-GFP
Plasmid#198394PurposeeRF1 (AAQ mutant) expressionDepositorInserteRF1 (ETF1 Human)
UseLentiviralTags3xFlagExpressionMammalianMutationmutated GGQ motif (G183/184A)PromoterCMVAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR
Plasmid#160046PurposeFor expressing the full paradoxical circuitDepositorInsertsosTIR1
iaaH
iCasp9
BlastR
UseSynthetic BiologyTagsmAID, mCherry, and mGFPmut3ExpressionMammalianPromoterno promoter, T2A is used and pEF1sAvailable SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1dn (og codon)
Plasmid#174823PurposeMammalian expression of human MLH1dn (original codon usage)DepositorInserthuman MLH1dn (original codon usage) (MLH1 Human)
ExpressionMammalianMutationdeletion of residues 754-756PromoterEF1aAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-EGFP-P2A-mCherry
Plasmid#203868PurposeTet-inducible lentiviral plasmid expressing EGFP-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertEGFP
ExpressionMammalianAvailable SinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiDP1.3
Plasmid#171096Purposelentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Cdt1 (AKA ROLECCS G1) and miniAID-fused mCherry2DepositorInsertOsTIR1-mEmerald-Cdt1-P2A-miniAID-mCherry2
UseLentiviral and Synthetic BiologyTagsEmerald, cdt1, miniAID, mCherry2ExpressionMammalianPromoterCMVAvailable SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mStayGold-TUBA1B
Plasmid#227321PurposeDonor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)DepositorInsertTUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX-TIR-GFP
Plasmid#52737PurposeRetroviral vector expresses the lipid binding domain of p40-phox fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFP and p40 Phox lipid binding domainExpressionMammalianMutationDeleted amino acids 1-85Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
PLC-TIR-GFP
Plasmid#52736PurposeRetroviral vector expresses the lipid binding domain of PLCd1 fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFP and PLCd1 Lipid bindingExpressionMammalianMutationDeleted amino acids 1-85Available SinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
LARG mCherry sspB in pcDNA3.1
Plasmid#90459PurposeRhoA mediated cell migrationDepositorInsertsExpressionMammalianMutationR73QPromoterCMVAvailable SinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
SLP2a-TIR-GFP
Plasmid#52738PurposeRetroviral vector expresses the lipid binding domain of SLP2a fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsC2A domain of Murine SLP2a and GFPExpressionMammalianMutationDeleted amino acids 1-85Available SinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiDP1.2
Plasmid#171095Purposelentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2DepositorInsertOsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
UseLentiviral and Synthetic BiologyTagsEmerald, Geminin, miniAID, mCherry2ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Puro-EF-1α>TurboRFP(ns):T2A:{H2B-RFP}
Plasmid#201120PurposeRFP expression in cells under EF-1α promoter; Histone-bound, nuclearDepositorInsertTurboRFP; H2B-RFP
UseLentiviralMutationNoAvailable SinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only