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pmScarlet_NES_C1

Plasmid

#85060

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NES

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pACYC-Skp-TF

Plasmid

#83921

Purpose

Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Publication

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Trigger Factor

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAG303-Gal-PR50

Plasmid

#84905

Purpose

induce expression of dipeptide repeat PR in yeast

Depositor

Aaron Gitler

Publication

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

PR50 (C9orf72 Synthetic)

Tags

FLAG and Myc

Expression

Yeast

Promoter

GAL1

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGWB540

Plasmid

#74874

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-1xHA CARM1 WT

Plasmid

#81118

Purpose

mammalian expression of CARM1 WT

Depositor

Sung Hee Baek

Publication

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CARM1 (Carm1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pCRISPaint-HaloTag-PuroR

Plasmid

#80960

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HaloTag

Use

Gene tagging

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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NowGFP /pQE-30

Plasmid

#74749

Purpose

NowGFP gene for bacterial expression

Depositor

Konstantin Lukyanov

Publication

Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

Insert

NowGFP

Tags

HisTag (with a mutation but still working)

Expression

Bacterial

Mutation

WasCFP with substitutions E6K, L42M, T43S, V68M, …

Promoter

T5

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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piRFP-N3-Tyrosinase

Plasmid

#80152

Purpose

Melanosome marker

Depositor

Santiago Di Pietro

Publication

Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tyrosinase (TYR Human)

Tags

iRFP682

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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CRY2(535, L348F)-mCherry

Plasmid

#75372

Purpose

Expresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherry

Depositor

Chandra Tucker

Publication

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRY2 residues 1-535 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

L348F

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-nCas9(D10A)-PmCDA1

Plasmid

#79617

Purpose

Expresses nCas9(D10A)-PmCDA1 in yeast cells

Depositor

Akihiko Kondo

Publication

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

PmCDA1

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA5-FRT-TO-EGFP-AID

Plasmid

#80075

Purpose

Mammalian expression vector for N-terminal EGFP-AID tagging

Depositor

Andrew Holland

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP-AID

Tags

AID and EGFP

Expression

Mammalian

Promoter

CMV

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTag-BFP-C-h-Rab7L1-c-Myc

Plasmid

#79803

Purpose

Express TagBFP labeled human Rab7L1.

Depositor

James Johnson

Publication

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab7L1 (RAB29 Human)

Tags

TagBFP and myc

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pLifeAct-miRFP703

Plasmid

#79993

Purpose

F-Actin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lifeact

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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ActTS-GR

Plasmid

#79774

Purpose

FRET-based actinin tension sensors

Depositor

Tetsuya Kitaguchi

Publication

Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ActTS-GR

Expression

Mammalian

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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OPA1-isoform1

Plasmid

#70173

Purpose

Mammalian expression of hsOPA1 isoform 1

Depositor

David Chan

Publication

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 1 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-V1-ORF

Plasmid

#73635

Purpose

Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 1 (V1).

Depositor

Darren Saunders

Publication

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pUCP20T-eyfp

Plasmid

#78466

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Publication

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

eYFP

Expression

Bacterial

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPB-CAG-rtTA-IN

Plasmid

#60612

Purpose

Backbone for tet-inducible genes

Depositor

Austin Smith

Publication

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Type

Empty backbone

Available since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pdCas9-DNMT3A-PuroR (ANV)_v2

Plasmid

#74408

Purpose

Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA

Depositor

Vlatka Zoldos

Publication

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A S. pyogenes, Human, Synthetic)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9; E756A inactiva…

Promoter

CBh

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pTC217

Plasmid

#70018

Purpose

Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

Depositor

Daniel Voytas

Publication

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (Cas9/sgRNA) + Donor + GVR

Use

CRISPR

Expression

Plant

Available since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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