165,744 results

1. mCherry KNL1 wt hardened

   Plasmid

   #45224

   Depositor

   Michael Lampson, Iain Cheeseman

   Article

   Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

   Insert

   mCherry KNL1 resistant to KNL1 siRNA (KNL1 Human)
   

   Tags

   TEV S15

   Expression

   Mammalian

   Available Since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

2. GFP-Foxo1

   Plasmid

   #17551

   Depositor

   Domenico Accili

   Article

   Frescas et al J Biol Chem. 2005 May 27. 280(21):20589-

   Insert

   pEGFP-N1 Foxo1 (Foxo1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. 8xNFAT-ZsG-hCD4

   Plasmid

   #162746

   Purpose

   NFAT-driven ZsGreen-1 reporter gene with human CD4 gene

   Depositor

   Maki Nakayama

   Article

   Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

   Insert

   8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-GAL4

   Plasmid

   #24345

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   GAL4
   

   Expression

   Mammalian

   Available Since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINF

   Plasmid

   #26042

   Depositor

   Ray Owens

   Article

   Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

   Insert

   His3C-lacZ
   

   Tags

   MAHHHHHHSSGLEVL

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUCDM

   Plasmid

   #110742

   Purpose

   Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

   Depositor

   Tim Richmond

   Article

   Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

   Type

   Empty backbone

   Expression

   Insect

   Promoter

   Polyhedrin and p10 promoters

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3/Flag-METTL14

   Plasmid

   #53740

   Purpose

   For expression of METTL14 (methyltransferase-like 14) in human cells

   Depositor

   Chuan He

   Article

   Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

   Insert

   Methyltransferase-like 14 (METTL14 Human)
   

   Tags

   FLAG tag

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti-25xAARE-minP-EGFP

   Plasmid

   #159666

   Purpose

   EGFP reporter plasmid containing 25 tandem repeats of the amino acid response element (AARE)

   Depositor

   Seiichi Oyadomari

   Article

   Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

   Insert

   25xAARE-minP-EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Human SERCA2b (pcDNA3.1+)

   Plasmid

   #75188

   Purpose

   Mammalian expression of ATP2A2

   Depositor

   David MacLennan, Jonathan Lytton

   Article

   Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

   Insert

   ATP2A2 (ATP2A2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdCas9-sgRNA-RFP

    Plasmid

    #166005

    Purpose

    Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

    Depositor

    Martin Buck

    Article

    Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-flag S127A YAP

    Plasmid

    #27370

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    S127A ( major phosphorylation site in YAP2)

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

    Plasmid

    #128424

    Purpose

    Human Codon Optimized dCas9-DNMT3A3L

    Depositor

    Bradley Bernstein

    Article

    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

    Insert

    dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    T7 Promoter

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. 8xNFAT-ZsG-mCD8

    Plasmid

    #162747

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCyCBE

    Plasmid

    #226505

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in cyanobacteria

    Depositor

    Weizhong Chen

    Article

    Li et al Commun Biol. 2024 Aug 14;7(1):994. doi:

    Inserts

    APOBEC1-Cas9D10A
    sgRNA
    sacB gene
    

    Use

    CRISPR

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody

    Plasmid

    #138132

    Purpose

    Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    EGFP-anti-mouse-IgG(kappa)-nanobody
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221-NPC1L1_STOP

    Plasmid

    #161469

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    NPC1L1 (NPC1L1 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcDNA Flag ERbeta

    Plasmid

    #35562

    Depositor

    Harish Srinivas

    Article

    Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

    Insert

    Estrogen Receptor beta (ESR2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSP3545

    Plasmid

    #46888

    Purpose

    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

    Depositor

    Gary Dunny

    Article

    Bryan et al Plasmid. 2000 Sep;44(2):183-90.

    Type

    Empty backbone

    Use

    Nisin-inducible expression; gram-positive bacteri…

    Expression

    Bacterial

    Promoter

    PnisA

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. miniCMV-tdTomato-tDeg

    Plasmid

    #217521

    Purpose

    Expresses tdTomato-tDeg fluorogenic protein in mammalian cells with miniCMV promoter

    Depositor

    Xing Li

    Article

    Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

    Insert

    tdTomato-tDeg fluorogenic protein
    

    Expression

    Mammalian

    Promoter

    miniCMV

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. gRNA-MS2x2-TagRFP657

    Plasmid

    #184441

    Purpose

    sgRNA with MS2 aptamer, TagRFP657 as selection marker

    Depositor

    Rhys Allan

    Article

    Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

    Insert

    sgRNA-MS2x2, TagRFP657
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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