We narrowed to 59,831 results for: Gene

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Oct4-IRES-eGFP-PGK-Neo

Plasmid

#48681

Purpose

For knock-in donor vector by CRISPR/CAS system

Depositor

Rudolf Jaenisch

Article

Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

Insert

Oct4 (Pou5f1 Mouse)

Use

CRISPR

Tags

GFP

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDonor_sU6

Plasmid

#69351

Purpose

sgRNA scaffold and synthetic U6 promoter

Depositor

Andrea Ventura

Article

Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

Insert

sU6 promoter and sgRNA scaffold flanked by BbsI sites

Use

CRISPR

Promoter

synthetic U6 promoter

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flag-hSox2

Plasmid

#20073

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

hSox2 (SOX2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FLEX-tdTomato-WPRE

Plasmid

#51503

Purpose

Can be used to generate AAV virus that will express tdTomato in the presence of Cre

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdTomato

Use

AAV

Promoter

pCAG

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1-P2A-ERG

Plasmid

#97045

Purpose

Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-GfaABC1D-Cre-4x6T

Plasmid

#196410

Purpose

Astrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Has Service

AAV5

Insert

Cre

Use

AAV; Astrocyte-selective

Expression

Mammalian

Promoter

CAG and GfaABC1D

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_DA1h

Plasmid

#113050

Purpose

express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neurons

Depositor

Yulong Li

Article

Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

Has Service

AAV Retrograde and AAV9

Insert

GPCR activation based DA sensor GRAB_DA1h (DRD2 Human)

Use

AAV

Promoter

hSyn

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-p48

Plasmid

#11616

Depositor

Steven Johnson

Insert

Interferon-Stimulated Gene Factor 3 (IRF9 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
pGP-AAV-syn-FLEX-jGCaMP8f-WPRE

Plasmid

#162379

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8f

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.AX

Plasmid

#195218

Purpose

AAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)

Depositor

Viviana Gradinaru

Article

Jang et al Nat Biotechnol. 2023 Jan 26. doi: 10.103

Insert

Synthetic construct isolate AAV-PHP.AX VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_DA1m

Plasmid

#113049

Purpose

express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neurons

Depositor

Yulong Li

Article

Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

Has Service

AAV9

Insert

GPCR activation based DA sensor GRAB_DA1m (DRD2 Human)

Use

AAV

Promoter

hSyn

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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AAV pmSyn1-EBFP-Cre

Plasmid

#51507

Purpose

Can be used to generate AAV virus that will express Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Has Service

AAV5

Insert

EBFP-Cre

Use

AAV

Promoter

pmSyn1

Available Since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-p48

Plasmid

#11614

Depositor

Steven Johnson

Insert

Interferon-Stimulated Gene Factor 3 (IRF9 Human)

Tags

HA

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
pJK-caCas9-NatMX-Neut5L-Leu2 drive

Plasmid

#89578

Purpose

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

Depositor

George Church

Article

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Insert

Leu2 gene drive

Expression

Yeast

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-mCherry

Plasmid

#114470

Purpose

mCherry Control

Depositor

Karl Deisseroth

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

mCherry

Use

AAV

Promoter

Ef1a

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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HSC1-HS4-GiP

Plasmid

#58540

Purpose

Derived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR

Depositor

James Ellis

Article

Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

Inserts

Enhanced Green Fluorescence Protein, Puromycin resistance gene
Core HS4 insulator sequence (dimer)

Use

Retroviral

Expression

Mammalian

Promoter

Human EF1-alpha

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA

Plasmid

#154867

Purpose

Flp-dependent hM4D(Gi) DREADD-mCherry

Depositor

Ulrik Gether

Article

Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03

Has Service

AAV Retrograde and AAV8

Insert

hM4D(Gi)-mCherry

Use

AAV; Flp-frt

Tags

mCherry

Expression

Mammalian

Promoter

Human Synapsin-1

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_DA2h

Plasmid

#140554

Purpose

Expresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neurons

Depositor

Yulong Li

Article

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has Service

AAV9

Insert

The 2nd generation DA sensor GRAB_DA2h (high affinity)

Use

AAV

Promoter

hSyn

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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psi-CHECK3-3xBoxB-MS2

Plasmid

#136011

Purpose

3 repeats of BoxB and MS2 hairpins inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid for the Tethering assay

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

No gene (Artificial)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-mApg5(K130R)

Plasmid

#22959

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

130 Lysine to Arginine

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pRF0G-Tyr

Plasmid

#113644

Purpose

Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.

Depositor

Jeffrey Barrick

Article

Monk et al ACS Synth Biol. 2016 Sep 20.

Insert

MjYRS and tRNA gene

Use

Synthetic Biology

Available Since

July 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry

Plasmid

#35512

Purpose

AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV2

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123T and T159C

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_NE1h

Plasmid

#123309

Purpose

Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neurons

Depositor

Yulong Li

Article

Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

Has Service

AAV Retrograde and AAV9

Insert

GPCR activation based NE sensor GRAB_NE1h

Use

AAV

Promoter

hSyn

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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LB901: pMVP/PB/Blast-DEST

Plasmid

#121873

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LC201: pMVP/PB/Puro-DEST

Plasmid

#121874

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChR2(H134R)-GFP

Plasmid

#58880

Purpose

AAV expression of ChR2(H134R)-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Has Service

AAV Retrograde and AAV8

Insert

ChR2(H134R)-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

H134R

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

Plasmid

#35509

Purpose

AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV5 and AAV9

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T and T159C

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE

Plasmid

#162380

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8s

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

CAG

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

Plasmid

#162377

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8s

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

Plasmid

#125712

Purpose

Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Article

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

eOPN3

Use

AAV

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

short version of αCamKinase II promoter (0.4kb)

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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