We narrowed to 14,584 results for: pET

1. pETM6-H10-mCherry

   Plasmid

   #66531

   Purpose

   Expresses mCherry under the control of Mutant T7 Promoter H10

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon Optimized for E. coli

   Promoter

   Mutant T7 Promoter - H10

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28_6xHis-PARP1-A762V

   Plasmid

   #174795

   Purpose

   Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A762V (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A762V

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBP-pET-RBS

   Plasmid

   #72981

   Purpose

   PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   pET RBS derived from pET vectors
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETM6-3F2-mCherry

   Plasmid

   #73418

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 3F2 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P3F2 (orthogonal T7-lac variant)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-PLS-KP2_rpoA-U

   Plasmid

   #190956

   Purpose

   Test the U-to-C editing activity of KP2 on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-PLS-KP3_rpoA-U

   Plasmid

   #190960

   Purpose

   Test the U-to-C editing activity of KP3 on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP3
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET12a-KIo18/08 C214S

   Plasmid

   #47099

   Depositor

   Marina Ramirez-Alvarado

   Insert

   KIo18/08 C214S (IGKV3-20 Human)
   

   Use

   Tags

   ompT

   Expression

   Bacterial

   Mutation

   C214S

   Promoter

   T7

   Available since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pET28a-PKM2-H391Y

   Plasmid

   #122692

   Purpose

   Express His-tagged PKM2 (H391Y) protein in bacteria.

   Depositor

   Wen-Ching Wang

   Article

   Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

   Insert

   Pyruvate kinase M2 isoform (PKM Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   H391Y

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28b-trAtCCD8(pYL736)

   Plasmid

   #178287

   Purpose

   Expresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   trAtCCD8
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETCON-IgG1/design_50

    Plasmid

    #30301

    Depositor

    David Baker

    Article

    Fleishman et al J Mol Biol. 2011 Sep 29.

    Insert

    IgG Fc Designed Binder IgG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 8, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pET28a His6Flag2AUF1(p42)

    Plasmid

    #135941

    Purpose

    Expresses AUF1p42 in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Use

    Tags

    Flag2 and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSHDY*in_PpetE:BCD2:mVenus

    Plasmid

    #133970

    Purpose

    Heterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.

    Depositor

    Pia Lindberg

    Article

    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

    Insert

    mVenus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    inserted gene is codon optimized for Synechocyst…

    Promoter

    PpetE:BCD2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-SpyTag002 DA-MBP

    Plasmid

    #102832

    Purpose

    Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyTag002 DA-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Reactive aspartic acid has been mutated to an ala…

    Promoter

    T7

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28-At_PRORP1-His

    Plasmid

    #67869

    Purpose

    bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

    Insert

    PRORP1 (PRORP1 Mustard Weed)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Residues 36-572 (see comments)

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT7CFE1-TelR15-YPet

    Plasmid

    #49646

    Purpose

    TALEYellow-Telomere for In Vitro Translation

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

    Insert

    TelR15
    

    Use

    In vitro translation

    Tags

    YPet

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-28a(+)-HMGS

    Plasmid

    #108945

    Purpose

    heterologous expression of HMGS of E. faecalis in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    HMG-CoA synthase (HMGS)
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    A110G

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-ZZ(1700-1751)

    Plasmid

    #99334

    Purpose

    Bacterial expression of mouse CBP ZZ domain

    Depositor

    Peter Wright

    Article

    Legge et al J Mol Biol. 2004 Oct 29;343(4):1081-93.

    Insert

    CBP ZZ domain (Crebbp Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ymScarletI-YPET pDRF1-GW

    Plasmid

    #168063

    Purpose

    FRET fusion between ymScarletI and YPET for use in yeast

    Depositor

    Bas Teusink

    Insert

    ymScarletI-YPET
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET32a-His-FADD

    Plasmid

    #78803

    Purpose

    To purify His-tagged recombinant FADD protein

    Depositor

    Jaewhan Song

    Insert

    GIG3 (FADD Human)
    

    Use

    Tags

    6x His, S-Tag, Thrombin cleavage site, and TrxA (…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pET-Cdc12-Cdc11

    Plasmid

    #131162

    Purpose

    Expression of Septin rods

    Depositor

    Nils Johnsson

    Article

    Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

    Insert

    6his-Cdc12 + Cdc11
    

    Use

    Bicistronic

    Tags

    Cdc11: S-Tag and Cdc12: 6his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET21-pelB-LaG-2

    Plasmid

    #172739

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a-PTBP1-RRM12CCtoSS

    Plasmid

    #89155

    Purpose

    Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
    

    Use

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-ClbN-NHis

    Plasmid

    #51497

    Purpose

    NRPS module containing CATE domains with N-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbN
    

    Use

    Tags

    6x His tag and T7 tag

    Expression

    Bacterial

    Mutation

    contains aa30-1094 of NP_754347.1

    Promoter

    T7

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-PesaR-Plux

    Plasmid

    #47659

    Purpose

    PesaR-P promoter, luxCDABE, and terminator in pET17b (AmpR)

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Synth Biol. 2013 Jul 23.

    Insert

    PesaR-P
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Additional esa box downstream of the original esa…

    Promoter

    PesaR-P

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pET - FnR062.Y78W

    Plasmid

    #84802

    Purpose

    bacterial expression of FnR.0.6.2-Y78W protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78W
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78W

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

26. pET - FnR062.Y78D

    Plasmid

    #84786

    Purpose

    bacterial expression of FnR.0.6.2-Y78D protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78D
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78D

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

27. pET - FnR062.Y78F

    Plasmid

    #84788

    Purpose

    bacterial expression of FnR.0.6.2-Y78F protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78F
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78F

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

28. pET - FnR062.Y78G

    Plasmid

    #84789

    Purpose

    bacterial expression of FnR.0.6.2-Y78G protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78G
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78G

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

29. pET - FnR062.Y78H

    Plasmid

    #84790

    Purpose

    bacterial expression of FnR.0.6.2-Y78H protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78H
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78H

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

30. pET - FnR062.Y78I

    Plasmid

    #84791

    Purpose

    bacterial expression of FnR.0.6.2-Y78I protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78I
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78I

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

31. pET - FnR062.Y78P

    Plasmid

    #84796

    Purpose

    bacterial expression of FnR.0.6.2-Y78P protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78P
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78P

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

32. pET - FnR062.Y78R

    Plasmid

    #84798

    Purpose

    bacterial expression of FnR.0.6.2-Y78R protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78R
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78R

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

33. pET - FnR062.Y78S

    Plasmid

    #84799

    Purpose

    bacterial expression of FnR.0.6.2-Y78S protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78S
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y78S

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

34. pET - FnR062.Y78

    Plasmid

    #84783

    Purpose

    bacterial expression of FnR.0.6.2-Y78 protein

    Depositor

    Benjamin Hackel

    Article

    Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

    Insert

    FnR.0.6.2-Y78
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

35. pET41s-GST-ATF6a-AD

    Plasmid

    #64774

    Purpose

    bacterial expression of GST-tagged ATF6 (1-150)

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    ATF6a-AD (1-150) (ATF6 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-term deletion

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pET28-p53 (1-73) (72R)

    Plasmid

    #62082

    Purpose

    Expression of human p53 (1-73) (72R) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HIs

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; 72R

    Promoter

    T7

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pET11a-p52(1-398)

    Plasmid

    #44733

    Depositor

    Gourisankar Ghosh

    Insert

    p52 (NFKB2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AA 1-398

    Promoter

    T7

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

38. pET28a-Hook3 aa 1-239

    Plasmid

    #74609

    Purpose

    Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28b+R3(R112C/T180C)

    Plasmid

    #108195

    Purpose

    Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA3(R112C/T180C) fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pETO18G-H7C/R86C

    Plasmid

    #27403

    Depositor

    Jonathan Javitch

    Article

    Zhao et al Nature. 2010 May 13. 465(7295):188-93.

    Insert

    leuT
    

    Use

    Tags

    HisX8 and Thrombin

    Expression

    Bacterial

    Mutation

    Changes His 7 to Cys and Arg 86 to Cys

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge