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Plasmid#171050PurposeDonor template for CRISPR/Cas9 targeting of TERT RE3DepositorInsertPuromycin
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQlox66R
Plasmid#135657PurposeClostridial vector, encoding a targetron carrying a lox66 site in the reverse (antisense) orientation. Retarget intron to insert lox66 onto genome at desired site.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQadd1R
Plasmid#135661PurposeClostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem, in the reverse (antisense) orientation. Retarget intron to insert double-lox site at desired location.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQlox71R
Plasmid#135656PurposeClostridial vector, encoding a targetron carrying a lox71 site in the reverse (antisense) orientation. Retarget intron to insert lox71 onto genome at desired site.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQlox71F
Plasmid#135655PurposeClostridial vector, encoding a targetron carrying a lox71 site in the forward (sense) orientation. Retarget intron to insert lox71 onto genome at desired site.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQlox66F
Plasmid#135658PurposeClostridial vector, encoding a targetron carrying a lox66 site in the forward (sense) orientation. Retarget intron to insert lox66 onto genome at desired site.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW1944_ hU6-PV4-loxP-BbsI-ENTRY-gRNA
Plasmid#87572PurposePV4 gRNA entry vector for CRISPR decoderDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW1943_ hU6-PV3-FRT-loxP-BbsI-ENTRY-gRNA
Plasmid#89056PurposePV3 gRNA entry vector for CRISPR decoderDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW759_pCAG-PV2-lox2272-GFP-BGHpA
Plasmid#87575PurposePV2 for 2-input, 4-output decoder circuit and 3-input, 2-output Full AdderDepositorInsertEGFP
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW758_pCAG-PV1-VloxP-FRT-lox2272-NeoR-BGHpA
Plasmid#87580PurposePV1 for 3-input, 2-output Full AdderDepositorInsertNeomycin Resistance Gene
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 Alk.2 shRNA
Plasmid#59297PurposeshRNA to mouse AlkDepositorInsertAlk.2 shRNA
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 Lmo3.5 shRNA
Plasmid#59295PurposeshRNA to mouse Lmo3DepositorInsertLmo3.5 shRNA
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7 Lmo3.8 shRNA
Plasmid#59294PurposeshRNA to mouse Lmo3DepositorInsertLmo3.8 shRNA
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFloxin-IRES-Ofd1-Myc-S75F
Plasmid#24574DepositorInsertOfd1 (Ofd1 Mouse)
UseCre/Lox and Mouse TargetingTagsMycExpressionMutationserine 75 changed to phenylalanine (codon TCT cha…PromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFloxin-Ofd1-Myc-S437R
Plasmid#24577DepositorInsertOfd1 (Ofd1 Mouse)
UseCre/Lox and Mouse TargetingTagsMycExpressionMutationSerine 437 changed to Arginine (codon AGT to CGT)…PromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-Volton2-ST-WPRE
Plasmid#172907PurposeAAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequenceDepositorHas ServiceAAV1InsertVoltron2-ST
UseAAV and Cre/LoxTagssoma localization targeting sequenceExpressionMammalianMutationA122DPromoterhSynapsin1Available sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE
Plasmid#172908PurposeAAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequenceDepositorHas ServiceAAV1InsertAce2N-4AA-mNeon-ST A122D
UseAAV and Cre/LoxTagssoma localization targeting sequenceExpressionMammalianMutationA122DPromoterhSynapsin1Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE
Plasmid#131730PurposeMADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strainDepositorInsertTagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))
UseCre/Lox and Synthetic Biology; Mosaic analysis fo…TagsTagBFP2-V5 tagExpressionMammalianMutationG12VPromoternone (4x polyA to mitigate episomal expression)Available sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-dAPEX2
Plasmid#117174PurposePeroxidase reporter for multiplexed electron microscopy labelingDepositorInsertdAPEX2
UseAAV and Cre/LoxTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMS178
Plasmid#215678PurposeEmpty repair plasmid for ChrII split hygromycinR landing padDepositorInsert5'HA + MCS + loxP + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxTagsExpressionWormMutation5' ∆HYGR encodes aa1-226PromoterAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS114
Plasmid#215677PurposeEmpty repair plasmid for ChrI split hygromycinR landing padDepositorInsert5'HA + MCS + loxN + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxTagsExpressionWormMutation5' ∆HYGR encodes aa1-226PromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ef1a_Large T-antigen_Ires_Puro
Plasmid#18922DepositorInsertLarge T Antigen (SV40gp6 Simian virus 40)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAS088
Plasmid#211502PurposeAAV genome backbone for AAV-Perturb-seq with direct gRNA capture and nuclei sorting based on eGFP.DepositorTypeEmpty backboneUseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV FLEX Synaptophysin GFP
Plasmid#137188PurposeCre-dependent expression of synaptophysin-GFPDepositorInsertSynaptophysin GFP (Syp Mouse)
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lp-BFPnls-LP-Cherry-T2A-NTR2.0
Plasmid#219785PurposeUsed for generating a transgenic line for inducing NTR2.0 expression in Cre positive cells after conversionDepositorInsertmTagBFP2
UseTags3x SV40 NLSExpressionMutationPromoterAvailable sinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-N2cG-WPRE
Plasmid#218757PurposeProvides AND intersectional (Cre+Flp) expression of N2C glycoproteinDepositorInsertN2cG
UseAAVTagsExpressionMutationSee Depositor CommentsPromoterhSynAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDD287 (mKate2^SEC^3xMyc
Plasmid#70685PurposemKate2^SEC^3xMyc vector with ccdB sites for cloning homology armsDepositorInsertmKate2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xMyc and C. elegans codon-optimized mKate2ExpressionWormMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-hM3Dq-mCherry
Plasmid#166599PurposeEncodes Cre-dependent hM3Dq-mCherry under control of the TREDepositorInserthM3Dq-mCherry
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
flex-ChrimsonR-EYFP-Kv
Plasmid#135319PurposeCre-dependent expression of soma-targeted ChrimsonRDepositorInsertChrimsonR
UseAAVTagsEYFPExpressionMutationPromotersynapsinAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
TL028_AAV_hSyn:HaloTag-GluA1
Plasmid#192520PurposeExpression of AMPA GluA1 with N terminal HaloTag fusionDepositorInsertHaloTag-GluA1
UseAAV and Cre/LoxTagsHaloTagExpressionMutationPromoterhSynAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
LeGO-dKatushka2
Plasmid#85214PurposeLentiviral vector expressing dKatushka2 fluorescent protein driven by SFFV promoterDepositorInsertdKatushka2
UseCre/Lox and LentiviralTagsExpressionMammalianMutationBsrGI site removed at 5' end, BsrGI site ins…PromoterSFFVAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-flex-Positron-ST
Plasmid#129267PurposeCre-dependant expression of soma-localized Positron in neuronsDepositorInsertPositron-ST
UseAAVTagsExpressionMammalianMutationD92N, E199VPromoterhsynAvailable sinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-ChR2-TdTomato
Plasmid#166600PurposeEncodes Cre-dependent ChR2-TdTomato under control of the TREDepositorInsertChR2-TdTomato
UseAAVTagsExpressionMutationH134RPromotertetracycline response elementAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVUHshp53
Plasmid#11653DepositorInsertGFP, shRNA against TP53 (TP53 Human)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
CAG-Flex-TCB
Plasmid#48332PurposeCre-dependant TVA-mCherry fusionDepositorInsertTVA receptor mCherry
UseAAVTagsmCherry fusion to TVA receptorExpressionMammalianMutationPromoterCAGAvailable sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
PL552
Plasmid#68407PurposeDonor empty vector with floxed puromycin resistance gene expression cassetteDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromotermouse PGK promoterAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSicoR (Puro) p65 shRNA1
Plasmid#22507DepositorInsertp65 shRNA1 (Rela Mouse)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-stGtACR1(FusionRed)-W3SL
Plasmid#218761PurposeProvides AND intersectional (Cre+Flp) expression of stGtACR1-FusionRedDepositorInsertstGtACR1
UseAAVTagsFusionRedExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-HM4Di-YFP
Plasmid#209193PurposeCre-dependent expression of Hm4Di fused to YFP; ChemogeneticsDepositorInsertHm4Di
UseAAVTagsYFPExpressionMammalianMutationPromoterCAGAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/1-CAG-Flex-FlpO-WPRE
Plasmid#171690PurposeCre-dependent expression of Flp-recombinaseDepositorInsertFlip-recombinase
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAC-BA-lacZ
Plasmid#13423DepositorInsertBA
UseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUC118-FLIP-FlpE-Puro
Plasmid#84539Purposea mock vector for generating FLIP-FlpE targeting vectorsDepositorInsertFLIP-FlpE - Puro
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEM1 = flp-21::LoxPStopLoxP::npr-1 SL2 GFP
Plasmid#24033DepositorInsertNpr1 (npr-1 Nematode)
UseCre/LoxTagsGFPExpressionWormMutationPromoterAvailable sinceFeb. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLEx-TetOn3G-3xHA-FLEx
Plasmid#133729PurposePlasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase.DepositorInsertTetOn3G-3xHA (floxed & inverted)
UseAAVTags3xHA-tagExpressionMammalianMutationPromoterpCMVAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-eDIO-EYFP
Plasmid#104388PurposePiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.DepositorInsertEnhanced Yellow Fluorescent Protein
UseCre/Lox; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS006
Plasmid#211506PurposeAAV genome backbone with a CROP-seq-like design for indirect capture of gRNA sequencesDepositorTypeEmpty backboneUseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-loxP-GRAB_DA2m-loxP
Plasmid#189751PurposeExcludes sensor expression in Cre-positive neuronsDepositorInsertDopamine sensor GRAB_DA2m
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TetON-CD4
Plasmid#217537PurposeAAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged CD4 receptorDepositorArticleInsertCD4 receptor (CD4 Human)
UseAAVTagsHA epitopeExpressionMammalianMutationPromoterTetOperon (TetO)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-TVA-mCherry
Plasmid#38044DepositorInsertAvian sarcoma and leukemia virus receptor 950 (CD320 quail, Synthetic)
UseAAV and Cre/LoxTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMS158
Plasmid#215679PurposeEmpty repair plasmid for ChrIII split hygromycinR landing padDepositorInsert5'HA + MCS + lox2272 + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxTagsExpressionWormMutation5' ∆HYGR encodes aa1-226PromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only