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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,722 results for: Kars

Showing: 2701 - 2750 of 10722 results

  1. pYFAC-CH1

    Plasmid
    #168977
    Purpose
    AMA1 based expression in filamentous fungi. pYFAC-pyrG carrying PalcA ; unique restriction site: PacI
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in fil…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    168977-plasmid-map-sequence-id-359562

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  9. BC-A1-002

    Plasmid
    #78689
    Purpose
    A strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78689-plasmid-map-sequence-id-171045

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  17. pQP-2460

    Plasmid
    #138973
    Purpose
    Light-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1
    Depositor
    Vladislav Verkhusha
    Article
    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:
    Insert
    NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138973-plasmid-map-sequence-id-291734

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  18. pMOD8A-RGR-r10

    Plasmid
    #74370
    Purpose
    This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.
    Depositor
    Eric Klavins
    Article
    Gander et al bioRxiv 041871
    Insert
    RGR-r10
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74370-plasmid-map-sequence-id-141329

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  27. BC-A1-006

    Plasmid
    #78693
    Purpose
    A weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    78693-plasmid-map-sequence-id-171252

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  30. MSP2266

    Plasmid
    #70705
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70705-plasmid-map-sequence-id-131909

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  35. BC-A1-001

    Plasmid
    #78688
    Purpose
    A strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78688-plasmid-map-sequence-id-171043

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  36. pMOD8A-iRGR-r11

    Plasmid
    #74369
    Purpose
    This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.
    Depositor
    Eric Klavins
    Article
    Gander et al bioRxiv 041871
    Insert
    RGR-r11
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    AHD1
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74369-plasmid-map-sequence-id-141324

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  45. pILGFP3A2 (2)

    Plasmid
    #185852
    Purpose
    Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185852-plasmid-map-sequence-id-370761

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  47. BC-A1-004

    Plasmid
    #78691
    Purpose
    A strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78691-plasmid-map-sequence-id-171048

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  48. BC-A1-003

    Plasmid
    #78690
    Purpose
    A strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78690-plasmid-map-sequence-id-171047

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  50. BC-A1-013

    Plasmid
    #78701
    Purpose
    A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78701-plasmid-map-sequence-id-171058

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Showing: 2701 - 2750 of 10722 results