Addgene

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1kb Zfp521 promoter reporter

Plasmid

#104188

Purpose

luciferase reporter to assay Zfp521 promoter activation

Depositor

Kathryn Hentges

Article

Yu et al Genesis. 2016 Oct;54(10):519-533. doi: 1

Insert

Zfp521 promoter - 1 Kb (Zfp521 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

none

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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MPC11 IgG2b gene

Plasmid

#127248

Purpose

Expresses intact IgG2b gene in mammalian cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

IgG2b gene with exons and alt pAs (Ighg2b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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C513-E07: attB5r-<p10p-polyhedrinp>-attB1r

Plasmid

#162949

Purpose

Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pVRc15_524

Plasmid

#49736

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS15_524

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3

Plasmid

#162918

Purpose

Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

firefly luciferase/green fluorescent protein fusion with upstream IRES sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pVRa32_1122

Plasmid

#49689

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF32_1122

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA-RagC(L91P)

Plasmid

#99647

Purpose

Bacterial expression of RagA-RagC(L91P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

L91P

Promoter

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pVRb18_up1700

Plasmid

#49712

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pecf18_up1700

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pVRb16_3622

Plasmid

#49710

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pecf16_3622

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pDOE11 mas:mTq2 transient

Plasmid

#124248

Purpose

Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected plant cells.

Depositor

Attila Fehér

Article

Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsXRN1_1174-1649_AH

Plasmid

#148903

Purpose

Mammalian Expression of HsXRN1_1174-1649

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

Insert

HsXRN1_1174-1649 (XRN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pVRc15_436

Plasmid

#49735

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS15_436

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRa38_1322

Plasmid

#49698

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF38_1322

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Trib2

Plasmid

#131155

Purpose

Mammalian expression of flag-tagged mouse TRIB2

Depositor

Warren Pear, Karen Keeshan

Article

Keeshan et al Cancer Cell. 2006 Nov;10(5):401-11. doi:

Insert

Trib2 (Trib2 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pVRa27_4265

Plasmid

#49682

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF27_4265

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRb25_up4311

Plasmid

#49717

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pecf25_up4311

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pVRb15_up436

Plasmid

#49709

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pecf15_up436

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pK RagA-RagC(Q120L)

Plasmid

#99701

Purpose

Bacterial expression of RagA-RagC(Q120L)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

Q120L

Promoter

T7

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pVRc35_3582

Plasmid

#49750

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS35_3582

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shMePCE

Plasmid

#113536

Purpose

Retroviral vector designed to knock down MePCE.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shMePCE (MEPCE Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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