We narrowed to 3,314 results for: GCT

1. pEPMY1CB0001

   Plasmid

   #162317

   Purpose

   Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven expression in E. coli. Contains mRFP1 reporter.

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEPQD0CM0030

   Plasmid

   #162312

   Purpose

   Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   C-terminal tag, stop codon
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEPQD0KN0025

   Plasmid

   #162283

   Purpose

   Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for T7-driven E. coli cell-free protein synthesis

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmCherry-U6-GTPBP2

   Plasmid

   #140586

   Purpose

   Expresses a gRNA and mCherry in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   gRNA GTPBP2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

   Plasmid

   #118159

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

   Plasmid

   #118160

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTK371

   Plasmid

   #114693

   Purpose

   sgRNA for DHC's N-terminal locus

   Depositor

   Tomomi Kiyomitsu

   Article

   Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

   Insert

   DHC-N sgRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFSBC30

   Plasmid

   #44794

   Depositor

   Christopher Marx

   Article

   Chubiz et al PLoS One. 2012;7(10):e47959. doi: 10.137

   Insert

   FREQ-Seq Barcode: GCTTAG
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1

   Plasmid

   #159905

   Purpose

   Mutagenesis of Slc32a1

   Depositor

   Larry Zweifel

   Insert

   Slc32a1 (Slc32a1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. miniCMV-(mNeonGreen)4-tDeg

    Plasmid

    #129402

    Purpose

    (mNeonGreen)4-tDeg fluorogenic protein

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    (mNeonGreen)-tDeg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. U6.3>Control.gRNA.f+e

    Plasmid

    #99140

    Purpose

    Control gRNA under the regulation of an optimized chicken specific U6 promoter

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    Control gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Chicken U6.3

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p8266 LentiCRISPR v2 hygro sgNT-1

    Plasmid

    #193977

    Purpose

    Expression of spCas9 and non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GESTALT_pX330-v1

    Plasmid

    #103061

    Purpose

    plasmid px330 with guide targeting GESTALT v1 through V5 constructs

    Depositor

    Jay Shendure

    Article

    McKenna et al Science. 2016 May 26. pii: aaf7907.

    Insert

    integration of Cas9 with guide targeting GESTALT barcodes V1 to V5
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pColE1_sgRNA_pos8_AmpR

    Plasmid

    #119151

    Purpose

    Encodes sgRNA targeting position 8 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 8 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO.1 puro-humanU6-shRNA FASN

    Plasmid

    #82327

    Purpose

    used to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)

    Depositor

    Elizabeth Stoll

    Insert

    shRNA to target Fatty Acid Synthase (FASN) (FASN Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTAKN2_J23119-sgRNA

    Plasmid

    #108552

    Purpose

    Bacterial sgRNA expression vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    2x Streptococcus pyogenes sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p8267 LentiCRISPR v2 hygro sgNT-2

    Plasmid

    #193978

    Purpose

    Expression of spCas9 and non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCHW00019

    Plasmid

    #222448

    Purpose

    Level 0, C terminal tag module (TTCG-GCTT) containing GAL4AD-NLS.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    GAL4AD-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BCJJ125

    Plasmid

    #117515

    Purpose

    Cas9_3 (with intron) Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:AATG:Cas9_3(intron):GCTT:BsaI
    

    Use

    CRISPR

    Tags

    2xFLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ZO-1 shRNA 2072

    Plasmid

    #37207

    Depositor

    James Anderson, Alan Fanning

    Article

    Van Itallie et al Mol Biol Cell. 2009 Sep;20(17):3930-40.

    Insert

    dog ZO-1 shRNA (TJP1 canine)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only