We narrowed to 6,084 results for: nls

1. eA3Ai-Cas9n-UGI-NLS

   Plasmid

   #207165

   Purpose

   Express A3A with an N57G point mutation and an intron in a BE3-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)
   

   Use

   Tags

   Cas9-UGI--NLS

   Expression

   Mammalian

   Mutation

   N57G

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PET-HypaSpCas9-NLS-6xHis

   Plasmid

   #207384

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HypaSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   T7

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PET-B-HeFSpCas9-NLS-6xHis

   Plasmid

   #207392

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PET-HeFSpCas9-NLS-6xHis

   Plasmid

   #207391

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PET-evoSpCas9-NLS-6xHis

   Plasmid

   #207389

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CAGGS-SAFB-NLS-3XFLAG-V5

   Plasmid

   #196089

   Purpose

   Expresses mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   SAFB (Safb Mouse)
   

   Use

   Tags

   C-terminal 3xFLAG and C-terminal V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pENTR1a-jAspSnFR3-mRuby3-NLS

   Plasmid

   #203489

   Purpose

   Nuclear directed jAspSnFR3-mRuby3 in Gateway cloning entry vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   jAspSnFR3-mRuby3-NLS
   

   Use

   Gateway entry vector

   Tags

   Nuclear localization signal and mRuby3

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. iB22 PB-zim3-mycNLS-mApple

   Plasmid

   #204718

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MCS-T2a-GFPnls-ER-Cre-ER

   Plasmid

   #200104

   Purpose

   Vector For Twist

   Depositor

   Prayag Murawala

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Twist3cterm-GFPnls-T2A-ER-Cre-ER

    Plasmid

    #200105

    Depositor

    Prayag Murawala

    Insert

    Twist3:cTer-KI-eGFPnls-T2A-ER-Cre-ER
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. C-SWAT IAA17-6FLAG (no NLS)

    Plasmid

    #193322

    Purpose

    C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    IAA17
    

    Use

    Tags

    6FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-2X(CMV-eBFP2-3XNLS-pA)

    Plasmid

    #195867

    Purpose

    to express nuclear localized eBFP2

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev, 20 Aug 2025,

    Insert

    eBFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Flag-NLS-MaSat ZF-18-mVenusN

    Plasmid

    #191392

    Purpose

    Zinc finger fused to N-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    Flag-NLS-MaSat ZF-18-mVenusN
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV2-UbC-3xNLS-mScarlet-I

    Plasmid

    #191099

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Pantelis Tsoulfas, Murray Blackmore

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-mScarlet-I
    

    Use

    AAV and Synthetic Biology

    Tags

    Three repeats of the nuclear localizzation seque…

    Expression

    Mammalian

    Mutation

    optimized to human codon usage

    Promoter

    UbC

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-C1-3x-NLS-mScarlet-AMPKα2

    Plasmid

    #192452

    Purpose

    mScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
    

    Use

    Tags

    3x Nuclear localization signal (NLS) and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKLV2-EF1a-ArgSen (-)NLS

    Plasmid

    #184689

    Purpose

    Lentiviral transfer plasmid for expression of ArgSen (-)NLS

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    ArgSen (-)NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-EF1a-ArgSen (-)NLS

    Plasmid

    #184698

    Purpose

    Lentiviral transfer plasmid for expression of ArgSen (-)NLS

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    ArgSen (-)NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. nPPARg429-gs10-NLS-VPR

    Plasmid

    #188266

    Purpose

    Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    nPPARg429 (PPARG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENT-L1-Kozak-NLSlacZ-stop-L2

    Plasmid

    #186358

    Purpose

    Entry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    lacZ (lacZ Synthetic, E. coli)
    

    Use

    Expression of nuclear lacz reporter

    Tags

    Nuclear localization signal

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 2xNLS-NLP-cMyc AspCas12a

    Plasmid

    #182120

    Purpose

    pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    2xNLS-NLP-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge