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pMC-TNFalpha

Plasmid

#20210

Depositor

Martin Holcik

Article

Zhao et al Cell Death Differ. 2009 Feb . 16(2):244-

Insert

TNFa 3' UTR (TNF Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pKH3-p190

Plasmid

#15547

Purpose

Expression of HA-tagged p190 RhoGAP

Depositor

Ian Macara

Article

Tatsis et al J Biol Chem. 1998 Dec 18. 273(51):34631-

Insert

p190 GTPase activating protein (Arhgap35 Rat)

Tags

Triple HA1 tag

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
Equalizer-L plasmid

Plasmid

#169732

Purpose

Equalizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCMV-tetO2

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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linker-mCitrine-PIF6-NAT

Plasmid

#51576

Purpose

Tag protein of interest with PIF6 in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PIF6 (PIL2 Mustard Weed)

Tags

linker and mCitrine

Expression

Yeast

Mutation

1-100aa of PIF6

Promoter

none

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (W131G)

Plasmid

#169724

Purpose

Mammalian Expression of mouse SPOP W131G

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

W131G

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-i53-bpA-EF1BFP

Plasmid

#111145

Purpose

Expression of i53 and a BFP reporter in mammalian cells

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Inserts

i53 (mutL Synthetic)
BFP (BFP Synthetic)

Expression

Mammalian

Mutation

human Ubiquitin, contains 8 point mutations

Promoter

CAG promoter and EF1

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-24p3/LCN2-Luc

Plasmid

#25463

Depositor

Sarah Gaffen

Article

Shen et al J Leukoc Biol. 2005 Mar . 77(3):388-99.

Insert

24p3/lipocalin 2 proximal promoter (Lcn2 Mouse)

Use

Luciferase

Mutation

none

Available Since

June 25, 2010

Availability

Academic Institutions and Nonprofits only
pKDsgRNA-rssA

Plasmid

#89957

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

rssA gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-Ago1

Plasmid

#21534

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago1 (AGO1 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
FLAG-Matr3 delZnF1

Plasmid

#32883

Depositor

Yossi Shiloh

Article

Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

Insert

MATR3 Matrin3 (MATR3 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

deleted amino acids 283-322

Promoter

CMV

Available Since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-Matr3 delZnF2

Plasmid

#32884

Depositor

Yossi Shiloh

Article

Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

Insert

MATR3 Matrin3 (MATR3 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

deleted amino acids 793-833

Promoter

CMV

Available Since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/Capsid

Plasmid

#120816

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

Capsid ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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FAB(ER-LD)

Plasmid

#214424

Purpose

Detection of ER-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FRB-V5

Tags

CFAST-Halo and RspAN-FRB-V5

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro

Plasmid

#129537

Purpose

PiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.

Depositor

Hideyuki Okano

Article

Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

Insert

tetR-3xFlag-HA-2A-Puro

Use

Synthetic Biology

Promoter

hPGK promoter

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pET-15b PAI-RBP

Plasmid

#45390

Depositor

Thomas Gelehrter

Article

Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

Insert

PAI-RBP (SERBP1 Human)

Tags

His

Expression

Bacterial

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
Lenti-mApple-6xHp

Plasmid

#217376

Purpose

Lipid droplet localization of 6xHp of Spastin fused to mApple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

mApple-6x Hp NC

Use

Lentiviral

Tags

mApple

Promoter

CMV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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WN10151

Plasmid

#80441

Purpose

Expresses human codon-optimized dead (832A) LbCpf1

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Insert

dead (D832A) LbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

D832A

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV WT SPOP 28-359

Plasmid

#194115

Purpose

Wild type His-SUMO SPOP expression plasmid

Depositor

Tanja Mittag

Article

Thomasen et al Elife. 2023 Mar 1;12:e84147. doi: 10.755

Insert

SPOP (SPOP Human)

Tags

6His-SUMO-TEV

Expression

Bacterial

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pETM33_SIN3A_PAH

Plasmid

#178486

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

SIN3A_PAH (SIN3A Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO Plk4 ∆24-mCherry

Plasmid

#80271

Purpose

mammalian expression plasmid for Plk4 with multi-phospho degron deleted

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

mCherry

Expression

Mammalian

Mutation

deletion of multi-phospho degron (amino acids 282…

Promoter

CMV

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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