We narrowed to 92,212 results for: lias

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pAAV-IRES-hrGFP-mWnt3a

Plasmid

#89771

Purpose

Expresses mouse Wnt3a in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Rodriguez et al J Neurosci. 2014 Jul 30;34(31):10285-97.

Insert

mouse Wnt3a (Wnt3a Mouse)

Use

AAV

Tags

None

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

Plasmid

#139090

Purpose

Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

dSpCas9-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLV.tetO.lmx1a

Plasmid

#234847

Purpose

3rd generation lentiviral vector, expresses Lmx1a under control of TetON promoter

Depositor

Malin Parmar

Article

Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

Insert

Lmx1a (Lmx1a Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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hFLT1

Plasmid

#83435

Purpose

human VEGFR1 with HA and FLAG tag

Depositor

Christie Thomas

Article

Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

Insert

human VEGFR1 (FLT1 Human)

Tags

Flag and Myc

Expression

Mammalian

Mutation

HA-tag was inserted 30 AA downstream of the FLT1 …

Promoter

CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pC1-dmito-oROS-HT

Plasmid

#216418

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

dmito-oROS-HT

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-3xFLAG-His6-LARP1

Plasmid

#233626

Purpose

Expression of Flag- and His-tagged LARP1

Depositor

Shintaro Iwasaki

Article

Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

Insert

LARP1 (LARP1 Human)

Tags

3FLAG-His6

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6g

Plasmid

#207857

Purpose

Mammalian expression of PE6g prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6g

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, E471K, H99R, I632V, H721Y, R654C, D64…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-SaPE2

Plasmid

#174817

Purpose

Mammalian expression of SaCas9 prime editor 2

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

SaPE2

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6f

Plasmid

#207856

Purpose

Mammalian expression of PE6f prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6f

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA eIF4GI (1-1599)

Plasmid

#45640

Depositor

Nahum Sonenberg

Article

Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

Insert

eIF4GI transcript variant 2 (EIF4G1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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CTCF-C CRISPR

Plasmid

#140647

Purpose

CTCF tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CTCF (CTCF Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMarsL274G

Plasmid

#63177

Purpose

This plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter.

Depositor

David Tirrell

Article

Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

Insert

MarsL274G (Mars1 Mouse)

Expression

Mammalian

Mutation

L274G

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pHR CD19-mMegf10 CAR

Plasmid

#113013

Purpose

for production of lentivirus encoding the CD19 CAR-Pmegf10

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD19 CAR-P Megf10 intracellular domain (CD19, Megf10 Human, Synthetic, Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEx-HTNC

Plasmid

#13763

Purpose

For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.

Depositor

Klaus Rajewsky

Article

Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

Insert

His-TAT-NLS-Cre

Use

Baculovirus

Tags

His Tag, NLS, and TAT

Expression

Bacterial

Promoter

p10 promoter

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

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pIDTSmart-CMV-PLRS1

Plasmid

#217365

Purpose

Expression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"

Depositor

Abhishek Chatterjee

Article

Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

Insert

leucyl-tRNA synthetase polyspecific mutant

Expression

Mammalian

Mutation

M40I, T252A, Y499I, Y527A, H529G

Promoter

CMV

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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mARGAna (cassette 1, transient)

Plasmid

#197588

Purpose

Second-generation mammalian acoustic reporter gene derived from Anabaena

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

mARGAna (cassette 1)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV pEF1a-DIO-FLPo-WPRE-hGHpA

Plasmid

#87306

Purpose

An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

Depositor

Li Zhang

Article

Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

Insert

Cre-dependent mouse codon-optimized Flp recombinase.

Use

AAV

Tags

N/A

Expression

Mammalian

Promoter

EF1a

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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RFP-ATF4

Plasmid

#125237

Purpose

ATF4 expression vector

Depositor

Rozita Yarmand

Article

Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

Insert

ATF4 (ATF4 Human)

Tags

RFP

Expression

Mammalian

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pC1-oROS-HT-CaaX

Plasmid

#216417

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT-CaaX

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pVitro2-hygro-N-myc-hVps34/hVps15-C-V5-his

Plasmid

#24055

Depositor

Jonathan Backer

Article

Yan et al Biochem J. 2009 Feb 1. 417(3):747-55.

Insert

PIK3C3 and PIK3R4 (PIK3C3, PIK3R4 Human)

Use

Bicistronic

Tags

Myc (hVps34) and V5/his (hVps15)

Expression

Mammalian

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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