177,697 results
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Plasmid#66312PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPR124 (ADGRA2 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cas9/pTREX-n
Plasmid#68708PurposeExpresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
UseCRISPRTagsFusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …MutationC4239T mutation that eliminates a BamHI restricti…Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYLsgRNA-OsU6a/LacZ
Plasmid#66195Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorPromoterOsU6aAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3 AIF 1-90 mCherry FRB
Plasmid#67530PurposeFor expression of mCherry fused FRB to the mitochondrial innermembraneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsAIF a.a. 1 - 90
mCherry
FRB
TagsAIF 1 - 90, FRB, and mCherryExpressionMammalianMutationT->LPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ii-Str_SBP-EGFP-Ecadherin
Plasmid#65288Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEcadherin
TagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-AKAR4-NES
Plasmid#64727PurposeCytosol-targeted, FRET-based PKA activity reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAKAR4-NES
TagsCerulean, NES, and cpVenus[E172]ExpressionMammalianPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_iSH2-pMagFast2(3x)-iRFP
Plasmid#67298PurposeBlue light dependent photoswitching systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiSH2-pMagFast2(3x)-iRFP (PIK3R1 Synthetic, Human)
ExpressionMammalianPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC18R6K-mini-Tn7T
Plasmid#64958Purposemini-Tn7 base vector with transcriptional terminatorDepositorPublicationTypeEmpty backboneExpressionBacterialAvailable sinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSP1673
Plasmid#65769PurposeBacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNADepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
UseCRISPRTagsNLSExpressionBacterialPromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBa-KIF5C 559-tdTomato-FKBP
Plasmid#64211PurposeKIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tagsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKif5c (Kif5c Rat)
TagsFKBP and tdTomatoExpressionMammalianMutationmotor domain aa1-559Promoterchicken Beta ActinAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-Cre-6xHis
Plasmid#62939PurposeExpression of Cre-6xHis in bacterial cellsDepositorInsertCre recombinase
Tags6xHis tagExpressionBacterialPromoterT7Available sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Mucolipin1-pEGFP C3
Plasmid#62960Purposefull length human Mucolipin-1 cloned into pEGFP C3DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMucolipin-1 (MCOLN1 Human)
TagsEGFPExpressionMammalianMutationMucolipin-1 has 1 silent mutation compared to the…PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSR646
Plasmid#65215PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-6 cap
UseAAVExpressionInsectPromoterP10 and polyhedrinAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E_unc503
Plasmid#64020Purpose5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45bDepositorInsertmuscle-specific promoter of zebrafish unc-45b (unc45b Zebrafish)
UseGateway CloningPromoter503-bp promoter of zebrafish unc-45bAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-BRPF1
Plasmid#65382PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBRPF1 (BRPF1 Human)
TagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Parkin-IRES-zeo
Plasmid#61727PurposeExpression of human Parkin fused to mCherryDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertParkin (PRKN Human)
TagsmCherryExpressionMammalianAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMy-CTCF-GFP
Plasmid#50574PurposeRetroviral vector endoding for mouse CTCF fused to GFPDepositorInsertAmpicilin
UseRetroviralAvailable sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMU-ACTLr
Plasmid#61193PurposeFluorescent reporter for F-actin LifeAct-mCherry expressed under AtUBQ10 promoterDepositorInsertLifeAct-mCherry
TagsmCherryExpressionPlantPromoterAtUBQ10Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBbi-GH
Plasmid#60514PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCWori cytochrome P450-BM3h 9D7
Plasmid#61308PurposeExpresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tagDepositorInsertCytochrome P450-BM3 heme domain 9D7
Tags6xHisExpressionBacterialMutationT268A, I263A, T438V, A328GPromoterTacAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only