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pCAS9-mCherry-Frame +2

Plasmid

#66941

Purpose

CRISPaint frame selector +2

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA frame +2

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti_dCas9-KRAB-MeCP2_Blast

Plasmid

#221501

Purpose

Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

dCas9-KRAB-MeCP2-T2A-Blast

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-ACTG1

Plasmid

#66943

Purpose

CRISPaint target selector ACTG1

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA ACTG1

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-HIST1H4C

Plasmid

#66944

Purpose

CRISPaint target selector HIST1H4C

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA HIST1H4C

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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p3s-Cas9-HF1

Plasmid

#104173

Purpose

Cas9-HF1 mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

Cas9-HF1

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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p3s-eCas9-1.1

Plasmid

#104172

Purpose

eCas9-1.1 mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

eCas9-1.1

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pU6Rosa-CAG-Cas9

Plasmid

#118998

Purpose

Mammalian expression of Rosa26 sgRNA, Cas9 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pdCas9-GFP-p65

Plasmid

#232630

Purpose

p65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacer

Depositor

Karsten Rippe

Insert

dCas9-GFP-p65 (RELA Human, Synthetic)

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-mCherry

Plasmid

#64324

Purpose

Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-mCherry

Expression

Mammalian

Mutation

Promoter

CBh and U6

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-BFP

Plasmid

#64323

Purpose

Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-EBFP2

Expression

Mammalian

Mutation

Promoter

CBh and U6

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

Plasmid

#102888

Purpose

PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo

Plasmid

#102892

Purpose

PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE-tight

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-nEFCas9

Plasmid

#87115

Purpose

SpCas9 expression AAV backbone plasmid under control of nEF promoter

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

nEF-Cas9

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold)

Plasmid

#120296

Purpose

AAV vector; encodes GFP as well as a U6-driven Rosa26-targeting gRNA (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

Rosa26 gRNA (SpCas9 scaffold)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)

Plasmid

#113041

Purpose

AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, CRISPR, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)

Plasmid

#113040

Purpose

AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

gRNA EMX1 (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, CRISPR, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

Plasmid

#113042

Purpose

AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, CRISPR, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA

Plasmid

#86986

Purpose

For cloning and expression of sgRNA together with expression of a Cas9-Venus fusion protein

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9-venus

Use

Tags

Cas9-Venus fusion protein

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only
pUC18-mini-Tn7T-Lac-dCas9 (Ptac)

Plasmid

#105234

Purpose

Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescens

Depositor

Kristala Prather

Article

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus dCas9

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Bacterial

Mutation

D10A, H599A

Promoter

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls

Plasmid

#64113

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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