We narrowed to 2,537 results for: PORA

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pAAV-hSyn-GRAB_g5-HT2h

Plasmid

#208721

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2h in neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2h

Use

AAV

Promoter

hSyn

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCHW110033

Plasmid

#222441

Purpose

Level 0, CDS module (AATG-GCTT) containing Cre recombinase.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

Cre recombinase

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDule-Mb haloTyrRS C6

Plasmid

#160377

Purpose

C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

Depositor

Ryan Mehl

Article

Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

Insert

C6 HaloTyrosine tRNA synthatase

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Mutation

L270S, Y271L, N311G, C313T

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcpAzpaRS_v2

Plasmid

#196483

Purpose

To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.

Depositor

Kensaku Sakamoto

Article

Hino et al Methods Mol Biol. 2012;794:215-28. doi:

Insert

azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA

Expression

Mammalian

Promoter

SV40(synthetase), Pol III tRNA promoter (tRNA), C…

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR

Plasmid

#174883

Purpose

Targeting vector for generating Pou5f1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Pou5f1

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-fDIO-GRAB_eCB2.0

Plasmid

#164610

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

EFS

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCHW110032

Plasmid

#222438

Purpose

Level 0, promoter module (GGAG-AATG) containing CBS4 and CMV1 promoters.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW00011

Plasmid

#222439

Purpose

Level 0, CDSns module (AATG-TTCG) containing S. cerevisiae CUP2.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CUP2 (Saccharomyces cerevisiae) (CUP2 Budding Yeast)

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1alpha-soCoChR-tdTomato

Plasmid

#107714

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-tdTomato

Use

AAV

Tags

KA2 and tdTomato

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_eCB2.0mut

Plasmid

#164607

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

AAV

Mutation

S383T, F177A

Promoter

hSyn

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pTV-Nanog_2-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174882

Purpose

Targeting vector for generating Nanog STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Nanog

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET21-GFP-AURKA

Plasmid

#99875

Purpose

Expression of GFP-AURKA for protein purification in bacteria

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP

Expression

Bacterial

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pTV-Sox2_5prime-1000-24MS96T-3F_NeoR

Plasmid

#174884

Purpose

Targeting vector for generating Sox2 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Sox2

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pscAAV-Tyr

Plasmid

#135616

Purpose

Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus

Depositor

Lin He

Article

Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

Insert

EcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-soCoChR-GFP

Plasmid

#107711

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CaMKII promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Promoter

CaMKII

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBluescript GPX-1

Plasmid

#13038

Depositor

Peter Newburger

Article

Shen et al J Biol Chem. 1993 May 25. 268(15):11463-

Insert

GPX-1 (GPX1 Human)

Expression

Bacterial

Mutation

Additional GCG upstream of codon 11. Codon 92 is …

Available Since

Nov. 7, 2006

Availability

Academic Institutions and Nonprofits only
pUBC_HA_PP7-mVenus

Plasmid

#111268

Purpose

Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag

Depositor

John Rinn

Article

Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

Insert

PP7-mVenus

Use

Synthetic Biology

Tags

HA, SV40 NLS, and mVenus

Expression

Mammalian

Mutation

PP7: C68S, S69G [delta]70-75 (non-aggregating)

Promoter

hUBC

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_OTmut

Plasmid

#185389

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Use

AAV

Promoter

EFS

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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FKBP-alpha(740-977)-GFP

Plasmid

#100731

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2A1 (Ap2a1 Mouse)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCHW110031

Plasmid

#222437

Purpose

Level 0, promoter module (GGAG-CCAT) containing CBS4 and CMV1 promoters.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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