We narrowed to 2,596 results for: ubiquitin

1. pCDNA3.1-Flag-H2A K15R

   Plasmid

   #63567

   Purpose

   Expression of human H2A with K15R tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysine at 15 is now Arginine

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pRGEB-VirD2-Cas9

   Plasmid

   #139803

   Purpose

   Expressing VirD-Cas9 fusion under ubiquitin promoter

   Depositor

   Magdy Mahfouz

   Article

   Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

   Insert

   VirD2-Cas9
   

   Use

   Tags

   VirD2-Cas9

   Expression

   Plant

   Mutation

   Promoter

   Ubiquitin

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA SF-HERC2 F5

   Plasmid

   #55788

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (3550-4450aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (3550-4450aa) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCI Flag Smad3 LC EPSM

   Plasmid

   #27038

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   Smad3 (SMAD3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   aa 133-425; T179V S204A S208A S213A

   Promoter

   Available since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCI Flag Smad3 NL EPSM

   Plasmid

   #27006

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   Smad3 (SMAD3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   aa 1-226; T179V S204A S208A S213A

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

6. px458_2A_GFP_sgRNA_LTN1

   Plasmid

   #127125

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   gRNA LTN1 (LTN1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3flag-RanGAPdeltaN

   Plasmid

   #25104

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   RanGAP1 deleted N-term domain (RANGAP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-203.

   Promoter

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

8. FUG-T2A-Cas9

   Plasmid

   #75346

   Purpose

   Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP (via t2a)

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRK5-Merlin-Flag-HA

   Plasmid

   #27103

   Depositor

   Filippo Giancotti

   Article

   Li et al Cell. 2010 Feb 19. 140(4):477-90.

   Insert

   Merlin (Nf2 Mouse)
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pEGFP-WRAP53beta

    Plasmid

    #64676

    Purpose

    expresses Wild type WRAP53beta

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    WD40-encoding RNA antisense to P53 (WRAP53 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

11. GFP-RNF110

    Plasmid

    #126547

    Purpose

    Expresses GFP-tagged RNF110 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RNF110 (PCGF2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHR RPS27A K113R-3xFlag_IRES-GFP

    Plasmid

    #198391

    Purpose

    RPS27A K113R (ubiquitin/ribosomal protein eS31 fusion) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    ubiquitination site mutant (K113R)

    Promoter

    EF1A

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-parkin 77-465

    Plasmid

    #45970

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin 77-465 (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Dele…

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-parkin 219-465

    Plasmid

    #45972

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin 219-465 (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Dele…

    Promoter

    tac

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS2 Flag Smad3 S204A

    Plasmid

    #27114

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    S204A

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBacPAK8-His-myc-Rbx1

    Plasmid

    #29506

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

    Insert

    Rbx1 (Rbx1 Mouse)
    

    Use

    Tags

    His and myc

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pCS2 Flag Smad3 T179V

    Plasmid

    #26997

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    T179V

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pCMV5 Flag-Par6 delta 250-295 Flag tagged

    Plasmid

    #24646

    Depositor

    Jeff Wrana

    Article

    Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

    Insert

    Par6c (Pard6a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of the PDZ domain of Par6c (aa 250-295)

    Promoter

    Available since

    June 2, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pcDNA3-KLF5-his

    Plasmid

    #40903

    Depositor

    Jin-Tang Dong

    Article

    Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

    Insert

    Kruppel-like factor 5 (KLF5 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-HA-mIFITM3-K24,83,88,104A

    Plasmid

    #58408

    Purpose

    Expresses murine IFITM3-K24,83,88,104A with an N-terminal HA tag in mammalian cells (Lysine-less nonubiquitinated mutant)

    Depositor

    Jacob Yount, Howard Hang

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lysines 24, 83, 88, and 104 to Alanine

    Promoter

    CMV IE

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only