We narrowed to 59,239 results for: %s

1. pMXs-hs-3xHA-delta6xNHL-LIN-41

   Plasmid

   #52722

   Purpose

   retroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tags

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   LIN-41 (TRIM71 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   deleted amino acids 593–868

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJH3456

   Plasmid

   #179581

   Purpose

   Pnmr-1::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 BFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   tomm20::miniSOG (tomm-20 Nematode)
   

   Tags

   BFP

   Expression

   Worm

   Promoter

   Pnmr-1

   Available Since

   March 2, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pSW725 - pUFO>>

   Plasmid

   #116000

   Purpose

   destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pUFO:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp6:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
   

   Tags

   HDEL and SP(ER)

   Expression

   Plant

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUDE710

   Plasmid

   #103020

   Purpose

   expression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)

   Depositor

   Jean-Marc Daran

   Article

   Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

   Insert

   crADE2-3.crHIS4-4.S (ADE2, HIS4 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEThT-RvCAHS12

   Plasmid

   #192468

   Purpose

   Bacterial expression of RvCAHS12

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

   Insert

   CAHS12
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcFLAG-CSK-delta SH3 (73 to450 amino acids)

   Plasmid

   #74504

   Purpose

   Overexpression of CSK delta SH3 in mammalian cells

   Depositor

   Lars Rönnstrand

   Article

   Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

   Insert

   CSK (CSK Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   del-SH3 (73 to450 amino acids)

   Promoter

   CMV

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pUC19-E6MBW6

   Plasmid

   #103102

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
   

   Use

   CRISPR; Cloning vector

   Mutation

   human codon-optimized

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

   Plasmid

   #86611

   Purpose

   Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pInducer10-UBC9-sh4

   Plasmid

   #168988

   Purpose

   Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Ubiquitin Conjugating Enzyme 9
   

   Expression

   Mammalian

   Promoter

   Ubc promoter

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-GFP-2AFP-KDEL

    Plasmid

    #154349

    Purpose

    Mammalian expression of 2AFP

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    2AFP
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. eGFP-KCC2

    Plasmid

    #104075

    Purpose

    expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2

    Depositor

    Igor Medyna

    Article

    Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-9

    Insert

    KCC2 (b isoform) (Slc12a5 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EcoR1 site in rat KCC2 was removed by silent muta…

    Promoter

    CMV

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19.TRIM28.FKBP.knock-in.mCerulean

    Plasmid

    #185009

    Purpose

    For knocking in FKBP F36V P2A mCerulean

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    FKBP F36V P2A mCerulean
    

    Use

    Cloning

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-M4-AR-S81D

    Plasmid

    #171242

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Asp (AR-S81D)

    Depositor

    Steven Balk

    Article

    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

    Insert

    3xFlag-tagged human androgen receptor Ser81Asp mutant (AR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Ser81Asp (AR-S81D)

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1P DDX11_2

    Plasmid

    #160778

    Purpose

    Suppress DDX11

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shDDX11_2
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1P DDX11_1

    Plasmid

    #160777

    Purpose

    Suppress DDX11

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shDDX11_1
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET19-M(BRCT)TdTwt

    Plasmid

    #207459

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    M(BRCT)TdTwt (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSN25

    Plasmid

    #100110

    Purpose

    expresses a chimeric SOX2-repressor fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCitrine-P2A-SOX2 fused to full length HP1 alpha (SOX2, CBX5 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-mNRDc-E>A-V5

    Plasmid

    #51298

    Purpose

    expression of the inactive mutant of mouse NRDc in mammalian cells

    Depositor

    Eiichiro Nishi

    Article

    Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

    Insert

    NRDc E247A (Nrd1 Mouse)
    

    Tags

    HIS and V5

    Expression

    Mammalian

    Mutation

    E247A, enzymatic inactive mutant

    Promoter

    CMV

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCR8/GW/TOPO-SRF

    Plasmid

    #98618

    Purpose

    Gateway entry TOPO TA cloning vector for SRF

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    SRF (Srf Mouse)
    

    Use

    Topo ta cloning vector

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSc-His6-Sem1Sc

    Plasmid

    #83235

    Purpose

    pGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeast

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    Sem 1 (SEM1 Budding Yeast)
    

    Tags

    His6

    Expression

    Yeast

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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