We narrowed to 31,648 results for: REP

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CBLa_1.2_GpA_G83I

Plasmid

#207845

Purpose

BLaTM-System GpA G83I negative control in CBLa 1.2 vector

Depositor

Dieter Langosch

Article

Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

Insert

N-BLa 1.2 fusion protein

Tags

Flag

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCX:γB2ΔICD-EC1-G4S-mTQ2ΔC6

Plasmid

#209089

Purpose

mTurquoise2-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Tags

PA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCX:γB2ΔICD-EC5-VenusΔN3C9-P3

Plasmid

#209090

Purpose

mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Tags

PA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLipoP1-NLS-3xmCitrine

Plasmid

#192522

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

Putative membrane lipoprotein (AT3G18250 Mustard Weed)

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

LipoP1

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-ASAP3ER

Plasmid

#195358

Purpose

ASAP3ER under control of human synapsin promoter

Depositor

Brenda Bloodgood

Article

Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

Insert

ASAP3ER

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Tesmin-BioID2-3xFLAG

Plasmid

#186815

Purpose

CAG promoter-driven expression of TESMIN-BioID2-3xFLAG

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Insert

Tesmin (Tesmin Mouse)

Tags

BioID2-3xFLAG

Expression

Mammalian

Promoter

CAG promoter

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-Luz

Plasmid

#188304

Purpose

Expression of Luz via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

luz

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-LuzGFPHis

Plasmid

#188315

Purpose

Expression of Luz-eGFP via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

Luz-GFP fusion

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-HisGFPLuz

Plasmid

#188314

Purpose

Expression of eGFP-Luz via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

GFP-Luz fusion

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_DUX4_KBM

Plasmid

#187800

Purpose

DUX4 with KBM (p. E414-L424 deletion) with MAC-C tag

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

DUX4-KBM (DUX4 Human)

Expression

Mammalian

Mutation

p. E414-L424 deletion

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_RET

Plasmid

#187775

Purpose

MAC-tagged gene expression of human RET

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

RET (RET Human)

Expression

Mammalian

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3

Plasmid

#73542

Purpose

Expression of sgRNA targeting LINE-1 and TagBFP

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

LINE-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1

Plasmid

#73543

Purpose

Expression of sgRNA targeting LINE-1 and TagBFP

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

LINE-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-mMOV10-3'UTR

Plasmid

#178905

Purpose

dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

3'UTR mMov10 (Mov10 Mouse)

Use

Luciferase

Promoter

SV40 promoter

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-T11HA-hMOV10-CD

Plasmid

#178908

Purpose

expression of catalytic dead hMOV10 K530A

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

cDNA Catalytic dead hMOV10 (MOV10 Human)

Tags

T11 beta strand of GFP-HA

Expression

Mammalian

Mutation

K530A catalytic dead AAG>GCA

Promoter

CMV protmoter

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/TARG1

Plasmid

#172580

Purpose

For generation of FlpIn lines with untagged TARG1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Tags

none

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pSP64T-ythdf1-3xflag

Plasmid

#164488

Purpose

Expresses zebrafish ythdf1 with 3x flag tag, for mRNA injection

Depositor

Antonio Giraldez

Article

Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

Insert

ythdf1 (ythdf1 Zebrafish)

Use

Production of mrna for zebrafish injection.

Tags

3x-flagtag

Promoter

SP6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pColE1-AlphaOmega

Plasmid

#165867

Purpose

Terminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBlastR-GMRWhite

Plasmid

#165885

Purpose

Blasticidin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Transgenesis Ready Selection Vector

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31

Plasmid

#165888

Purpose

Puromycin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

All-in-one FC31 RMCE Cassette

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31

Plasmid

#165889

Purpose

Blasticidin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

All-in-one FC31 RMCE Cassette

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGal4BD 1-147

Plasmid

#165819

Purpose

1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Gal4BD 1-147

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGAL4AD 768-881

Plasmid

#165820

Purpose

768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GAL4AD 768-881

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Bax gRNA#1

Plasmid

#171827

Purpose

Cas9-mediated knockout of Bax in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bax (Bax Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Bax gRNA#2

Plasmid

#171828

Purpose

Cas9-mediated knockout of Bax in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bax (Bax Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Bax gRNA#3

Plasmid

#171829

Purpose

Cas9-mediated knockout of Bax in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bax (Bax Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Ssh2 gRNA#2

Plasmid

#163398

Purpose

Cas9-mediated knockout of Ssh2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh2 (Ssh2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Ssh2 gRNA#1

Plasmid

#163397

Purpose

Cas9-mediated knockout of Ssh2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh2 (Ssh2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Ssh2 gRNA#3

Plasmid

#163399

Purpose

Cas9-mediated knockout of Ssh2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh2 (Ssh2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Ssh3 gRNA#2

Plasmid

#163401

Purpose

Cas9-mediated knockout of Ssh3 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh3 (Ssh3 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Ssh3 gRNA#3

Plasmid

#163402

Purpose

Cas9-mediated knockout of Ssh3 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh3 (Ssh3 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAFNF-Cdc42

Plasmid

#163606

Purpose

Expresses Cdc42 Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Cdc42 (Cdc42 Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Ssh3 gRNA#1

Plasmid

#163400

Purpose

Cas9-mediated knockout of Ssh3 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh3 (Ssh3 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTub-alpha-1-mtGFP

Plasmid

#138981

Purpose

Neuron-specific expression of membrane targeted EGFP

Depositor

Frank Bradke, Sebastián Dupraz

Article

Santos et al Cell Rep. 2020 Jul 21;32(3):107907. doi:

Insert

EGFP

Expression

Mammalian

Mutation

EGFP (added membrane targeting domain from GAP43).

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11a-Z-N-DiB2

Plasmid

#168473

Purpose

N-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip protein

Depositor

Jens Meiler

Article

Bozhanova et al Sci Rep. 2020 Jul 6;10(1):11049. doi: 10

Insert

N-fragment of DiB2 + Leucine Zipper

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGMR-WhiteA2

Plasmid

#165874

Purpose

Basic transgenesis 5xGMR white CDS physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJF144_SECGFP_sg2

Plasmid

#163865

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJF152_Phsp_Cas9

Plasmid

#163862

Purpose

heat shock-induced expression of Cas9 in C. elegans

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

Cas9, C. elegans codon optimized with intron and SV40_NLS

Use

CRISPR

Expression

Worm

Promoter

Phsp-16.48

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJJF143_SECGFP_sg1

Plasmid

#163864

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPuroR-GMRWhite

Plasmid

#165884

Purpose

Puromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Transgenesis Ready Selection Vector

Use

Synthetic Biology

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-OMACS

Plasmid

#136765

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Acsm4

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Acsm4 (Acsm4 Mouse)

Use

In situ probe

Mutation

ISH Probe insert, A102T- please see depositor com…

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC_h7SK M15 (CUA)

Plasmid

#154784

Purpose

plasmid with single copy of Mma PylT M15 mutant (CUA anticodon)

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

Mma PylT

Expression

Mammalian

Mutation

G10A, G14U, G16U, C20U, C52A

Promoter

h7SK

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC_h7SK M15 (UUA)

Plasmid

#154785

Purpose

plasmid with single copy of Mma PylT M15 mutant (UUA anticodon)

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

Mma PylT

Expression

Mammalian

Mutation

UUA anticodon and G10A, G14U, G16U, C20U, C52A

Promoter

h7SK

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pF3BGX-mTurquoise2

Plasmid

#138390

Purpose

RMCE vector for generating C-terminal mTurqouise2 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

mTurquoise2

Expression

Insect

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T-1-3xVSVG-GSK3(1-97)

Plasmid

#129582

Purpose

Encodes VSVG tagged GSK3(1-97) for purification by GST affinity

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Insert

GSK3a(1-97) (GSK3A Human)

Tags

GST and VSVG

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-29b-3xVSVG-GSK3(1-97)

Plasmid

#129583

Purpose

Encodes VSVG tagged GSK3(1-97) for purification by nickel affinity

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Insert

GSK3a(1-97) (GSK3A Human)

Tags

6xHistidine

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
kank1b_pJet1.2

Plasmid

#121979

Purpose

for generating kank1b riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank1b (si:dkey-226m8.9 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-lyzC-RFP-miR-30 backbone

Plasmid

#97147

Purpose

As a control of miRNA expression

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbS8k-RFP_K102E_E115K

Plasmid

#109405

Purpose

RepA Mutant

Depositor

Jay Keasling

Article

Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

Insert

repA

Tags

RFP

Expression

Bacterial

Mutation

K102E and E115K repA mutations to increase copy n…

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbS8k-RFP_N99K

Plasmid

#109409

Purpose

RepA Mutant

Depositor

Jay Keasling

Article

Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

Insert

repA

Tags

RFP

Expression

Bacterial

Mutation

N99K repA mutation to increase copy number

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 31,648 results for: REP