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Showing: 2851 - 2900 of 6278 results

  6. vDip-C: pAAV-Pcp2-mGreenLantern-KASH

    Plasmid
    #207613
    Purpose
    vDip-C AAV vector with mouse Pcp2 (also known as L7) promoter driving green fluorophore mGreenLantern with a KASH nuclear membrane tag to isolate cell nuclei of cerebellar Purkinje cells
    Depositor
    Longzhi Tan
    Article
    Tan et al Science. 2023 Sep 8;381(6662):1112-1119.
    Insert
    mGreenLantern
    Use
    AAV
    Tags
    KASH
    Expression
    Mutation
    Promoter
    mouse Pcp2 promoter (L7-6)
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    207613-plasmid-map-sequence-id-409730

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  8. AAV-EF1α1.1-FLPX-rc [Chronos-tdTomato]

    Plasmid
    #122103
    Purpose
    AAV-mediated expression of Chronos-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    Chronos-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a（1.1kb short version)
    Available since
    Sept. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    122103-plasmid-map-sequence-id-256678

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  9. pAAV-Syn-Flex-TREx2-tTA

    Plasmid
    #191210
    Purpose
    Cre dependent TRE-tTA expression under the control of Syn promoter
    Depositor
    Janelia Viral Tools
    Insert
    tTA
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    191210-plasmid-map-sequence-id-374532

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  12. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid
    #129531
    Purpose
    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR
    Depositor
    Erik Sontheimer
    Article
    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d
    Inserts
    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    Use
    AAV
    Tags
    FLAG/NLS
    Expression
    Mutation
    Promoter
    U6 and cytomegalovirus-enhancer chicken β-actin p…
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    129531-plasmid-map-sequence-id-255632

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  15. pAAV-EF1α1.1-FLEX-rc [ChrimsonR-GFP]

    Plasmid
    #108273
    Purpose
    AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    ChrimsonR-GFP
    Use
    AAV
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1α promoter (1.1kb short version)
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    108273-plasmid-map-sequence-id-213551

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  20. pAAV.CMV.SV40.THBS1-deltaCC-HA.SV40(polyA)

    Plasmid
    #155195
    Purpose
    Expresses murine Thrombospondin-1 (THBS1) protein with deletion of Coiled Coil domain and HA-tag at C-terminus
    Depositor
    Kevin Park
    Article
    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d
    Insert
    Thrombospondin-1 (Thbs1 Mouse)
    Use
    AAV
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    THBS1 with deletion of amino acid residues 276-315
    Promoter
    CMV
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    155195-plasmid-map-sequence-id-309128

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  21. pAAV-EF1α1.1-FLEX-rc [Chronos-GFP]

    Plasmid
    #84485
    Purpose
    AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    Chronos-GFP
    Use
    AAV
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α, 1.1 kb long (short version)
    Available since
    April 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    84485-plasmid-map-sequence-id-176951

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  22. pAAV-CAG-Flex-Rev-TdTomato

    Plasmid
    #122501
    Purpose
    Cre dependent TdTomato expression.
    Depositor
    Kimberly Ritola
    Insert
    TdTomato
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    122501-plasmid-map-sequence-id-237805

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  25. pAAV-hSyn-sbGLuc-CheRiff-EGFP

    Plasmid
    #114108
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Ikefuama et al Int J Mol Sci. 2022 Oct 27;23(21):12994.
    Insert
    Gaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    114108-plasmid-map-sequence-id-252563

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  33. pAAV-CAG-FLEX-splitTVA-EGFP

    Plasmid
    #59332
    Purpose
    Expresses splitTVA-P2A-EGFP
    Depositor
    Ian Wickersham
    Insert
    splitTVA950-P2A-EGFP
    Use
    AAV and Cre/Lox
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    59332-plasmid-map-sequence-id-92028

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  40. pAAV-CMV-dSa VP64 Actc1

    Plasmid
    #99691
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Actc1 promoter, vector allows for activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG) (Actc1 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    99691-plasmid-map-sequence-id-194530

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  46. pAAV-CMV-dSa VP64 Neurog2

    Plasmid
    #99695
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Sept. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    99695-plasmid-map-sequence-id-194570

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Showing: 2851 - 2900 of 6278 results