We narrowed to 164,825 results for: DES
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HA.PKD.S738A/S742A
Plasmid#10814DepositorInsertPKD (PRKD1 Human)
TagsHAExpressionMammalianMutationActivation loop inactive: S738A and S742A; L520PPromoterCMVAvailable SinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT
Plasmid#87259PurposeYFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133DepositorInsertsYFP-mAID-3HA
HXGPRT
UseToxoplasma expressionTags3HA and mAIDMutationCodon optimized for Toxoplasma gondii expressionPromoterTgDHFR-TS 463 bp and TgTUB1 378 bpAvailable SinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-K
Plasmid#81193PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below., Please view…PromoterROX3 and SNP52pAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA
Plasmid#37090PurposeExpresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitationDepositorInserthChr2(H134R)-mCherry
UseAAV and Cre/Lox; Cre-offTagsmCherryPromoterEF1aAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AU1-mTOR-L1460P
Plasmid#19995DepositorInsertmammalian target of rapamycin-L1460P (Mtor Rat)
TagsAU1ExpressionMammalianMutationChanged Leucine 1460 to ProlineAvailable SinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
SPACE (pRZ1813)
Plasmid#140242PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorInsertSPACE
ExpressionMammalianMutationV82G in TadA*, D10A in Cas9, R187W in pmCDA1PromoterCMVAvailable SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
shHIF2α(#2) (HIF2α-#2-pSUPER-retro-GFP/Neo)
Plasmid#22131DepositorInsertsynthethic oligonucleotide
UseRNAi and RetroviralExpressionMammalianMutationsynthethic oligonucleotide encoding shRNA targeti…Available SinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterNative promoter from pMUT2 relB/relE operon and P…Available SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
WT Smarca4-V5 IRES-puro
Plasmid#107058PurposeExpression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsIRES-puroR and V5ExpressionMammalianMutationwild-typePromoterCAGAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHEE401
Plasmid#71286PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistanceDepositorInsertssgRNA scaffold
zCas9
UseCRISPR; Plant binary vectorTags3x FLAG and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterEC1.2 promoter and U6-26p Arabidopsis U6 gene pro…Available SinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag_HsIRE1a_pBabePuro
Plasmid#54337PurposeN-terminally FLAG tagged human IRE1a in retroviral pBabe puro vectorDepositorInsertIRE1a (ERN1 Human)
UseRetroviralTagsFLAG and preprotrypsin signal sequenceExpressionMammalianMutationThe endogenous IRE1a signal sequence (aa1-18) was…Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ERBB2 A775_G776insYVMA
Plasmid#40982DepositorAvailable SinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
AMPK-β1-FLAG
Plasmid#40602DepositorAvailable SinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgA1/κ
Plasmid#61888PurposeExpresses HER2/neu receptor specific humanized IgA1/κ antibody isotype (Trastuzumab-IgA1/κ)DepositorInsertsHER2/neu receptor specific humanized Alpha 1 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
ExpressionMammalianPromotermEF1 Prom and rEF1 PromAvailable SinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
delta DE OCT4-GFP-2A-PURO transgenic reporter
Plasmid#52381PurposeBAC recombineering generated construct, used as a reporter for OCT4 expressionDepositorInsertsExpressionMammalianMutationdeleted -3194 - -1768 upstream from OCT4 ATGPromoterOCT4Available SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNTI194
Plasmid#84266PurposeExpresses Avi-tagged RPL1 and humanized birADepositorInsertsTagsAvi tag, Flag, Gly/Ser linker, and TEV siteExpressionMammalianMutationR33A, A34E (*see below)PromoterCMV and P2A peptideAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA Flag PGC1 T177A S538A
Plasmid#18093DepositorAvailable SinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Δ18 B.1.617.2v1
Plasmid#179905PurposeEncodes SARS-CoV-2 B.1.617.2v1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
ExpressionMammalianMutationT19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…PromoterCAGAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only
