165,775 results

1. pST_116_LVL2 cam

   Plasmid

   #179332

   Purpose

   NT-CRISPR plasmid for a single gRNA.

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
   

   Use

   CRISPR

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpCas9-TadCBEd-ivt-template

   Plasmid

   #193843

   Purpose

   Template for in vitro transcription of TadCBEd (with SpCas9)

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadCBEd with SpCas9
   

   Use

   In vitro transcription template

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AKARet-cyto

   Plasmid

   #96897

   Purpose

   FLIM sensor for PKA activation in cytosol

   Depositor

   Ryohei Yasuda

   Article

   Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

   Insert

   FLIM Sensor for PKA Activity in Cytosol
   

   Tags

   EGFP and sREAChet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28HIS-hAPE1

   Plasmid

   #70757

   Purpose

   bacterial expression of human APE1

   Depositor

   Primo Schaer

   Article

   Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

   Insert

   APEX1 (APEX1 Human)
   

   Tags

   6-HIS

   Expression

   Bacterial

   Promoter

   T7/lac operon

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.4_7C11-1 light chain

   Plasmid

   #198252

   Purpose

   recombinant expression of light chain of monoclonal anti-AMP antibody 7C11-1

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   light chain of monoclonal anti-AMP antibody 7C11-1 (mouse kappa)
   

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.4_7C11-1 heavy chain

   Plasmid

   #198251

   Purpose

   recombinant expression of heavy chain of monoclonal anti-AMP antibody 7C11-1

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   heavy chain of monoclonal anti-AMP antibody 7C11-1 (mouse IgG2a)
   

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-deadMalibu-mScarlet

   Plasmid

   #229555

   Purpose

   Fluorescent reporter for malonyl-CoA that does not bind malonyl-CoA (control)

   Depositor

   Danielle Schmitt

   Article

   Ranzau et al bioRxiv [Preprint]. 2024 Sep 28:2024.09.

   Insert

   Malibu-mScarlet
   

   Tags

   mScarlet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57-Cx46-IRES-GCaMP6s

   Plasmid

   #188238

   Purpose

   3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 46 and GCaMP6s

   Depositor

   Fabio Mammano

   Article

   Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

   Insert

   GJA3 (GJA3 Human)
   

   Use

   Lentiviral

   Tags

   GCaMp6s

   Expression

   Mammalian

   Promoter

   Tight TRE promoter

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-SIN-BX SpyCatcher003-hCD52

   Plasmid

   #210565

   Purpose

   Expresses Spycatcher003 on the cell surface using human CD52 hinge

   Depositor

   Omer Dushek

   Article

   Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

   Insert

   Spycatcher003 fused to human CD52 hinge
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCVpuro-mTagBFP2

    Plasmid

    #96935

    Purpose

    Retroviral plasmid containing mTagBFP2 for labeling of cells

    Depositor

    Lee Grimes

    Article

    Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

    Insert

    Monomeric Tag Blue Fluorescent Protein
    

    Expression

    Mammalian

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHCMV_RVFV_GP

    Plasmid

    #207304

    Purpose

    Mammalian expression of Rift valley fever virus GP protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    RVFV_GP (RVFV_sM_gp1 Rift valley fever virus)
    

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mKeima-Red-N1

    Plasmid

    #54597

    Purpose

    Localization: N1 Cloning Vector, Excitation: 440, Emission: 620

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    mKeima-Red

    Expression

    Mammalian

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-hMIGA2-DsRed

    Plasmid

    #192866

    Purpose

    Express codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Tags

    DsRed

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PCDNA3-S1P1-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

    Plasmid

    #113612

    Purpose

    PPI reporter

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

    Insert

    S1P1
    

    Expression

    Mammalian

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PGK-AAVS1ZFNL

    Plasmid

    #60916

    Purpose

    encodes zinc finger nuclease specific to AAVS1 locus (left)

    Depositor

    Paul Gadue

    Article

    Sim et al Methods Mol Biol. 2015 Jan 29.

    Insert

    AAVS1-ZFNL
    

    Promoter

    PGK

    Available Since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBABE hBRM

    Plasmid

    #1961

    Depositor

    Robert Kingston

    Article

    Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

    Insert

    BRM (SMARCA2 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. Luc.Cre empty vector

    Plasmid

    #20905

    Depositor

    Tyler Jacks

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-NSP5SA11

    Plasmid

    #89172

    Purpose

    Express Rotavirus SA11 NSP5 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP5
    

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-SENP5

    Plasmid

    #34555

    Depositor

    Mary Dasso

    Article

    Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

    Insert

    SENP5 (SENP5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pNR291

    Plasmid

    #79555

    Purpose

    Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Inserts

    GFPmut3b
    mTagBFP
    mRFP
    

    Expression

    Bacterial

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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