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pAAV-OTp-mCherry

Plasmid

#184754

Purpose

To drive mCherry under the control of mouse oxytocin promoter

Depositor

Kazunari Miyamichi

Article

Inada et al Neuron. 2022 Apr 12. pii: S0896-6273(22)

Insert

mCherry

Use

AAV

Promoter

mouse Oxytocin promoter

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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iRFP-H2A

Plasmid

#158691

Purpose

This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

iRFP670

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSF279

Plasmid

#167674

Purpose

Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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LV-Cre pLKO.1

Plasmid

#25997

Purpose

Lentiviral expression of nls-Cre from the CMV promoter

Depositor

Elaine Fuchs

Article

Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

Insert

Cre recombinase

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pNIC28-BIRC3

Plasmid

#196366

Purpose

protein expression of the His-tagged full length protein

Depositor

Stefan Knapp

Insert

BIRC3/cIAP2

Tags

His6-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pT040

Plasmid

#67640

Purpose

URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

Depositor

John Wyrick

Article

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Insert

single guide RNA expression cassette

Use

CRISPR

Expression

Yeast

Promoter

pSNR52

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-KIR2DL5A-COMP5AP-AviTag-9xHis

Plasmid

#157207

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL5A (KIR2DL5A Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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SKIV2L

Plasmid

#156014

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SKIV2L (SKIC2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

D3E

Promoter

EF-1a

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt12

Plasmid

#184513

Purpose

Mammalian expression of Synaptotagmin 12 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 12 (Syt12 Rat)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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VAMP4-SEP

Plasmid

#174406

Purpose

Reporter for VAMP4 exocytosis in mammalian cells

Depositor

David Perrais

Article

Bakr et al Cell Rep. 2021 Sep 7;36(10):109678. doi:

Insert

vesicle associated membrane protein 4 (VAMP4 Human)

Tags

superecliptic phulorin (mutated GFP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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TMEM16F-M522K-peGFP-N1

Plasmid

#228579

Purpose

Expresses mouse TMEM16F-M522K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

M522K plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4H5

Plasmid

#71716

Purpose

Membrane scaffold protein (MSP), helix 4+ helix 5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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GW1-Mito-pHRed

Plasmid

#31474

Depositor

Gary Yellen

Article

Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

Insert

Cox8-tagged pHRed

Tags

4xCox8

Expression

Mammalian

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-Dendra2

Plasmid

#29574

Depositor

Anna Huttenlocher

Article

Yoo et al J Leukoc Biol. 2011 May;89(5):661-7. doi

Insert

mpx-Dendra 2

Available Since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only
Anti-Shank3 [N69/46R]

Plasmid

#140078

Purpose

Mammalian Expression Plasmid of anti-Shank3 (Rat). Derived from hybridoma N69/46.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Shank3 (Rat) recombinant mouse monoclonal antibody (Shank3 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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PB53x EF1-Dendra2-RPB1Amr

Plasmid

#81228

Purpose

expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system

Depositor

Timothée Lionnet

Article

Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1

Insert

Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)

Tags

Dendra2

Expression

Mammalian

Mutation

N792D alpha amanitin resistance mutation (Bartolo…

Promoter

EF1a

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-ecfp

Plasmid

#65030

Purpose

GmR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

ecfp

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pUC57-PB-PGK-tetR-KRAB-2A-Puro

Plasmid

#129536

Purpose

PiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.

Depositor

Hideyuki Okano

Article

Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

Insert

PB-PGK-tetR-KRAB-2A-Puro

Use

Unspecified

Promoter

hPGK promoter

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Stat3-C Flag pRc/CMV

Plasmid

#8722

Depositor

Jim Darnell

Article

Bromberg et al Cell 1999 Aug 6;98(3):295-303.

Insert

Stat3-C (Stat3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

A662C and N664C. Residues changed to Cys renders…

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FGF1-4X

Plasmid

#120282

Purpose

Produce human recombinant FGF1-4X in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Basic fibroblast growth factor 1 mutant

Tags

6x His tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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