We narrowed to 13,697 results for: TIM

1. csr-1_son-1_mCherry

   Plasmid

   #191742

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Use

   Tags

   NCU04502 promoter and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHT-trxA.ads

   Plasmid

   #47383

   Purpose

   Expresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Insert

   ads
   

   Use

   Tags

   6xHis and trxA

   Expression

   Bacterial

   Mutation

   Promoter

   Pgrac

   Available since

   Oct. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. tubulin-CeNL_pcDNA3

   Plasmid

   #89515

   Purpose

   Cyan color variant of bright luminescent protein, fused to b-tubulin

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   CeNL
   

   Use

   Tags

   b-tubulin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BCJJ340

   Plasmid

   #117502

   Purpose

   UBI10:Cas9:E9 Golden Gate Level 1 Position 2

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUBI10

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiCRISPRv2-7SK-Blast

   Plasmid

   #183620

   Purpose

   lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.

   Depositor

   Bill Sugden

   Article

   Hutcheson et al ACS Synth Biol. 2022 Jun 29. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Modified 7SK

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSUPER-retro-puro-shNup133-1

   Plasmid

   #87334

   Purpose

   To express shRNA against human Nup133

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   shRNA against human Nup133
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCIS.MTRIA-PRLH

   Plasmid

   #184618

   Purpose

   For expression of a fluorescent sensor for Prolactin-releasing peptide factor in mammalian cells

   Depositor

   Daisuke Ino, Hiroshi Hibino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-PRLH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCIS.MTRIA-NOC

   Plasmid

   #184611

   Purpose

   For expression of a fluorescent sensor for nociceptin in mammalian cells

   Depositor

   Daisuke Ino, Hiroshi Hibino

   Article

   Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

   Insert

   MTRIA-NOC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YN2_1_LT84_RPL13A

   Plasmid

   #177712

   Purpose

   Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. YeNL-Actin_pcDNA3

    Plasmid

    #89521

    Purpose

    Yellow color variant of bright luminescent protein, fused to actin

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    YeNL
    

    Use

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNB777

    Plasmid

    #60153

    Purpose

    Red click beetle luciferase (Promega) driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    CBR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    LEU1pr

    Available since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR-L4-pTF-L1R

    Plasmid

    #45954

    Depositor

    Patrick Salmon

    Article

    Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

    Insert

    pTF promoter
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    TET optimized

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pME-QF2w-SV40pA

    Plasmid

    #155117

    Purpose

    Middle entry vector containing QF2w

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QF2w
    

    Use

    Gateway middle entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. tubulin-YeNL_pcDNA3

    Plasmid

    #89519

    Purpose

    Yellow color variant of bright luminescent protein, fused to b-tubulin

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    YeNL
    

    Use

    Tags

    b-tubulin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KA2897_pPB-TREtight-UBCmin-cHA-IRESH2BmKO2

    Plasmid

    #124176

    Purpose

    piggyBac-based Dox-inducible expression (moderate)

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYFP-lacY-1

    Plasmid

    #140138

    Purpose

    Evaluating the impact of feedback on information transmission of a genetic sensor.

    Depositor

    Drew Tack

    Article

    Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

    Insert

    lacY (lacY )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMX Vav1 Y3F/PH* (W495L) uGFP

    Plasmid

    #14564

    Depositor

    Joan Brugge

    Article

    Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

    Insert

    Vav1 (VAV1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PH* (W495L)

    Promoter

    Available since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pHFX-cas3UD

    Plasmid

    #174385

    Purpose

    Plasmids for gene knockout were constructed based on suicide plasmid pHFX

    Depositor

    Jing Han

    Article

    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

    Insert

    Cas3UD
    

    Use

    Archaeal gene knockout vector for haloferax medit…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMX Vav1 Y3F/CR* (C529S) uGFP

    Plasmid

    #14560

    Depositor

    Joan Brugge

    Article

    Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

    Insert

    Vav1 (VAV1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Y3F/CR* (C529S)

    Promoter

    Available since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pSox2-bd::FP

    Plasmid

    #34703

    Depositor

    Hollis Cline

    Article

    Bestman et al J Comp Neurol. 2012 Feb 1;520(2):401-33.

    Insert

    eGFP
    

    Use

    Xenopus expression

    Tags

    165-bp minimal FGF4 promoter, 6x Sox2/Oct3-4 tran…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. OeNL-H2B_pcDNA3

    Plasmid

    #89527

    Purpose

    Orange color variant of bright luminescent protein, fused to histone 2B

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    OeNL
    

    Use

    Tags

    histone 2B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUC19-U6-crRNA1

    Plasmid

    #123639

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    crRNA scaffold 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. RV-Syn-Dio-GFP

    Plasmid

    #87673

    Purpose

    retroviral vector encoding cre dependent expression of GFP

    Depositor

    Oscar Marin

    Article

    Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

    Insert

    eGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAW8ENdeI-cHAIAA7-spo5DSR

    Plasmid

    #49873

    Purpose

    for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    IAA17 degron tag

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. KA1130_pPB-TREtight-cHA-IRESH2BmCherry

    Plasmid

    #124173

    Purpose

    piggyBac-based Dox-inducible expression

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLentiCRISPRv1.linker_KO

    Plasmid

    #164688

    Purpose

    For CRISPR knockout of miR-144~451 linker region by lentiviral delivery of Cas9 and linker gRNA

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    miR-144~451 linker CRISPR KO gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSUPER-retro-puro-shNup133-2

    Plasmid

    #87333

    Purpose

    To express shRNA against human Nup133

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    shRNA against human Nup133
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. OeNL-Actin_pcDNA3

    Plasmid

    #89524

    Purpose

    Orange color variant of bright luminescent protein, fused to actin

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    OeNL
    

    Use

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMOS030: ratiometric pHluorin (endoplasmic reticulum)

    Plasmid

    #163054

    Purpose

    ratiometric pHluorin (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    ratiometric pHluorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJ414 - DANCER-3

    Plasmid

    #136387

    Purpose

    Expresses DANCER-3 dynamic protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    DANCER-3
    

    Use

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-A34F-V39L-V54I

    Promoter

    T7 promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRSET FLII32Pglu-2.2m

    Plasmid

    #13566

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A P32S-ECFP-D33R
    

    Use

    Tags

    6xHis and Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUCBB-lacZa

    Plasmid

    #32563

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    LacZ-alpha
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. KA2706_eSpCas9-PGKPacdtkCh

    Plasmid

    #124204

    Purpose

    Expression of eSpCas9(1.1) and sgRNA

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYFP-x-1

    Plasmid

    #140142

    Purpose

    Evaluating information transmission of a genetic sensor.

    Depositor

    Drew Tack

    Article

    Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

    Insert

    lacY (lacY )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D37, I38, and N39 are changed to nonsense (stop) …

    Promoter

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pYPQ158

    Plasmid

    #69307

    Purpose

    Gateway entry vector with hSpCas9 (Human codon optimized)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    hSpCas9 (Human codon optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mutation

    NLS fusion

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSox2-bd_mFGF_eGFP

    Plasmid

    #34704

    Depositor

    Hollis Cline

    Article

    Bestman et al J Comp Neurol. 2012 Feb 1;520(2):401-33.

    Insert

    eGFP
    

    Use

    Xenopus expression

    Tags

    165-bp minimal FGF4 promoter and 6x Sox2/Oct3-4 t…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mEmerald-PCNA-19-NLS-4

    Plasmid

    #54222

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

    Insert

    PCNA-19 NLS (PCNA Human)
    

    Use

    Tags

    SV40NLS and mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

38. B3rec.zf1

    Plasmid

    #108354

    Purpose

    Zebrafish codon optimized B3 recombinase that acts on blown-out sites

    Depositor

    Harold Burgess

    Article

    Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.

    Insert

    B3 recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Zebrafish codon optimized

    Promoter

    lac

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pNB763

    Plasmid

    #60158

    Purpose

    Firefly luciferase (Promega) driven by LYS9 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    LYS9pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMK33/intein-Cas9_S219-3XFLAG

    Plasmid

    #133782

    Purpose

    Expresses human codon-optimized intein-Cas9

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

    Insert

    Human codon-optimized intein-Cas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge