We narrowed to 18,831 results for: Tec

1. pBBR6k-J23101-GFPuv

   Plasmid

   #106390

   Purpose

   pBBR6k-GFPuv with Anderson promoter J23101 expressing lacI

   Depositor

   Brian Pfleger

   Article

   Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

   Insert

   GFPuv
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LlacO-1

   Available Since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. V621 pGEX-4T-LP - Acceptor

   Plasmid

   #11715

   Depositor

   Tony Pawson

   Article

   Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

   Type

   Empty backbone

   Use

   Cre/Lox; Acceptor vector

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 24, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. BCJJ125

   Plasmid

   #117515

   Purpose

   Cas9_3 (with intron) Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:AATG:Cas9_3(intron):GCTT:BsaI
   

   Use

   CRISPR

   Tags

   2xFLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGC4593

   Plasmid

   #110206

   Purpose

   Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

   Depositor

   Adam Arkin, Guillaume Cambray

   Article

   Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

   Inserts

   TEV protease
   aaRS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pbad and Ptet

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1C-YFP-shSCR

   Plasmid

   #139647

   Purpose

   Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

   Depositor

   David Cobrinik

   Article

   Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

   Insert

   shSCR
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor comments

   Promoter

   CMV

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. EMM65

   Plasmid

   #49042

   Purpose

   Backbone contains SHD/C 0.5 Domain TALE N-terminal: TAL C-terminus: LSD1: Isoform 2

   Depositor

   Eric Mendenhall, Bradley Bernstein

   Article

   Mendenhall et al Nat Biotechnol. 2013 Sep 8. doi: 10.1038

   Type

   Empty backbone

   Use

   Tale lsd1 expression vector

   Tags

   LSD1

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 alpha

   Available Since

   Oct. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCellFree_G03 PC

   Plasmid

   #67132

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PC (PC Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pNH051

   Plasmid

   #42157

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   GFP-RT-GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUSE-RapR-Src-myc

   Plasmid

   #25933

   Depositor

   Klaus Hahn

   Article

   Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

   Insert

   RapR-Src (Src Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. PlayBack-Ef1aKpn1

    Plasmid

    #203307

    Purpose

    For expression of eukaryotic genes of interest with Ef1a promoter with Kpn1 as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSIBR4-Cre

    Plasmid

    #219435

    Purpose

    Theophylline_induced self_splicing intron_based riboswitch for the moderate expression of Cre recombinase

    Depositor

    Sheng Yang

    Article

    Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

    Insert

    intron 4-Cre
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCOLA-T7-WspR:D70E

    Plasmid

    #79164

    Purpose

    Expresses constitutively active WspR D70E under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    WspR (wspR P. areuginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D70E

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRBBm59

    Plasmid

    #48115

    Purpose

    Shuttle vector E. coli/B.meg.

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

    Insert

    promoter sacB - gfp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NLS-LgBiT-CaM

    Plasmid

    #183401

    Purpose

    Expresses nuclear-localized LgBiT-CaM

    Depositor

    Alice Ting

    Article

    Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

    Insert

    LgBiT-CaM
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCfB2196

    Plasmid

    #67549

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked bleMX marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MOBE3

    Plasmid

    #219943

    Purpose

    Mammalian expression of MOBE3 protein (apt-CBE-SL3_PT2A_apt-ABE8e)

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    apt-CBE-SL3_PT2A_apt-ABE8e
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GGE146

    Plasmid

    #120742

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_4UASxpr-TEF)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    P1_4UASxpr-TEF
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 438-GST

    Plasmid

    #97092

    Purpose

    GST-tev-yORF

    Depositor

    Chris Jeans

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    GST tag and TEV cleavage site

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin promoter

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBad33_ISEc41_reRNA

    Plasmid

    #205341

    Purpose

    Bacterial expression of ISEc41 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISEc41 TnpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBad33_ISEc46_reRNA

    Plasmid

    #205343

    Purpose

    Bacterial expression of ISEc46 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISEc46 TnpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBad33_ISNsp2_reRNA

    Plasmid

    #205350

    Purpose

    Bacterial expression of ISNsp2 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISNsp2 TnpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHG4

    Plasmid

    #188252

    Purpose

    Integrating plasmid contains the TG1 int/attP locus and the apramycin-resistance gene (aac(3)IV) for selection

    Depositor

    Maggie Smith, Hong Gao

    Article

    Gao et al BMC Biotechnol. 2019 Jun 4;19(1):32. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    act1p/actII-orf4

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBBR6k-GFPuv

    Plasmid

    #106384

    Purpose

    IPTG-inducible LlacO-1 promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LlacO-1

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBYK01

    Plasmid

    #138656

    Purpose

    phiOZJ integrating Streptomyces expression vector lacking RDF

    Depositor

    Joshua Blodgett

    Article

    Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PermE* variant sequence from pDA1652

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMAL-c4E

    Plasmid

    #75293

    Purpose

    Expresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

    Depositor

    Paul Riggs

    Article

    Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

    Type

    Empty backbone

    Use

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    July 19, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. PE-GsI-IIC-RT

    Plasmid

    #181808

    Purpose

    PE-GsI-IIC RT

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

    Insert

    PE-GsI-IIC-RT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJMD5

    Plasmid

    #138652

    Purpose

    phiBT1 integrating Streptomyces expression vector

    Depositor

    Joshua Blodgett

    Article

    Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PermE* variant sequence from pDA1652

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMET17p-CRU313

    Plasmid

    #131163

    Purpose

    For ScMET17 promoter regulated expression of Cub-R-URA3 fused bait proteins, centromeric ARS plasmid, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutant

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

    Type

    Empty backbone

    Use

    Tags

    CRU

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GGE104

    Plasmid

    #120744

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position1 (P1_EYK300-3AB)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    P1_EYK300-3AB
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCfB2193

    Plasmid

    #67542

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-pnGFP

    Plasmid

    #64913

    Purpose

    Encodes a Fluorescent Reporter for Peroxynitrite in Mammalian Cells

    Depositor

    Huiwang Ai

    Article

    Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

    Insert

    pnGFP
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Chromophore tyrosine of the protein mutated to TA…

    Promoter

    CMV

    Available Since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pETDuet-T7-Ct-WspR

    Plasmid

    #79166

    Purpose

    Dual expression of Ct biosensor and WspR WT under individual T7 promoters

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Inserts

    WspR (wspR P. areuginosa)
    T7-Spinach2-Ct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHCKAn-phoAp-GRFT

    Plasmid

    #158747

    Purpose

    low phosphate phoAp induction of Griffithsin

    Depositor

    Michael Lynch

    Article

    Decker et al Front Bioeng Biotechnol. 2020 Aug 21;8:1

    Insert

    phoAp Griffithsin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    phoA

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTYF-1xGfaABC1D-EGFP

    Plasmid

    #19974

    Depositor

    Sergey Kasparov

    Article

    Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

    Insert

    GfaABC1D-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRSETb-tVYN-TmΔ

    Plasmid

    #149694

    Purpose

    High-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purification

    Depositor

    Ming Hammond

    Article

    Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

    Insert

    VenusΔC12-TmYcgRΔ-NLucΔ4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLenti-EF1a-TALE1

    Plasmid

    #27969

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    TALE1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBC2103-reci

    Plasmid

    #117172

    Purpose

    U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    reci_arCas9 without NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pU6-Fn-crRNA

    Plasmid

    #78958

    Purpose

    Cloning vector for expression of FnCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEL856

    Plasmid

    #214256

    Purpose

    Expression TadDE-NG in rice cells

    Depositor

    Yong Zhang

    Article

    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG-i53

    Plasmid

    #119002

    Purpose

    Mammalian expression of i53 (53BP1) inhibitor and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    i53
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge