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Showing: 2901 - 2950 of 2952 results

  3. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-TAILLESS

    Plasmid
    #221117
    Purpose
    FAP tagged STE3-TAILLESS mutant (delta288-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER
    Depositor
    Allyson O’Donnell
    Article
    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi
    Insert
    MFA-IgKappa-FAPoptim-STE3-Tailless
    Use
    Tags
    Expression
    Yeast
    Mutation
    STE3 mutant that lacks the entire C-terminal tail…
    Promoter
    STE3
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    221117-plasmid-map-sequence-id-466649

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  4. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-deltaPEST

    Plasmid
    #221118
    Purpose
    FAP tagged STE3-PEST mutant (delta413-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER
    Depositor
    Allyson O’Donnell
    Article
    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi
    Insert
    MFA-IgKappa-FAPoptim-STE3-deltaPEST
    Use
    Tags
    Expression
    Yeast
    Mutation
    STE3 mutant that lacks the PEST domain in the C-t…
    Promoter
    STE3
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    221118-plasmid-map-sequence-id-442464

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  7. pPBbsr2-EREG-ScNeo

    Plasmid
    #209909
    Purpose
    To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    EREG-ScNeo (EREG Human)
    Use
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209909-plasmid-map-sequence-id-416692

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  8. pPBbleo-TGFa-EREG-ScNeo

    Plasmid
    #209915
    Purpose
    To express the chimeric protein of TGFα and EREG, which a recombinant TGFα protein fused extracellularly to the mScarlet and a recombinant Epiregulin protein fused intracellularly to the mNeonGreen
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    TGFa-EREG-ScNeo (EREG, TGFA Human)
    Use
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209915-plasmid-map-sequence-id-416705

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  9. pPBbleo-EREG-ScNeo

    Plasmid
    #209905
    Purpose
    To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    EREG-ScNeo (EREG Human)
    Use
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209905-plasmid-map-sequence-id-416688

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  10. pAW-BiP-Nb127D01-mCherry-KDEL

    Plasmid
    #171575
    Purpose
    Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane
    Depositor
    Norbert Perrimon, Hidde Ploegh
    Article
    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:
    Insert
    BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)
    Use
    Tags
    BiP signal peptide and mCherry-KDEL
    Expression
    Insect
    Mutation
    Promoter
    fly actin5C promoter
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    171575-plasmid-map-sequence-id-339963

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  11. pAW-BiP-NbVHH05-mCherry-KDEL

    Plasmid
    #171574
    Purpose
    Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane
    Depositor
    Norbert Perrimon, Hidde Ploegh
    Article
    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:
    Insert
    BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)
    Use
    Tags
    BiP signal peptide and mCherry-KDEL
    Expression
    Insect
    Mutation
    Promoter
    fly actin5C promoter
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171574-plasmid-map-sequence-id-336399

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  12. TFORF3305

    Plasmid
    #144781
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ESR1 (ESR1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144781-plasmid-map-sequence-id-404373

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  13. TFORF1954

    Plasmid
    #142296
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ESR2 (ESR2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142296-plasmid-map-sequence-id-395774

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  14. TFORF3132

    Plasmid
    #144608
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ESR2 (ESR2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144608-plasmid-map-sequence-id-346764

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  15. TFORF1955

    Plasmid
    #142297
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ESR2 (ESR2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    142297-plasmid-map-sequence-id-454963

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  16. TFORF1956

    Plasmid
    #142898
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ESR2 (ESR2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142898-plasmid-map-sequence-id-396065

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  17. TFORF1957

    Plasmid
    #142298
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ESR2 (ESR2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142298-plasmid-map-sequence-id-395775

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  18. pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL

    Plasmid
    #73209
    Purpose
    Expresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    kappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Human, Synthetic)
    Use
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73209-plasmid-map-sequence-id-139734

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  25. str-KDEL_Stx5L(M55V)-SBP-mCitrine

    Plasmid
    #209846
    Purpose
    Synchronize trafficking of Stx5L (M55V) from the ER, for use with FLIM (RUSH system)
    Depositor
    Geert van den Bogaart
    Article
    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:
    Insert
    STX5 (STX5 Human)
    Use
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Changed Methionine 55 to Valine, causes loss of S…
    Promoter
    CMV
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    209846-plasmid-map-sequence-id-413647

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  26. str-KDEL_Stx5S-SBP-mCitrine

    Plasmid
    #154845
    Purpose
    Synchronize trafficking of Stx5S from the ER, for use with FLIM (RUSH system)
    Depositor
    Geert van den Bogaart
    Article
    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:
    Insert
    Stx5 (Short isoform) (STX5 Human)
    Use
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Lacks amino acids 1-54, enocdes short isoform of …
    Promoter
    CMV
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    154845-plasmid-map-sequence-id-325019

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  31. str-KDEL_Stx5LdeltaER-SBP-mCitrine

    Plasmid
    #154846
    Purpose
    Synchronize trafficking of Stx5LdeltaER (M55V) from the ER, for use with FLIM (RUSH system)
    Depositor
    Geert van den Bogaart
    Article
    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:
    Insert
    Stx5 (Long isoform) (STX5 Human)
    Use
    Tags
    Streptavidin Binding Protein (SBP) and mCitrine
    Expression
    Mammalian
    Mutation
    Changed Methionine 55 to Valine, causes loss of S…
    Promoter
    CMV
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    154846-plasmid-map-sequence-id-348286

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  43. pAAV-Syn-SomaGCaMP6f1

    Plasmid
    #158755
    Purpose
    Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP6f1
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158755-plasmid-map-sequence-id-310657

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Showing: 2901 - 2950 of 2952 results