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Addgene

We narrowed to 3,025 results for: ER

Showing: 2901 - 2950 of 3025 results
  1. pLHCX-ATF4 mScarlet NLS

    Plasmid
    #115970
    Purpose
    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)
    Depositor
    Insert
    activating transcription factor 4 (ATF4 Human, Synthetic)
    Use
    Retroviral
    Tags
    HA tag, c-myc NLS, and mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST

    Plasmid
    #109048
    Purpose
    Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in a viral vector
    Depositor
    Has Service
    AAV9
    Insert
    eGtACR1-ST
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    July 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. eMscL G22S

    Plasmid
    #107455
    Purpose
    eMscL G22S is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif
    Depositor
    Insert
    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia coli)
    Use
    Adeno-associated viral
    Tags
    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…
    Expression
    Mammalian
    Mutation
    Bearing a glycine to serine substitution at posit…
    Promoter
    human synapsin 1
    Available since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. qTAG-C-Solo-mStayGold-CANX

    Plasmid
    #227280
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)
    Depositor
    Insert
    CANX Homology Arms flanking a mStayGold Tag (CANX Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. eMscL WT

    Plasmid
    #107454
    Purpose
    eMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif
    Depositor
    Insert
    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
    Use
    Adeno-associated viral
    Tags
    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin 1
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN

    Plasmid
    #99049
    Purpose
    Transcription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domain
    Depositor
    Inserts
    ACT1 promoter (ACT1 Budding Yeast)
    mCherry
    Zif268 DNA binding Domain
    Estrogen response domain (ESR1 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. pOUPc-UL148-mCherry

    Plasmid
    #179279
    Purpose
    All in one "tet-on" lentivirus vector that expresses human cytomegalovirus UL148 fused to an mCherry tag at its cytoplasmic tail. UL148 reorganizes the ER and activates the UPR.
    Depositor
    Insert
    UL148 fused to mCherry
    Use
    Lentiviral; Tet-on, all-in-one lentiviral vector
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Tet responsive element 3G
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

    Plasmid
    #140163
    Purpose
    Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker
    Depositor
    Insert
    GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Human, Synthetic)
    Use
    Tags
    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
  9. pBT3000

    Plasmid
    #122536
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (tram-1) under the intestinal specific promoter nhx-2
    Depositor
    Insert
    Pnhx-2_CemOrange2::tram-1 (tram-1 Nematode, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. pBT3003

    Plasmid
    #122537
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (pisy-1) under the intestinal specific promoter nhx-2
    Depositor
    Insert
    Pnhx-2_CemOrange2::pisy-1 (pisy-1 Nematode, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  11. dCas9-NLS-gs10-cERb447

    Plasmid
    #188257
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split ERbeta (aa 447-501), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Insert
    cERb447 (ESR1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. pOUPc-UL148-HA

    Plasmid
    #179343
    Purpose
    All-in-one "tet-on" 3G lentiviral vector plasmid encoding human cytomegalovirus UL148 ER resident glycoprotein with a C-terminal HA tag fused to its cytoplasmic tail
    Depositor
    Insert
    human cytomegalovirus UL148 fused to HA epitope
    Use
    Lentiviral and Synthetic Biology; Hiv-1 based rep…
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    insert matches UL148 from strain TB40/E
    Promoter
    Tet responsive element 3G
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  13. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3

    Plasmid
    #221112
    Purpose
    FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER
    Insert
    MFA-IgKappa-FAPoptim-STE3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    STE3
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pRS415-TEF1pr-MFA-Igkappa-FAPoptim-STE3

    Plasmid
    #221114
    Purpose
    FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Tef1 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER
    Insert
    MFA-IgKappa-FAPoptim-STE3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  15. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-K424R

    Plasmid
    #221119
    Purpose
    FAP tagged STE3-K424R mutant (N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER
    Insert
    MFA-IgKappa-FAPoptim-STE3-K424R
    Use
    Tags
    Expression
    Yeast
    Mutation
    STE3 mutantK424R
    Promoter
    STE3
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  16. CA245 CMVTO_ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry

    Plasmid
    #231206
    Purpose
    Single transcript cageIL-10 (mRens) + cleaveIL-6 (mRens) + ER-renin (H2M)
    Depositor
    Insert
    ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L147I, R148H, I203V, L290V
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 2901 - 2950 of 3025 results