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Showing: 2901 - 2950 of 8580 results

  3. 3` CC: Hygro – GFP-N – loxP – mScarlet

    Plasmid
    #219564
    Purpose
    3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosome
    Depositor
    Andrea Ventura
    Article
    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586
    Insert
    3` CC: Hygro – GFP-N – loxP – mScarlet
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    EF1-a
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219564-plasmid-map-sequence-id-438335

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  4. pEF2-FL-hTyro_gR1

    Plasmid
    #225995
    Purpose
    Expresses a chimeric contruct having N-terminal human Tyro3 and C-terminal human Interferon Gamma Receptor
    Depositor
    Sergei Kotenko, Raymond Birge
    Article
    Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6
    Insert
    human Tyro3 N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (TYRO3, IFNGR1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    225995-plasmid-map-sequence-id-454328

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  5. pEF2-FL-hMer_gR1

    Plasmid
    #225993
    Purpose
    Expresses a chimeric contruct having N-terminal human MerTK and C-terminal human Interferon Gamma Receptor
    Depositor
    Sergei Kotenko, Raymond Birge
    Article
    Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6
    Insert
    human MerTK N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (MERTK, IFNGR1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-a
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    225993-plasmid-map-sequence-id-454327

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  9. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid
    #193139
    Purpose
    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…
    Promoter
    EF1a
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193139-plasmid-map-sequence-id-380234

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  12. makeTCR_Lentiviral_Backbone

    Plasmid
    #233943
    Purpose
    backbone for makeTCR assembly
    Depositor
    Michael Platten
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1a (long)
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    233943-plasmid-map-sequence-id-469998

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  13. pEBB-SBP-HA

    Plasmid
    #36100
    Depositor
    Ezra Burstein
    Type
    Empty backbone
    Use
    Tags
    HA and SBP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    36100-plasmid-map-sequence-id-44719

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  14. pLX_TRC311

    Plasmid
    #113668
    Purpose
    Gateway destination vector for lentiviral expression
    Depositor
    John Doench
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    113668-plasmid-map-sequence-id-220922

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  15. BII-ChPtW-tdT

    Plasmid
    #133401
    Purpose
    Piggybac vector for constitutive expression of tdTomato
    Depositor
    Blair Madison
    Insert
    tdTomato
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133401-plasmid-map-sequence-id-268578

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  16. BII-ChPtW-iRFP702

    Plasmid
    #133398
    Purpose
    Piggybac vector for constitutive expression of iRFP702
    Depositor
    Blair Madison
    Insert
    IRFP-702
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133398-plasmid-map-sequence-id-267650

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  17. BII-ChPtW-iRFP670

    Plasmid
    #133397
    Purpose
    Piggybac vector for constitutive expression of iRFP670-NLS
    Depositor
    Blair Madison
    Insert
    iRFP-670-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    133397-plasmid-map-sequence-id-269430

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  18. BII-ChPtW-smURFP

    Plasmid
    #133399
    Purpose
    Piggybac vector for constitutive expression of smURFP-NLS
    Depositor
    Blair Madison
    Insert
    smURFP-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133399-plasmid-map-sequence-id-268584

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  19. lentiCRISPRv2_ZsG gRNA_4

    Plasmid
    #192688
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #4
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a, U6
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    192688-plasmid-map-sequence-id-379587

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  20. lentiCRISPRv2_ZsG gRNA_1

    Plasmid
    #192685
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a, U6
    Available since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192685-plasmid-map-sequence-id-379591

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  21. lentiCRISPRv2_ZsG gRNA_2

    Plasmid
    #192686
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a, U6
    Available since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192686-plasmid-map-sequence-id-379590

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  22. lentiCRISPRv2_ZsG gRNA_3

    Plasmid
    #192687
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #3
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a, U6
    Available since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192687-plasmid-map-sequence-id-379588

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  23. pDY0973-RADARS iCaspase

    Plasmid
    #182539
    Purpose
    Express RADARS iCaspase that conditionally turn on iCaspase in the presence of target RNA
    Depositor
    Jonathan Gootenberg, Omar Abudayyeh
    Insert
    iCaspase9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182539-plasmid-map-sequence-id-356646

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  24. pJY4092

    Plasmid
    #113334
    Purpose
    Vector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoter
    Depositor
    Suzanne Sandmeyer
    Insert
    codon-optimized Hermes transposase
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113334-plasmid-map-sequence-id-218285

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  25. piggyBAC-U6-GFP-WPRE

    Plasmid
    #200030
    Purpose
    piggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAs
    Depositor
    Jay Shendure
    Type
    Empty backbone
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6, EF1a
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    200030-plasmid-map-sequence-id-393204

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  26. BII-Sh-BnTW

    Plasmid
    #133365
    Purpose
    Piggybac vector for shRNA-mediated knockdown, with co-expression with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    Flag-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133365-plasmid-map-sequence-id-268571

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  27. pCMX-Gal4-TEAD1-D278K

    Plasmid
    #38106
    Depositor
    Kunliang Guan
    Insert
    TEAD1 (TEAD1 Human)
    Use
    Tags
    Gal4-DBD
    Expression
    Mammalian
    Mutation
    D278K
    Promoter
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only

  28. pLV Rnf121-V5

    Plasmid
    #175130
    Purpose
    Lentiviral expression of mouse Rnf121-V5
    Depositor
    Larry Gerace
    Insert
    Rnf121 (Rnf121 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175130-plasmid-map-sequence-id-352014

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  29. pLV V5-Mospd1

    Plasmid
    #175132
    Purpose
    Lentiviral expression of V5-tagged mouse Mospd1
    Depositor
    Larry Gerace
    Insert
    Mospd1 (Mospd1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175132-plasmid-map-sequence-id-352019

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  30. pSBbi-pur H-2Kb

    Plasmid
    #111623
    Purpose
    Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele
    Depositor
    Jon Yewdell
    Insert
    H2Kb
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111623-plasmid-map-sequence-id-213220

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  31. CROP-seq-opti-dsRed

    Plasmid
    #201999
    Purpose
    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.
    Depositor
    Daniel Geschwind
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    dsRed
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    201999-plasmid-map-sequence-id-398468

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  33. pDY0551-Luciferase RADARS

    Plasmid
    #182538
    Purpose
    Mammalian cell expression plasmid of Gaussia luciferase RADARS in a dual luciferase expression back bone with Cypridina luciferase as the control gene.
    Depositor
    Jonathan Gootenberg, Omar Abudayyeh
    Insert
    RADARS Luciferase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182538-plasmid-map-sequence-id-356645

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  34. pLV V5-Nesprin-3

    Plasmid
    #175136
    Purpose
    Lentiviral expression of V5-tagged mouse Syne3
    Depositor
    Larry Gerace
    Insert
    Syne3 (Syne3 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175136-plasmid-map-sequence-id-352023

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  35. pLV Nup62-V5

    Plasmid
    #175116
    Purpose
    Lentiviral expression of mouse Nup62-V5
    Depositor
    Larry Gerace
    Insert
    Nup62 (Nup62 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175116-plasmid-map-sequence-id-351797

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  36. pLV Tor1a-V5

    Plasmid
    #175135
    Purpose
    Lentiviral expression of Tor1a-Myc
    Depositor
    Larry Gerace
    Insert
    Tor1a (Tor1a Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175135-plasmid-map-sequence-id-352022

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  37. pMC6-TEFkFLP(LYS2)

    Plasmid
    #49458
    Purpose
    overespression of Flp step-arrest mutant in yeast, LYS2 selection
    Depositor
    Marc Gartenberg
    Insert
    Flp(H305L) (flp Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    H305L, ‘step-arrest’ mutant,
    Promoter
    TEF1
    Available since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    49458-plasmid-map-sequence-id-73670

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  38. pLV Nup88-V5

    Plasmid
    #175117
    Purpose
    Lentiviral expression of mouse Nup88-V5
    Depositor
    Larry Gerace
    Insert
    Nup88 (Nup88 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175117-plasmid-map-sequence-id-351804

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  39. pLV Tyw1-V5

    Plasmid
    #175131
    Purpose
    Lentiviral expression of mouse Tyw1-V5
    Depositor
    Larry Gerace
    Insert
    Tyw1 (Tyw1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175131-plasmid-map-sequence-id-352017

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  40. pLV Rbmxl1-V5

    Plasmid
    #175124
    Purpose
    Lentiviral expression of mouse Rbmxl1-V5
    Depositor
    Larry Gerace
    Insert
    Rbmxl1 (Rbmxl1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    175124-plasmid-map-sequence-id-351936

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  41. lenti-RfxCas13d-Blast-BFP

    Plasmid
    #196726
    Purpose
    Sortable and selectable RfxCas13d.
    Depositor
    Bernhard Schmierer
    Insert
    RfxCas13d-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196726-plasmid-map-sequence-id-389737

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  42. lenti.Cas9.BFP.Blast

    Plasmid
    #196714
    Purpose
    WT SpCas9 with BSD-TagBFP for selection and sorting
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196714-plasmid-map-sequence-id-388043

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  43. pLV V5-Sun2

    Plasmid
    #175114
    Purpose
    Lentiviral expression of V5-tagged mouse Sun2
    Depositor
    Larry Gerace
    Insert
    Sun2 (Sun2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175114-plasmid-map-sequence-id-351772

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  44. pAAV_Efla_FLEX_APEX2-eGFPf

    Plasmid
    #171939
    Purpose
    Expresses a membrane targeted (farnesylation) APEX2-eGFP fusion protein
    Depositor
    David Berson
    Insert
    APEX2-eGFPf
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171939-plasmid-map-sequence-id-338504

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  45. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  46. pSBbi-Pur-AktAR2

    Plasmid
    #125195
    Purpose
    SB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    AktAR2
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125195-plasmid-map-sequence-id-243600

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  47. pLV Tmx1-V5

    Plasmid
    #175138
    Purpose
    Lentiviral expression of V5-tagged mouse Tmx1
    Depositor
    Larry Gerace
    Insert
    Tmx1 (Tmx1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175138-plasmid-map-sequence-id-352052

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  48. pLV Tmem9b-V5

    Plasmid
    #175152
    Purpose
    Lentiviral expression of mouse Tmem9b-V5
    Depositor
    Larry Gerace
    Insert
    Tmem9b (Tmem9b Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    175152-plasmid-map-sequence-id-352193

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  49. pLV Ndc1-V5

    Plasmid
    #175115
    Purpose
    Lentiviral expression of mouse Ndc1-V5
    Depositor
    Larry Gerace
    Insert
    Ndc1 (Ndc1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175115-plasmid-map-sequence-id-351773

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  50. pSBbi-Puro_LPAR_AKAR-WT

    Plasmid
    #125206
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-WT
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125206-plasmid-map-sequence-id-243604

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Showing: 2901 - 2950 of 8580 results