We narrowed to 43,776 results for: tra
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Plasmid#174643PurposeOptogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transportDepositorInsertSSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)
TagsSSPB(nano)-GFPExpressionMammalianMutationEGFP: Met1Del; ppKin14VIb(aa861-1321)PromoterChicken beta-actinAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP1A-WT-puro
Plasmid#156166PurposeLentiviral vector for expression of RAP1A-WT, with puromycin selectionDepositorInsertRAP1A (RAP1A Human)
UseLentiviralTagsnoneExpressionMammalianMutationwild type formPromoterhuman PGKAvailable SinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]
Plasmid#159811PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIE-TGFB2-H6
Plasmid#128500PurposeExpresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.DepositorAvailable SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL680 CHYRON3 integration construct
Plasmid#126448PurposeTo integrate the CHYRON3 locus at AAVS1 in human cells.DepositorInsertspU6/3xLacO-CHYRON3 hgRNA
pCMV-puro
ExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6/3xLacOAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
TNPO1-shRNA
Plasmid#86082PurposeA lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.DepositorAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDH3
Plasmid#83789PurposeUseful for tagging yeast proteins with C-terminal CFP tag; G418 selectionDepositorInsertCFP
TagsCFPExpressionYeastMutationF64L, Y66W, N146I, M153T, V163A, N164H mutations …Available SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTF275 (pFA6a-TEV-6xGly-2xStrep-His3MX)
Plasmid#44070DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…Available SinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTH353-SUP45wt
Plasmid#29371DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
ExpressionYeastMutationWild type gene including genomic sequence 1432 bp…Available SinceMay 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pB80-KIF1A(1-365)-VVD52C-GFP-SSPB(micro)
Plasmid#174630PurposePoorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)DepositorInsertKIF1A(1-365)-VVD52C-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
TagsGFP-SSPB(micro)ExpressionMammalianMutationmmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…PromoterChicken beta-actinAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1Tt
Plasmid#44509DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mCherry-2A-BRD4 Iso AdelCTD
Plasmid#137722PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)DepositorInsertBRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationTruncated at amino acid 1328PromoterTight TRE promoterAvailable SinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ashL344A-mCerulean
Plasmid#69580PurposeExpresses p53-L344A mutant tagged with mCeruleanDepositorInsertp53-L344A (TP53 Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutationp53 L344A mutation that prevents tetramerization …PromoterEF1alphaAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAdCMV/V5-DEST-Y705F-STAT3-3xFlag
Plasmid#99261PurposeAdenoviral vector encoding Flag-tagged murine STAT3 (Y705F)DepositorInsertSignal transducer and activator of transcription 3 - Y705F (Stat3 Mouse)
UseAdenoviralTags3x-FlagMutationY705F (contains a silent mutation: nucleotide 271…PromoterCMVAvailable SinceJan. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron(Neo)-G(LNL)
Plasmid#36908DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Neo
ExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_1-560 USP7
Plasmid#131244PurposeMammalian expression of N-terminally Myc-tagged USP7, minus the UBL domainsDepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationExpresses USP7 amino acids 1 - 560. This truncati…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1TCbt
Plasmid#44515DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable SinceApril 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTH732-2µ-RLuc/staCFLuc
Plasmid#40607DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3Available SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDCAF3-IS5
Plasmid#65220PurposeExpresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.DepositorInsertsndmA
ndmB
ndmC
ndmD
gst9
chlR
UseSynthetic BiologyExpressionBacterialMutationChanged start codon from gtg to atg and Changed s…PromoterBBa_J23100Available SinceJan. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZE21/UBP1/ClpS
Plasmid#98566PurposeExpresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operonDepositorInsertsTruncated ubiquitin cleavase, codon optimized
ATP-dependent Clp protease adapter protein
ExpressionBacterialMutationIn a synthetic operon downstream of UBP1 and Remo…PromoterSame as UBP1 (operon) and Tet promoter (aTc induc…Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only