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Showing: 201 - 240 of 1600 results

  4. ROZA-XL YF

    Plasmid
    #64195
    Purpose
    Fluorescent reporter for ZAP-70 tyrosine kinase activity- Y to F mutated control sequence
    Depositor
    Annemarie Lellouch
    Article
    Cadra et al Biochem Biophys Res Commun. 2015 Feb 28.
    Insert
    ROZA-XL YF
    Use
    Tags
    Expression
    Mammalian
    Mutation
    ROZA-XL tyrosine phosphorylation site mutated to …
    Promoter
    CMV
    Available since
    April 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pLL3.7-EF-EYFP-YAP1_Y3F-PolyA

    Plasmid
    #112288
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) Y3F mutant _ tyrosines 341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in this isoform
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    3 Tyrosines (341, 357 and 394 corresponding to ty…
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112288-plasmid-map-sequence-id-213531

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  15. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Article
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Promoter
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60847-plasmid-map-sequence-id-197511

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  16. N295A (EcN), Y160A (EcI) MetNIQ in pBAD

    Plasmid
    #118260
    Purpose
    N295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (Y160A) (metI E. coli)
    MetQ (metQ E. coli)
    Use
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118260-plasmid-map-sequence-id-228496

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  17. A1-LCD Patchy

    Plasmid
    #169719
    Purpose
    Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids.
    Depositor
    Tanja Mittag
    Article
    Martin et al Science. 2020 Feb 7;367(6478):694-699. d
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    hnRNPA1-A Low complexity domain including amino a…
    Promoter
    T7
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169719-plasmid-map-sequence-id-334790

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  18. YARS2_pNIC-CTB10H

    Plasmid
    #153060
    Purpose
    Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase 2
    Depositor
    Susanne Gräslund
    Insert
    YARS2 (YARS2 Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153060-plasmid-map-sequence-id-304480

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  19. pMSP1D12W/2Y

    Plasmid
    #124275
    Purpose
    Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence
    Depositor
    Alexey S. Ladokhin
    Insert
    Membrane scaffold protein
    Use
    Tags
    7xHis-tag
    Expression
    Bacterial
    Mutation
    Two tryptophans changed to two tyrosines
    Promoter
    T7
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    124275-plasmid-map-sequence-id-242402

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  20. pDEST-GST-hSYK

    Plasmid
    #211892
    Purpose
    Express human SYK in mammalian cells
    Depositor
    Haian Fu
    Insert
    SYK (SYK Human)
    Use
    Tags
    GST tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    211892-plasmid-map-sequence-id-419133

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  39. pCMV-myc-FRNK

    Plasmid
    #74481
    Purpose
    This plasmid was constructed in order to characterize the function of Proline-rich tyrosine kinase 2 (PYK2).
    Depositor
    J Thomas Parsons
    Article
    Xiong et al J Cell Biol. 1997 Oct 20;139(2):529-39.
    Insert
    FRNK (PTK2 Chicken)
    Use
    Tags
    c-myc
    Expression
    Mammalian
    Mutation
    FRNK is the C-terminal, noncatalytic domain of th…
    Promoter
    CMV
    Available since
    Aug. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 201 - 240 of 1600 results