We narrowed to 5,868 results for: nls

1. pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

   Plasmid

   #127362

   Purpose

   gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NLS, V5, and YFP

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-TRE3G-NLS-tdMCP-mCherry

   Plasmid

   #99909

   Purpose

   For the expression of NLS-tdMCP-mCherry

   Depositor

   Yujie Sun

   Article

   Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

   Insert

   NLS-tdMCP-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Prrx1:TFPnls-T2a-Cre-Ert

   Plasmid

   #111150

   Purpose

   Limb connective tissue specific inducible Cre

   Depositor

   Elly Tanaka

   Insert

   TFPnls-T2a-Cre-Ert
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   wt

   Promoter

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG1280 NLS-mCherry-FIREtag-NES

   Plasmid

   #205172

   Purpose

   expresses NLS-mCherry-FIREtag-NES in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   NLS-mCherry-FIREtag-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-TRE3G-NLS-tdPCP-EGFP

   Plasmid

   #99910

   Purpose

   For the expression of NLS-tPMCP-EGFP

   Depositor

   Yujie Sun

   Article

   Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

   Insert

   NLS-tdPCP-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE4G

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPyCAG-tagBFP-3xNLS-IRES-Hph

   Plasmid

   #183608

   Purpose

   Mammalian expression vector for tagBFP-3xNLS from the CAG promoter. Confers hygromycin B resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   tagBFP-3xNLS
   

   Use

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLIK 3XFLAG-EGFP-NLS hygro

   Plasmid

   #87781

   Purpose

   Inducible expression of EGFP with a C-terminal NLS sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

   Plasmid

   #199252

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   High specificity eSpCas9(1.1)-D10A nickase
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A K848A K1003A R1060A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-NLS-CBGreen-FIRE-puro

   Plasmid

   #139195

   Purpose

   Luminescent reporter of ERK activity

   Depositor

   Matthew Lazzara

   Article

   Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

   Insert

   CBG99
   

   Use

   Luciferase and Retroviral

   Tags

   Fra1 PEST domain fragment (FIRE) and nuclear loca…

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_EF1a-->Gss52SR4-LaM4-P2A-NLSIFP

    Plasmid

    #162229

    Purpose

    Lentiviral vector for the expression of Gss52SR4-LaM4-P2A-NLSIFP in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    LaG17 GFP nanobody SynNotch tTA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. SBE-NLS-mCherry PGK-rtTA3

    Plasmid

    #195332

    Purpose

    Lentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Inserts

    NLS-mCherry
    rtTA3
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and miniCMV

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-NES-DEVD-mCardinal-NLS

    Plasmid

    #164052

    Purpose

    Fluorescent reporter for caspase-3

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NES-mCardinal-NLS
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Gad65P-eGFP-3XNLS-WPRE-pA

    Plasmid

    #183804

    Purpose

    AAV vector to express nuclear localized eGFP from a Gad65 promoter

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

    Insert

    eGFP-NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS

    Plasmid

    #113436

    Purpose

    Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    type II-C anti-CRISPR
    

    Use

    CRISPR

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)-myc-OGA(GS-544)C181_NLS

    Plasmid

    #193997

    Purpose

    plasmid encoding myc-tagged OGA(GS-544)C181_NLS

    Depositor

    Christina Woo

    Article

    Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

    Insert

    myc-OGA(GS-544)C181_NLS
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. ZIF-NLS-EGFP(Y66F)-HOTag6

    Plasmid

    #208749

    Purpose

    part B of nls-SparkDrop with non-fluorescent eGFP

    Depositor

    Xiaokun Shu

    Article

    Chung et al ACS Cent Sci. 2023 Jul 7;9(7):1466-1479.

    Insert

    ZIF-NLS-EGFP(Y66F)-HOTag6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZac2.1 SV40-CMV-SaCas9-3xNLS

    Plasmid

    #78601

    Purpose

    AAV vector containing SaCas9

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    SV40-CMV-SaCas9-3xNLS
    

    Use

    AAV and CRISPR

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    SV40, CMV promoters

    Available since

    Dec. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pGinB-8X(GGS)-dCas9-FLAG-NLS

    Plasmid

    #81205

    Purpose

    CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    pGinB-8X(GGS)-dCas9-FLAG-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.2/NS5-YFP-NLS-mut

    Plasmid

    #120832

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    ZIKV NS5-eYFP "NLS-mut"
    

    Use

    Tags

    eYFP-V5

    Expression

    Mammalian

    Mutation

    R372A/Q373A/K387A/H388A/K389A

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. SIV URA PCP-NLS-GFPEnvy

    Plasmid

    #189941

    Purpose

    Single-integration vector for inserting PCP-NLS-GFPEnvy at the URA3 locus in Saccharomyces cerevisiae

    Depositor

    Tineke Lenstra

    Article

    Brouwer et al Nat Struct Mol Biol. 2023 May;30(5):692-

    Insert

    PCP-NLS-GFPEnvy
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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