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pCS2-UTX-F

Plasmid

#17438

Purpose

Expression of UTX in mammalian cells

Depositor

Kai Ge

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

Insert

UTX (KDM6A Human)

Tags

6xMyc and Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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1098 pcDNA GFP FKHR

Plasmid

#9022

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR (FOXO1 Human)

Tags

GFP

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#138149

Purpose

Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NLS-HA

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pE-FLP

Plasmid

#45978

Purpose

Expresses yeast Flp driven by the constitutive promoter pE from phage P2

Depositor

Drew Endy, Keith Shearwin

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Insert

flp

Use

Synthetic Biology

Expression

Bacterial

Mutation

flp driven by the constitutive promoter pE from p…

Promoter

pE (from phage P2)

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pCXB-EBNA1

Plasmid

#41857

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

EBNA-1 (EBNA-1 human herpesvirus 4)

Expression

Mammalian

Promoter

CAG

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2

Plasmid

#40978

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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LV-Cre pLKO.1

Plasmid

#25997

Purpose

Lentiviral expression of nls-Cre from the CMV promoter

Depositor

Elaine Fuchs

Article

Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

Insert

Cre recombinase

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pBABE BRG1

Plasmid

#1959

Depositor

Robert Kingston

Article

Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

Insert

BRG1 (SMARCA4 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

E1262K R1575Q

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro p53 shRNA 1

Plasmid

#10671

Depositor

William Hahn

Article

Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

Insert

p53 shRNA (TP53 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pLX313-Firefly luciferase

Plasmid

#118017

Purpose

Constitutive expression of Firefly luciferase

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Firefly luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_pGK_LaG17_synNotch_Gal4VP64

Plasmid

#79127

Purpose

Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

LaG17_synNotch_Gal4DBDVP64

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/pCMV-7.1

Plasmid

#47948

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

Insert

3xFLAG-dCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A (catalyticall…

Promoter

CMV

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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gRNA_AAVS1-T1

Plasmid

#41817

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_AAVS1-T1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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7TFC

Plasmid

#24307

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

7xTcf-FFluc // SV40-mCherry

Use

Lentiviral

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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VSV.G

Plasmid

#14888

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

VSV-G Envelope

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Neo-PIP-FUCCI

Plasmid

#118616

Purpose

Lentiviral expression of the cell cycle marker PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Lentiviral

Tags

mCherry and mVenus

Expression

Mammalian

Promoter

hPGK

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBAV1K-T5-gfp

Plasmid

#26702

Depositor

Ichiro Matsumura

Article

Bryksin et al PLoS One. 2010 . 5(10):e13244.

Insert

T5lacOlacOeGFP biobrick

Expression

Bacterial

Available Since

Nov. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti PGK GFP Puro (w509-5)

Plasmid

#19070

Purpose

3rd gen lentiviral eGFP expression vector, PGK promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

enhanced green fluorescent protein

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pAMPK alpha2 WT

Plasmid

#15991

Depositor

Morris Birnbaum

Article

Mu et al Mol Cell. 2001 May . 7(5):1085-94.

Insert

AMPK alpha2 (Prkaa2 Rat)

Tags

Myc

Expression

Mammalian

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42(T17N)

Plasmid

#12601

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

EGFP-Cdc42 (T17N) (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T17N

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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