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Plasmid#164973PurposeLentiviral vector for NPC1 expression in mammalian cellsDepositorInsertNPC1 (NPC1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationP691SPromoterEF1aAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NPC1(F503/4A)-FLAG
Plasmid#164974PurposeLentiviral vector for NPC1 expression in mammalian cellsDepositorInsertNPC1 (NPC1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationF503A and F504APromoterEF1aAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-Kif5b-Myc
Plasmid#186614PurposeThird generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B fused to Myc tagDepositorInsertKinesin Heavy Chain isoform 5b (KIF5B Human)
UseLentiviralTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NPC1(P202A/F203A)-FLAG
Plasmid#164976PurposeLentiviral vector for NPC1 expression in mammalian cellsDepositorInsertNPC1 (NPC1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationP202A/ F203APromoterEF1aAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT (PGK) 5xGRE-NanoLuc
Plasmid#207170PurposeGAl4 response element driven NanoLuc Lentiviral reporterDepositorInsertsNanoLuciferase
d2eGFP
5x GRE
UseLentiviral, Luciferase, and Synthetic BiologyTags3x SV40 NLS and PESTExpressionMutationPromoterGal4 response element minP and PGKAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-RAB10-WPRE-UbC-Emerald
Plasmid#203804PurposeLentiviral vector plasmid expressing human RAB10 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-SLC35B2-FLAG
Plasmid#154863PurposeTransient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2DepositorInsertSLC35B2 (SLC35B2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationctcctggtgcagtacttc => ttattagtccaatattttPromoterCMVAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
PGK-HA-PGRN
Plasmid#234872PurposeLentiviral plasmid expressing HA-tagged human progranulin.DepositorInsertProgranulin (GRN Human)
UseLentiviralTagsHA tagExpressionMammalianMutationPromoterPGKAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-Emerald
Plasmid#214468PurposeLentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationS14D S25D S32D T261DPromoterAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_19kDa_EE
Plasmid#74951PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsGlu-Glu tag (EYMPME)ExpressionMammalianMutationdelta 1-127PromoterCMV, lentiviral LTRAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_23kDa_Myc
Plasmid#74949PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsHA Tag (YPYDVPDYA)ExpressionMammalianMutationdelta 1-99 M128APromoterCMV, Lentiviral LTRAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_25kDa_Myc
Plasmid#74954PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTags3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)ExpressionMammalianMutationdelta 1-77, M100A M128APromoterCMV, Lentiviral LTRAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUP95Q15ADSKGW (D7)
Plasmid#74030Purposelentiviral expression of Psd95-Q15A deltaSK-EGFP fusionDepositorInsertPsd95 (DLG4 Rat)
UseLentiviralTagsExpressionMammalianMutationQ15A, delta SH3-GK (deletion of the fragment betw…PromoterhUbCAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-PTDSS1-IRES-Puromycin
Plasmid#134667PurposeExpresses EGFP-tagged Mitochondria-associated ER membrane markerDepositorInsertPTDSS1 (PTDSS1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM215A
Plasmid#173217PurposeExpresses ScFV-GCN4-sFGFP10M2-GB1DepositorInsertScFV-GCN4-sfGFP10M2-GB1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM214
Plasmid#173216PurposeExpresses MCP-mCherry-GFP11-GB1DepositorInsertMCP-mCherry-GFP11-GB1
UseCRISPR and LentiviralTagsHA and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
β2AR-PPI-CALPon1.0 (APEX)
Plasmid#213406PurposePeroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 1)DepositorInsertβ2AR-E5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-E-cadherin-RFP
Plasmid#79603PurposepHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging systemDepositorInsertCDH1 promoter region (CDH1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER dCas9-TET1CD
Plasmid#101921PurposeThe dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).DepositorInsertdCas9-TET1CD
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (Sce)
Plasmid#45576PurposeCodes for the SSA-TLR with the target site for I-Sce I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with I-Sce I TS
+3 mCherry
3' iRFP arm
UseLentiviralTagsExpressionBacterial and MammalianMutationembedded I-Sce I TS from 163-185bp, truncated 25 …PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-Kif5c-Myc
Plasmid#186616PurposeThird generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform C fused to Myc tagDepositorInsertKinesin Heavy Chain isoform 5c (KIF5C Human)
UseLentiviralTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Kif5a-Myc
Plasmid#186613PurposeThird generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform A fused to Myc tagDepositorInsertKinesin Heavy Chain isoform 5A (KIF5A Human)
UseLentiviralTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
TurboID-CRaf
Plasmid#226168PurposeLentiviral plasmid used to express a TurboID fusion of the protein kinase CRaf.DepositorInsertMyc-TurboID-CRaf (RAF1 Human)
UseLentiviralTagsEGFP-IRES-BlastR and Myc-TurboIDExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-RAB10 Q68L-WPRE-UbC-Emerald
Plasmid#203803PurposeLentiviral vector plasmid expressing human RAB10 mutation Q68L (constitutively active mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationQ68LPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NPC1(D786N)-FLAG
Plasmid#164975PurposeLentiviral vector for NPC1 expression in mammalian cellsDepositorInsertNPC1 (NPC1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationD786N, insertion L aa15 (please see depositor co…PromoterEF1aAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-RAB10 T23N-WPRE-UbC-Emerald
Plasmid#203802PurposeLentiviral vector plasmid expressing human RAB10 mutation T23N (dominant negative mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationT23NPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (Ani)
Plasmid#45577PurposeCodes for the SSA-TLR with the target site for I-Ani I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with I-Ani I TS
+3 mCherry
3' iRFP arm
UseLentiviralTagsExpressionBacterial and MammalianMutationembedded I-Ani I TS from 163-185bp, truncated 25 …PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-Kif5b-T92N-Myc
Plasmid#186615PurposeThird generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B Rigor mutant (T92N) fused to Myc tagDepositorInsertKinesin Heavy Chain isoform 5b Rigor mutant (T92N) (KIF5B Human)
UseLentiviralTagsMyc tagExpressionMammalianMutationT92NPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_26kDa_Myc
Plasmid#74955PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 26kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsStrep II Tag (GGGGWSHPQFEKGGGGWSHPQFEK)ExpressionMammalianMutationdelta (1-62) M78A M80A M100A M128APromoterCMV, Lentiviral LTRAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_33kda_Myc
Plasmid#74957PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 33kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsMyc tag (EQKLISEEDL)ExpressionMammalianMutationM63A M78A M80A M100A M128APromoterCMV, Lentiviral LTRAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
HIVGKO
Plasmid#112234PurposeHIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1aDepositorInsertcsGFP-EF1a-mKO2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBA409
Plasmid#85971PurposeEF1a-sfGFP-Ubc-NeoDepositorInsertssfGFP
Neo
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
ScFv-2ERT2-V
Plasmid#120553Purpose3rd gen transfer vector. Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP, 2 tandem ERT2 and VP64.DepositorInsertscFvGCN4, sfGFP, GB1, ERT2, VP64
UseLentiviralTagsExpressionMammalianMutationPromoterhPGKAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPKm-243
Plasmid#90506PurposemOrange and mitochondrial-tagged sfGFP (pSIN-OFP-mitosfGFP)DepositorInsertmOrange
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pRS140
Plasmid#197141PurposescCloneSelect C-to-T reporter encoding BC-C1 barcode (CMV promoter version)DepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterCMVAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS191
Plasmid#197140PurposescCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter version)DepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterEF1aAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-CS-117
Plasmid#197134PurposeCloneSelect C-to-T reporter encoding three target barcodes (3-input OR gate circuit)DepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-CS-119
Plasmid#197135PurposeCloneSelect A-to-G reporter encoding three target barcodes (3-input AND gate circuit)DepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR85
Plasmid#140095PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRLsin-SV40 T antigen-IRES-mCherry
Plasmid#58993Purposelentiviral expression of SV40 large T antigen and mCherry red fluorescent reporterDepositorInsertsCMV enhancer-chicken beta actin promoter
SV40 large T antigen
IRES-mCherry red fluorescent protein
UseLentiviralTagsExpressionMammalianMutationEcoRV site in MCS converted to BamHI sitePromoterCMV enhancer-chicken beta actinAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-CKAP4-WPRE-UbC-Emerald
Plasmid#225943PurposeLentiviral vector plasmid expressing human cytoskeleton associated protein 4 (CKAP4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterSFFV and UbCAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER dCas9-TET1CDMut
Plasmid#101920PurposeThe dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.DepositorInsertdCas9-TET1CD Mut
UseLentiviralTagsExpressionMammalianMutationMutated TET1 catalytic domainPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0-Anillin shRNA
Plasmid#187270PurposeLentiviral expression of shRNA targeting AnillinDepositorInsertshRNA for Anillin (ANLN Human)
UseLentiviralTagsEGFPExpressionMutationPromoterU6Available sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (CCR5 TALEN Sce Spacer)
Plasmid#46941PurposeCodes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with TALEN TS
+3 mCherry
3' iRFP arm
UseLentiviralTagsExpressionBacterial and MammalianMutationembedded TALEN TS from 163-218, truncated 25 amin…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-RTN3-WPRE-UbC-Emerald
Plasmid#233467PurposeLentiviral vector plasmid expressing human reticulon 3 (RTN3) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterSFFV and UbCAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-myc-ATL3-WPRE-UbC-Emerald
Plasmid#225946PurposeLentiviral vector plasmid expressing human atlastin GTPase 3 (ATL3) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsmyc-tagExpressionMammalianMutationPromoterSFFV and UbCAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-CALU-WPRE-UbC-Emerald
Plasmid#225951PurposeLentiviral vector plasmid expressing human calumenin (CALU) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterSFFV and UbCAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only