166,169 results

1. ABE8.20-m

   Plasmid

   #136300

   Purpose

   expresses ABE8.20-m in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.20-m
   

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3-N90-beta-catenin

   Plasmid

   #31785

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   beta-catenin (CTNNB1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deletion of aa 1-90- Constitutitvely Active

   Promoter

   EF1alpha

   Available Since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. HER2 WT

   Plasmid

   #16257

   Depositor

   Mien-Chie Hung

   Article

   Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

   Insert

   HER2 (ERBB2 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-Tet3G blasticidin

   Plasmid

   #128061

   Purpose

   Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDD162 (Peft-3::Cas9 + Empty sgRNA)

   Plasmid

   #47549

   Purpose

   Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Inserts

   Cas9
   Empty sgRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Promoter

   U6 and eef-1A.1 (eft-3)

   Available Since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-CreERT2

   Plasmid

   #14797

   Purpose

   Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

   Plasmid

   #168290

   Purpose

   CRISPR-activator Suntag-P65-HSF1 (SPH)

   Depositor

   Hui Yang

   Article

   Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

   Insert

   dCas9, Suntag-P65-HSF1
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-mCherry-flex-dtA

   Plasmid

   #58536

   Purpose

   Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

   Depositor

   Naoshige Uchida

   Article

   Wu et al Nature. 2014 May 15;509(7500):325-30. do

   Insert

   dtA (DIP_RS18250 )
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS U3-hTR-500

   Plasmid

   #28170

   Depositor

   Kathleen Collins

   Article

   Fu et al Mol Cell. 2003 May . 11(5):1361-72.

   Insert

   U3-hTR-500 (TERC Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC013 - Twinstrep-SUMO-huLwCas13a

    Plasmid

    #90097

    Purpose

    Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a
    

    Use

    CRISPR

    Tags

    6xHis-Twin Strep-SUMO

    Expression

    Bacterial

    Available Since

    June 13, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pLv-HSA-uDys/eGFP

    Plasmid

    #26810

    Depositor

    Jeffrey Chamberlain

    Article

    Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

    Insert

    dystrophin (DMD Human)
    

    Use

    Lentiviral; Lentiviral plasmid backbone from l. n…

    Tags

    eGFP

    Mutation

    deleted R4-R23 deleted exons 71-78

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. M51 Super 8x FOPFlash (TOPFlash mutant)

    Plasmid

    #12457

    Purpose

    Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

    Depositor

    Randall Moon

    Article

    Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

    Insert

    mutant TCF/LEF binding sites (Ctnnb1 )
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    mutant TCF/LEF binding sites

    Available Since

    March 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

    Plasmid

    #207664

    Purpose

    Neuropeptide degradation

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    NEP (MME Human)
    

    Use

    AAV and Cre/Lox

    Tags

    mRUBY3

    Mutation

    Addition of signaling peptide (POMC) on the N-ter…

    Promoter

    hSynapsin

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-mRFP

    Plasmid

    #13032

    Purpose

    Mammalian expression vector for cloning your gene fused to mRFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Insert

    Monomer Red Fluorescent Protein
    

    Expression

    Mammalian

    Available Since

    Nov. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. SBE4-Luc

    Plasmid

    #16495

    Purpose

    Luciferase reporter containing four copies of the Smad binding site

    Depositor

    Bert Vogelstein

    Article

    Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

    Insert

    Smad binding element
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEMS-CV SUy-Halo7Tag

    Plasmid

    #108933

    Purpose

    Selflabeling tag at ATP Synthase

    Depositor

    Karin Busch

    Article

    Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

    Insert

    ATP synthase F1 subunit gamma (ATP5F1C Human)
    

    Tags

    Halo7 tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-EGFP (BPK1098)

    Plasmid

    #133962

    Purpose

    CMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcription

    Depositor

    Benjamin Kleinstiver

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pORANGE Cacnb3-GFP KI

    Plasmid

    #139662

    Purpose

    Endogenous tagging of CaVβ3: C-terminal (amino acid position: W479)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-YFP

    Plasmid

    #13033

    Purpose

    Mammalian expression vector for cloning your gene fused to YFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Insert

    Yellow fluorescent protein
    

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX303-ZIM3-KRAB-dCas9

    Plasmid

    #154472

    Purpose

    Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Synthetic)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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