161,727 results

1. EGFP-Tau(WT)

   Plasmid

   #187023

   Purpose

   Expresses EGFP tagged human 2N4R tau (WT) in mammalian cells with CMV promoter

   Depositor

   George Bloom

   Article

   Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

   Insert

   MAPT (MAPT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMVR8.74

   Plasmid

   #22036

   Purpose

   2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Insert

   gag pol tat rev
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBMN(CMV-copGFP-Luc2-Puro)

   Plasmid

   #80389

   Purpose

   To make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection marker

   Depositor

   Magnus Essand

   Article

   Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

   Insert

   copGFP-T2A-Luc2-F2A-Puromycin
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57.1_V5_TurboID_FLAG_Nt

   Plasmid

   #194072

   Purpose

   3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5-TurboID-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29b_1_anti-GFP-nanobody

   Plasmid

   #210394

   Purpose

   Express anti-GFP nanobody in E. coli

   Depositor

   Magnus Rueping

   Article

   Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

   Insert

   1_anti-GFP-nanobody
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCRISPR

   Plasmid

   #42875

   Purpose

   A crRNA expression plasmid for targeting a specific sequence.

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   CRISPR-BsaI
   

   Use

   CRISPR; E.coli

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-BAIAP2 wt

   Plasmid

   #31675

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   BAI1-associated protein 2 (BAIAP2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHCMV_Bless

   Plasmid

   #207255

   Purpose

   Mammalian expression of Baboon Endogenous Retrovirus env protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   BaboonEndogenousRetrovirus_Rless_env
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-GRAB_g5-HT3.0

   Plasmid

   #208717

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Has Service

   AAV1 and AAV2

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFGET19_Ulp1

    Plasmid

    #64697

    Purpose

    H6-Ulp1 from pHYRS52 into pET-28b(+)

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Insert

    ULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Ulp1(403-621)

    Promoter

    T7

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-AAVS1

    Plasmid

    #227272

    Purpose

    Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting AAVS1 (AAVS1 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC1-HyPer-Red

    Plasmid

    #48249

    Purpose

    Genetically encoded hydrogen peroxide indicator with red fluorescence.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HYPER-RED
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-SIRT1

    Plasmid

    #1791

    Purpose

    Mammalian expression of flag-tagged SIRT1

    Depositor

    Michael Greenberg

    Article

    Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG_PSD95.FingR-eGFP-CCR5TC

    Plasmid

    #46295

    Depositor

    Don Arnold

    Article

    Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

    Insert

    PSD95.FingR-eGFP-CCR5TC (DLG4 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-TRE-dCas9-VPR

    Plasmid

    #63800

    Purpose

    SP-dCas9-VPR with doxycycline-inducible expression

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CAG.iGluSnFR3.v857.GPI

    Plasmid

    #178335

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmScarlet3-Giantin_C1

    Plasmid

    #189773

    Purpose

    Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-Giantin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-EF1-mVenus-p27K−

    Plasmid

    #176651

    Purpose

    It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).

    Depositor

    Mohamed Bentires-Alj

    Article

    Correia et al Nature. 2021 Jun;594(7864):566-571. doi:

    Insert

    mVenus-p27K- (Cdkn1b Mouse)
    

    Use

    Lentiviral

    Tags

    mVenus

    Expression

    Mutation

    Mutant K- (lacks affinity to Cdk)

    Promoter

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

    Plasmid

    #180263

    Purpose

    Lentiviral expression vector for dCas9-VP64 in mammalian cells

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Nuclease dead Cas9

    Promoter

    SFFV

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-GRAB-gDA3m

    Plasmid

    #208698

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Has Service

    AAV1

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_gDA3m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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