We narrowed to 17,223 results for: Emb;
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Plasmid#75228PurposeAn adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor GammohDepositorAvailable SinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only
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pCMMP-NGFR:LMP1-IRES-mRFP
Plasmid#36974DepositorUseRetroviralExpressionMammalianMutationNGFR:LMP1 chimeric receptor consists of aa 1–276 …PromoterCMVAvailable SinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH744-CEN-RLuc/slowmaxCFLuc
Plasmid#38224DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKK-mVenus-TEV
Plasmid#105774PurposeExpression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsmVenus-TEVExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH742-CEN-RLuc/slowminCFLuc
Plasmid#38222DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3 (=GPD)Available SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
FSW TMEM192-3xHA
Plasmid#246729PurposeNeuron-specific expression of human TMEM192 with a tag of 3x HA on its C-terminusDepositorInsertTMEM192 (transmembrane protein 192) (TMEM192 Human)
UseLentiviralTags3xHAExpressionMammalianPromoterSynapsinAvailable SinceDec. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-DV-IRES-myr-EGFP (JDW 495)
Plasmid#242574PurposeGateway destination vector with a CAGGS promoter in the backbone as well as an IRES-myr-EGFP to label cell membranes.DepositorInsertattR1-CmR-ccdB-attR2
ExpressionMammalianPromoterCAGGSAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-DV-IRES-myr-mKate (JDW 494)
Plasmid#242575PurposeGateway destination vector with a CAGGS promoter in the backbone as well as an IRES-myr-mKate to label cell membranes.DepositorInsertattR1-CmR-ccdB-attR2
ExpressionMammalianPromoterCAGGSAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
ER-RspA-NFAST
Plasmid#233595PurposeExpression of RspA-NFAST on the ER membraneDepositorInsertRspA-NFAST
ExpressionMammalianPromoterCMVAvailable SinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
ER-long-RspA-NFAST
Plasmid#233600PurposeExpression of RspA-NFAST on the ER membrane after a 55 aminoacid linkerDepositorInsertRspA-NFAST
ExpressionMammalianPromoterCMVAvailable SinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX4
Plasmid#223340PurposeMapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorInsertABCD1-EGFP-miniTurboID-3HA (PXA1 Mustard Weed)
TagsEGFP-miniTurboID-3HAExpressionPlantPromoterCaMV35SAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX3
Plasmid#223339PurposeMapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorInsertABCD1-miniTurboID-EGFP-3HA (PXA1 Mustard Weed)
TagsminiTurboID-EGFP-3HAExpressionPlantPromoterCaMV35SAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX2
Plasmid#223338PurposeMapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorInsertABCD1-EGFP-TurboID-3HA (PXA1 Mustard Weed)
TagsEGFP-TurboID-3HAExpressionPlantPromoterCaMV35SAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX1
Plasmid#223337PurposeMapping of cytosol-facing peroxisome outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorInsertABCD1-TurboID-EGFP-3HA (PXA1 Mustard Weed)
TagsTurboID-EGFP-3HAExpressionPlantPromoterCaMV35SAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
MT2
Plasmid#223330PurposeMapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
MT3
Plasmid#223331PurposeMapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorInsert3HA-EGFP-miniTurboID-OMP25 (Synj2bp Rat)
Tags3HA-EGFP-miniTurboIDExpressionPlantPromoterCaMV35SAvailable SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
MT4
Plasmid#223332PurposeMapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorInsert3HA-miniTurboID-EGFP-OMP25 (Synj2bp Rat)
Tags3HA-miniTurboID-EGFPExpressionPlantPromoterCaMV35SAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-EZR
Plasmid#227294PurposeDonor template for mStayGold insertion into the C-terminus of the EZR locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-EZR (Addgene #227293)DepositorInsertEZR Homology Arms flanking a mStayGold Tag (EZR Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-myr-mTagBFP2-Flag (JDW 1183)
Plasmid#224492PurposeGateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)DepositorInsertmyr-mTagBFP2-Flag
UseGateway cloningAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_Cas9-hGem_Smc4
Plasmid#217667Purposeexpresses Cas9-hGem and guideRNA for tagging of SMC4DepositorInsertsgRNA1 targeting SMC4 C-terminal (SMC4 Human)
UseCRISPRExpressionBacterial and MammalianPromoterU6Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Hygro-P2A-HA-FKBP-WAPL
Plasmid#217666PurposeRepair template to endogenously tag WAPL with Hygro-P2A-HA-FKBPDepositorInsertWapI (WAPL Human)
TagsdTag (FKBP36V protein)ExpressionBacterial and MammalianPromoterLacAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-L819Y-siRNAres_AC
Plasmid#148394PurposeInsect Expression of DmAGO1-L819Y-siRNAresDepositorInsertDmAGO1-L819Y-siRNAres (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations compared to…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-P714GA744FL819Y-siRNAres_AC
Plasmid#148395PurposeInsect Expression of DmAGO1-P714GA744FL819Y-siRNAresDepositorInsertDmAGO1-P714GA744FL819Y-siRNAres (AGO1 Fly)
ExpressionInsectMutationvariation with 2 silent mutations compared to the…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNAres_AC
Plasmid#148404PurposeInsect Expression of DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNaresDepositorInsertDmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares (Not1 Fly)
ExpressionInsectMutation10 silent mutations and two non silent N605S, K75…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot1iso1_1318-1605-shRNAres_AC
Plasmid#148414PurposeMammalian Expression of HsNot1iso1_1318-1605-shRNAresDepositorInsertHsNot1iso1_1318-1605-shRNAres (CNOT1 Human)
ExpressionMammalianMutation1 silent mutation compared to the sequence of iso…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6A_1-1609-del1384-1524-siRNAres_N
Plasmid#147024PurposeMammalian Expression of HsTNRC6A_1-1609-del1384-1542-siRNAresDepositorInsertHsTNRC6A_1-1609-del1384-1542-siRNAres (TNRC6A Human)
ExpressionMammalianMutationone non silent mutation K1578E compared to the se…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-siRNAres-HA_O
Plasmid#147097PurposeInsect Expression of DmPABPC1-siRNAresDepositorInsertDmPABPC1-siRNAres (pAbp Fly)
ExpressionInsectMutationseven silent mutations compared to the sequence g…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1_1-550-siRNAres_L
Plasmid#146859PurposeInsect Expression of DmPABPC1_1-550-siRNAresDepositorInsertDmPABPC1_1-550-siRNAres (pAbp Fly)
ExpressionInsectMutationone non silent mutation G26S, and six silent muta…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1_90-635-siRNAres-V5His6_L
Plasmid#146860PurposeInsect Expression of DmPABPC1_90-635-siRNAresDepositorInsertDmPABPC1_90-635-siRNAres (pAbp Fly)
ExpressionInsectMutationfive silent mutations compared to the sequence gi…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-DmPABPC1-del91-164-V5His6_L
Plasmid#146861PurposeInsect Expression of DmPABPC1_del91-164DepositorInsertDmPABPC1_del91-164 (pAbp Fly)
ExpressionInsectMutationone non silent mutation G26S, and five silent mut…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182_1-1199-del862-1115-dsRNAres_L
Plasmid#146820PurposeInsect Expression of DmGW182_1-1199-del862-1115-dsRNAresDepositorInsertDmGW182_1-1199-del862-1115-dsRNAres (gw Fly)
ExpressionInsectMutationTwo non silent mutations V704A and N799S and one …Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6A-del1352-1369-siRNAres_K
Plasmid#146743PurposeMammalian Expression of HsTNRC6A-del1352-1369-siRNAresDepositorInsertHsTNRC6A-del1352-1369-siRNAres (TNRC6A Human)
ExpressionMammalianMutationone non silent mutation K1578E compared to the se…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
RPS4X sgRNA-3 lentiCRISPR v2-Blast plasmid
Plasmid#192233Purposelentiviral vector expressing sgRNA-3 targeting eIF3 binding site of RPS4X 5'UTRDepositorInsertsgRNA-3 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
UseCRISPR and LentiviralAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
RPS4X sgRNA-4 lentiCRISPR v2-Blast plasmid
Plasmid#192234Purposelentiviral vector expressing sgRNA-4 targeting eIF3 binding site of RPS4X 5'UTRDepositorInsertsgRNA-4 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
UseCRISPR and LentiviralAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
RPS4X sgRNA-2 lentiCRISPR v2 plasmid
Plasmid#192232Purposelentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTRDepositorInsertsgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
UseCRISPR and LentiviralAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
RPS4X sgRNA-1 lentiCRISPR v2 plasmid
Plasmid#192231Purposelentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS4X 5'UTRDepositorInsertsgRNA-1 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
UseCRISPR and LentiviralAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres_AC
Plasmid#148442PurposeMammalian Expression of HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAresDepositorInsertHsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres (CNOT1 Human)
ExpressionMammalianMutation1 silent mutation compared to the sequence of iso…Available SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-chPKCdelta/eDHFR(69K6)-EGFP
Plasmid#178857PurposeMammalian expression of PKCδ-eDHFR(69K6) chimera fused to EGFPDepositorAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670
Plasmid#178858PurposeMammalian expression of PKCδ-eDHFR(69K6) chimera fused to miRFP670DepositorInsertPKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-miRFP670 (PRKCD Human)
ExpressionMammalianPromoterCAGAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-62-SFFV-T2A-PuroR-polyA-R11b
Plasmid#179893PurposeDonor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)DepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inExpressionBacterial and MammalianPromoterSFFVAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-64-SFFV-T2A-BlaR-polyA-R11b
Plasmid#179895PurposeDonor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)DepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inExpressionBacterial and MammalianPromoterSFFVAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6A_1242-1709-F1359A_K
Plasmid#146742PurposeMammalian Expression of HsTNRC6A_1242-1709-F1359ADepositorInsertHsTNRC6A_1242-1709-F1359A (TNRC6A Human)
ExpressionMammalianMutationfive non silent mutations D1289G, G1515A, S1516G,…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo2 mycBioID-Tollip M240A,F241A
Plasmid#178055Purposemyc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.DepositorInsertToll-Interacting Protein (TOLLIP Human)
TagsmycBioIDExpressionMammalianMutationChanged Methionine 240 to Alanine and changed Phe…PromoterCMVAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_charge_RtoK
Plasmid#139127Purposeexpress FUS LC with hnRNPA2-like charge patterning where all R are changed to KDepositorInsertFUS (FUS Human)
ExpressionBacterialMutationAdd K and D to FUS LC so it has the same charge p…Available SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
FB026
Plasmid#119711PurposeTranscriptional Unit (TU) for YFP expression, obtained by combining FB/GBparts FB007+GB0053+FB008 into pDGB3alpha1R. According to FungalBraid/GoldenBraid modular DNA assembly for ATMTDepositorInsertPromoter gpdA:YFP coding sequence:Terminator trpC
UseSynthetic BiologyAvailable SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mCardinal
Plasmid#105798PurposeExpression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mCardinalExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mCerulean
Plasmid#105790PurposeExpression of your protein of interest in fusion with cyan fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mCeruleanExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mCardinal-TEV
Plasmid#105781PurposeExpression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsmCardinal-TEVExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mCerulean-TEV
Plasmid#105773PurposeExpression of your protein of interest in fusion with cyan fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsmCerulean-TEVExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
KLHL31 pgk (1088)
Plasmid#62031Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only