We narrowed to 1,410 results for: 4-Oct

1. pLKO_HA.AU1.FLAG_NGFR

   Plasmid

   #158236

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   HA.AU1.FLAG

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO_NWS.AU1.V5_NGFR

   Plasmid

   #158237

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   NWS.AU1.V5

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-Hyg-Snrnp40-skywarp-CRISPR-resistant

   Plasmid

   #134252

   Purpose

   Lentivector encoding CRISPR-resistant skywarp form of Snrnp40 (missing exon 5)

   Depositor

   Bruce Beutler

   Article

   Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

   Insert

   Snrp40 (Snrnp40 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   deleted amino acids 179-219, and mutated coding s…

   Promoter

   EF1a

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Sox2-IRES-Neo

   Plasmid

   #13462

   Depositor

   Shinya Yamanaka

   Article

   Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-Stat5a1*6

   Plasmid

   #130668

   Purpose

   Retroviral vector for the expression of constitutive Stat5a (puromycin resistance)

   Depositor

   Gerardo Ferbeyre

   Article

   Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

   Insert

   Stat5a (Stat5a Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Histidine 298 to Arginine and Serine 710 …

   Promoter

   LTR

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCis_2+7+8+5

   Plasmid

   #60589

   Purpose

   a 4-integrase plasmid

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Cis_2+7+8+5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-mWnt3

   Plasmid

   #42276

   Depositor

   Jeremy Nathans, Chris Garcia

   Article

   Yu et al Development. 2012 Dec 1;139(23):4383-94.

   Insert

   Wnt3 (Wnt3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pBAV-A

   Plasmid

   #65927

   Purpose

   Theophylline Riboswitch A with lacZ gene

   Depositor

   Justin Gallivan

   Article

   Topp et al Appl Environ Microbiol. 2010 Dec;76(23):

   Insert

   lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pBAV-B

   Plasmid

   #65928

   Purpose

   Theophylline Riboswitch B with lacZ gene

   Depositor

   Justin Gallivan

   Article

   Topp et al Appl Environ Microbiol. 2010 Dec;76(23):

   Insert

   lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pWZL-Stat5a1*6

    Plasmid

    #130669

    Purpose

    Retroviral vector for the expression of constitutive Stat5a (hygromycin resistance)

    Depositor

    Gerardo Ferbeyre

    Article

    Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

    Insert

    Stat5a (Stat5a Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Histidine 298 to Arginine and Serine 710 …

    Promoter

    LTR

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMaster12

    Plasmid

    #58527

    Purpose

    Reprogram somatic cells to iPS cells

    Depositor

    Mario Capecchi

    Article

    Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

    Inserts

    neo
    tk
    oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    Myc (MYC Human)
    EBNA-1
    Amp
    Lin28 (LIN28A Human)
    NANOG (NANOG Human)
    IRES
    NR5A2 (NR5A2 Human)
    miR302/367 (MIR367, MIR302A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMasterE

    Plasmid

    #165080

    Purpose

    Episomal expression of reprogramming factors.

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 May;53:102380. doi:

    Inserts

    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    MYC (MYC Human)
    LRH1 (NR5A2 Human)
    NANOG (NANOG Human)
    LIN28 (LIN28A Human)
    EGFP
    HSV TK
    

    Use

    Episomal

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMaster3

    Plasmid

    #58526

    Purpose

    Reprogram somatic cells to iPS cells

    Depositor

    Mario Capecchi

    Article

    Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

    Inserts

    neo
    tk
    oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 )
    myc (MYC Human)
    EBNA-1
    Amp
    Lin28 (LIN28A Human)
    Nanog (NANOG Human)
    IRES
    NR5A2 (NR5A2 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-EEA-5g-OSK2M2L1-PGK-Puro

    Plasmid

    #102909

    Purpose

    PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-C6F/TdTomato

    Plasmid

    #140826

    Purpose

    OCT4, SOX2, KLF4, C-MYC, KLF2, NANOG (C6F) and tdTomato under the control of TRE (Tet-ON)

    Depositor

    Hideyuki Okano

    Article

    Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

    Inserts

    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    C-MYC (MYC Human)
    KLF2 (KLF2 Human)
    NANOG (NANOG Human)
    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L104P- please see depositor comments

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcmv3-IRG1-HA

    Plasmid

    #198181

    Purpose

    Mammalian expression of HA-tagged human ACOD1 (IRG1)

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    ACOD1 (ACOD1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    HA tag is added to C-terminal of target protein

    Promoter

    CMV

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLQ10715

    Plasmid

    #183960

    Purpose

    U6-crOct4-CAG-hyperdCas12a-miniVPR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Inserts

    HyperdCas12a
    crRNA targeting Oct4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CAG and U6

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLM-mCerulean-cMyc

    Plasmid

    #23244

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCerulean-2A-MYC (MYC synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLM-mCherry-Klf4

    Plasmid

    #23243

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCherry_2A_KLF4 (KLF4 synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcmv3-IRG1-N152S-HA

    Plasmid

    #198182

    Purpose

    Mammalian expression of HA-tagged human ACOD1 (IRG1) N152S

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    ACOD1 (ACOD1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    The asparagine in IRG1 at position 152 mutates to…

    Promoter

    CMV

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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