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pTXTL-T7max-LuxA
Plasmid#188305PurposeExpression of LuxA via a T7 promoter in TXTLDepositorInsertluxA
UseLuciferase and Synthetic BiologyTagsExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
MIG(F)-RelA
Plasmid#26986DepositorInsertRelA (Rela Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pADtet-His6-MscL
Plasmid#83373PurposeExpresses wild type MscL in mammalian cellsDepositorInsertMechanosensitive channel of large conductance (mscL E. coli)
UseAdenoviralTagsHis6 tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-LMO-IRES-EGFP
Plasmid#178309PurposeExpress the bacterial lactate 2-monooxygenase (LMO) enzyme in mammalian cellsDepositorInsertLactate 2-monooxygenase
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/HuR.wt
Plasmid#110346PurposeDoxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG tagDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromotergagAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
p18S.7 tag
Plasmid#51730Purposetranscribes truncated transcript containing 18S and hybridization tagDepositorInsert18S
UseTagsExpressionMutationPromoterpol-IAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
GWF094
Plasmid#133616PurposeNS3aH1-mCherryDepositorInsertNS3aH1-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
GWF196
Plasmid#133613PurposeNS3aH1-VPRDepositorInsertNS3aH1-VPR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4134900
Plasmid#151911PurposeMammalian expression plasmid for SARS-CoV-2 spike prefusion-stabilizedDepositorInsertSARS-CoV-2 spike prefusion-stabilized (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationd1-15 d1209-1273 R682A R685G K986P V987PPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19-U6-AksgRNA
Plasmid#121955PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertAkCas12b single chimeric gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S2
Plasmid#128590PurposeExpresses tagless S2 ribosomal protein in E.coliDepositorInsert30S ribosomal subunit protein S2 (rpsB E.coli)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
[GG][Pin]
Plasmid#97191PurposePin nuclease domain with Pum cloning siteDepositorInsertPin domain
UseSynthetic BiologyTagsPum cloning siteExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_TEV
Plasmid#58868PurposeMESA protease chain with mCherry ectodomain and no linker domainDepositorInsertMESA protease chain with mCherry ectodomain and TEV protease
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_TEV
Plasmid#58872PurposeMESA protease chain with dTomato ectodomain and no linker domainDepositorInsertMESA protease chain with dTomato ectodomain and TEV protease
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FRBx2
Plasmid#193571PurposeExpresses mCherry tagged with two FRB mutants (T2098L) in mammalian cells.DepositorInsertmCherry
UseTagsFRB (T2098L)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-HA-mAVO3 (pRL62-3)
Plasmid#39210DepositorInsertmAVO3 (Rictor Mouse)
UseTagsHAExpressionMammalianMutationG5S and I15VPromoterCMVAvailable sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-AasgRNA
Plasmid#121954PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertAaCas12b single chimeric gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEntry Tshld-GFP
Plasmid#53211PurposepEntry Tshld-GFP is a product of BP recombination reaction between attB flanked Tshld system-encoded PCR product and pDonor 201 plasmid.DepositorInsertTshld-GFP
UseTagsEGFP, Flag, and HAExpressionMutationPromoternoneAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-RNF40i1
Plasmid#59594PurposeExpression of shRNA against human RNF40DepositorInsertRNF40 (RNF40 Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-TTF2i2
Plasmid#59623PurposeExpression of shRNA against human TTF2DepositorInsertTTF2 (TTF2 Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-TTF2i1
Plasmid#59622PurposeExpression of shRNA against human TTF2DepositorInsertTTF2 (TTF2 Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTYF-EF1a-DLDH-IRES-EGFP
Plasmid#178310PurposeExpress the bacterial D-lactate dehydrogenase (DLDH) enzyme in mammalian cellsDepositorInsertD-lactate dehydrogenase
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-AasgRNA3.8
Plasmid#121958PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertAaCas12b single chimeric gRNA, short version
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
stathmin-CFP
Plasmid#86783PurposeMammalian expression of stathmin-CFPDepositorInsertstathmin (STMN1 Human)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
GWF148
Plasmid#133617PurposeGNCR1-BFP-CAAXDepositorInsertGNCR1-BFP-CAAX
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLH-stsgRNA2.1
Plasmid#64117PurposeVector for expression of St1 sgRNA2.1 in mammalian cellsDepositorInsertSt1 sgRNA2.1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pEGFPC1-Sclt1
Plasmid#128873PurposeFor mammalian expression of mouse Sclt1DepositorInsertSclt1 (Sclt1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-RNF40i2
Plasmid#59595PurposeExpression of shRNA against human RNF40DepositorInsertRNF40 (RNF40 Human)
UseRNAiTagsExpressionMammalianMutationPromoteruAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTAPmyc-T2A-tagRFP
Plasmid#140278PurposeBackbone for making a mammalian expression vector of the protein of interestDepositorInsertEmpty backbone
UseSynthetic BiologyTagsmyc-T2A-tagRFPExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4252845
Plasmid#153787PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHis-003ExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF-hSEMA4C-Fc
Plasmid#74449PurposeExpresses human Semaphorin-4C ectodomain fused to human Fc in mammalian cellsDepositorInserthuman Semaphorin-4C ectodomain with human Fc tag (SEMA4C Human)
UseTagshuman Fc tagExpressionMammalianMutationPromoterEF1-alphaAvailable sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-LuxB
Plasmid#188306PurposeExpression of LuxB via a T7 promoter in TXTLDepositorInsertluxB
UseLuciferase and Synthetic BiologyTagsExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
px459 EQR
Plasmid#101732PurposeExpresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifsDepositorInsertSpCas9 EQR
UseTagsExpressionMammalianMutationVQRPromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLH-stsgRNA1.1
Plasmid#64116PurposeVector for expression of St1 sgRNA1.1 in mammalian cellsDepositorInsertSt1 sgRNA1.1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-HisGFPHiBiT
Plasmid#188316PurposeExpression of GFP-HiBiT via T7 promoter in TXTLDepositorInsertGFP-HiBiT fusion
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-HiBiTGFPHis
Plasmid#188317PurposeExpression of HiBiT-GFP via T7 promoter in TXTLDepositorInsertHiBiT-GFP fusion
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4134904
Plasmid#151930PurposeMammalian expression plasmid for SARS-CoV-2 spike prefusion-stabilizedDepositorInsertSARS-CoV-2 spike prefusion-stabilized (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationd1-15 d1209-1273 R682A R685G K986P V987PPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
GWF135
Plasmid#133614PurposeANR-ANR-BFP-CAAXDepositorInsertANR-ANR-BFP-CAAX
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only
