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We narrowed to 5,797 results for: plasmid dna

Showing: 3151 - 3200 of 5797 results

  5. 10TALE_Pmin_fLuc

    Plasmid
    #160663
    Purpose
    Expression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domain
    Depositor
    Roman Jerala
    Article
    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20
    Insert
    10TALE_Pmin_fLuc
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    minimal
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160663-plasmid-map-sequence-id-345378

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  6. pTTP1A

    Plasmid
    #91721
    Purpose
    integration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNA
    Depositor
    Graham Hatfull
    Article
    Pham et al Microbiology. 2007 Aug;153(Pt 8):2711-23
    Type
    Empty backbone
    Use
    Integration-proficient plasmid vectors for mycoba…
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    May 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    91721-plasmid-map-sequence-id-178356

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  7. pTTP1B

    Plasmid
    #91723
    Purpose
    integration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNA
    Depositor
    Graham Hatfull
    Article
    Pham et al Microbiology. 2007 Aug;153(Pt 8):2711-23
    Type
    Empty backbone
    Use
    Integration-proficient plasmid vectors for mycoba…
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    May 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    91723-plasmid-map-sequence-id-178357

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  8. pET-MBP-Geo_st

    Plasmid
    #87700
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP
    Depositor
    Jennifer Doudna
    Insert
    GeoCas9
    Use
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87700-plasmid-map-sequence-id-174647

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  9. LgBiT-CALM1/SmBiT-ADCY1.1 BiBiT Vector

    Plasmid
    #237118
    Purpose
    Express LgBiT-CALM1/SmBiT-ADCY1.1 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    CALM1 (CALM1 Human)
    ADCY1 (ADCY1 Human)
    Use
    Tags
    LgBiT and SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237118-plasmid-map-sequence-id-474717

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  10. SmBiT-KRAS 4B G12C BiBiT Ready Vector

    Plasmid
    #237079
    Purpose
    Express SmBiT-KRAS 4B G12C Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Use
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    G12C
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237079-plasmid-map-sequence-id-473308

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  11. SmBiT-KRAS(G12V) BiBiT Ready Vector

    Plasmid
    #238832
    Purpose
    Express SmBiT-KRAS(G12V) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Use
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    G12V
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238832-plasmid-map-sequence-id-479923

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  12. NanoBRET Assay Vector, NanoLuc-KRas(G12C)

    Plasmid
    #238582
    Purpose
    Express NanoLuc-KRas(G12C) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Use
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    G12C
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238582-plasmid-map-sequence-id-478460

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  13. NanoBRET Assay Vector, NanoLuc-KRas(G12D)

    Plasmid
    #238581
    Purpose
    Express NanoLuc-KRas(G12D) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Use
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    G12D
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238581-plasmid-map-sequence-id-481209

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  14. NanoBRET Assay Vector, HaloTag-KRAS(G12V)

    Plasmid
    #238570
    Purpose
    Express HaloTag-KRAS(G12V) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Use
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    G12V
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238570-plasmid-map-sequence-id-485890

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  15. LgBiT-KRAS 4B(G12V) BiBiT-Ready Vector

    Plasmid
    #237076
    Purpose
    Express LgBiT-KRAS 4B(G12V) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Use
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    G12V
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237076-plasmid-map-sequence-id-471923

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  16. LgBiT-KRAS 4B G12C BiBiT Ready Vector

    Plasmid
    #237078
    Purpose
    Express LgBiT-KRAS 4B G12C Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Use
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    G12C
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237078-plasmid-map-sequence-id-471925

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  17. LgBiT-CYBA:CYBB-SmBiT vector

    Plasmid
    #237059
    Purpose
    Express LgBiT-CYBA:CYBB-SmBiT Fusion Proteins in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    CYBA (CYBA Human)
    CYBB (CYBB Human)
    Use
    Tags
    LgBiT and SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237059-plasmid-map-sequence-id-471912

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  18. NanoBRET Assay Vector, HaloTag-KRAS(G13D)

    Plasmid
    #236856
    Purpose
    Express HaloTag(R)-KRAS(G13D) in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Use
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    G13D
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236856-plasmid-map-sequence-id-471852

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  19. TBXTA-c021

    Plasmid
    #139758
    Purpose
    Protein expression/crystallography (PDB:5QT0)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Use
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-N211
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139758-plasmid-map-sequence-id-296541

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  20. TBXTA-c024

    Plasmid
    #139761
    Purpose
    Protein expression/GST pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Use
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-D225
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139761-plasmid-map-sequence-id-296544

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  21. TBXTA-c023

    Plasmid
    #139760
    Purpose
    Protein expression/GST pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Use
    Tags
    His6-GST-TEV
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41-D225
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139760-plasmid-map-sequence-id-296543

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  22. pGEM5Z(+)-EGFP

    Plasmid
    #65206
    Purpose
    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Restriction enzyme recognition sites added either…
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  24. pDONR207 SARS-CoV-2 NSP3

    Plasmid
    #141257
    Purpose
    Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Has Service
    Cloning Grade DNA
    Insert
    NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available Since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141257-plasmid-map-sequence-id-288044

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  26. pDONR207 SARS-CoV-2 E

    Plasmid
    #141273
    Purpose
    Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Has Service
    Cloning Grade DNA
    Insert
    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available Since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141273-plasmid-map-sequence-id-288100

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  27. pDONR207 SARS-CoV-2 M

    Plasmid
    #141274
    Purpose
    Gateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Has Service
    Cloning Grade DNA
    Insert
    M (M SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available Since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141274-plasmid-map-sequence-id-288102

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  29. pAAV-i53-DM

    Plasmid
    #92171
    Purpose
    recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant that has reverted mutations L69P and V70L (i53-DM)
    Depositor
    Daniel Durocher
    Article
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Insert
    i53-DM
    Use
    AAV
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A, P69L, and L70V mutations and no…
    Promoter
    CMV
    Available Since
    July 13, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    92171-plasmid-map-sequence-id-182616

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  30. pET-MBP-NLS-Geo_st

    Plasmid
    #87703
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS
    Depositor
    Jennifer Doudna
    Insert
    GeoCas9
    Use
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87703-plasmid-map-sequence-id-176992

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  31. pSL1142 (pSPIN, pBBR1 backbone)

    Plasmid
    #160730
    Purpose
    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available Since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    160730-plasmid-map-sequence-id-357931

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  32. lenti sgRNA(MS2)_puro backbone

    Plasmid
    #73795
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73795-plasmid-map-sequence-id-181202

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  33. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available Since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

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  37. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    160729-plasmid-map-sequence-id-322926

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  38. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid
    #160734
    Purpose
    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available Since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    160734-plasmid-map-sequence-id-318595

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  39. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73797-plasmid-map-sequence-id-196924

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  40. pSL1425 (pSPIN, pCDF backbone)

    Plasmid
    #160735
    Purpose
    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available Since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    160735-plasmid-map-sequence-id-317231

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  43. psiCHECK2-let-7 MT

    Plasmid
    #78261
    Purpose
    psiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene
    Depositor
    Joanne Weidhaas
    Article
    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10
    Insert
    mutated let-7 target site
    Use
    Luciferase; Microrna activity
    Tags
    Expression
    Mammalian
    Mutation
    nucleotides 2-5 and 10-11 changed from CTCC to GA…
    Promoter
    Available Since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    78261-plasmid-map-sequence-id-149478

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  47. miniABEmax (pRZ900)

    Plasmid
    #131311
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Insert
    bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131311-plasmid-map-sequence-id-254652

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Showing: 3151 - 3200 of 5797 results