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20XUAS IVS Jaws mVenus_tr

Plasmid

#111552

Purpose

Optogenetics in Drosophila: UAS-induced expression of Jaws mVenus

Depositor

Vivek Jayaraman

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mVenus_tr

Use

Tags

Expression

Insect

Mutation

wt

Promoter

hsp70

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS1932

Plasmid

#79781

Purpose

High copy number pUC vector containing Ple259 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple259

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Expression

Mutation

Promoter

Ple259 (PAX6 MiniPromoter)

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-Flag-Sintbad

Plasmid

#234668

Purpose

expresses SINTBAD (TBKBP1) in mammalian cells

Depositor

Lienhard Schmitz

Article

Saul et al iScience. 2019 Sep 27;19:527-544. doi: 1

Insert

SINTBAD (TBKBP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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T40A+Q54A

Plasmid

#217913

Purpose

A plasmid encoding the hydrogel-forming protein PXP with both the T40A and Q54A coil mutations

Depositor

Cole DeForest

Article

Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

Insert

PXP T40A+Q54A

Use

Tags

6x His (N and C term)

Expression

Bacterial

Mutation

Threonine at position 41 and 253 mutated to Alani…

Promoter

T5

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCas-mut4/1_2_VB

Plasmid

#186444

Purpose

Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Cas4 domain (K81A)

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut4x

Plasmid

#182256

Purpose

Human PDAP1 3` UTR region mutated in 4x binding site for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PDAP1 gene mutated in 4 binding sites for mir-150

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut2x

Plasmid

#182254

Purpose

Human PDAP1 3` UTR region mutated in 2x binding site for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PDAP1 gene mutated in 2 binding sites for mir-150

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut1x

Plasmid

#182253

Purpose

Human PDAP1 3` UTR region mutated in 1x binding site for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PDAP1 gene mutated in 1 binding site for mir-150

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut3x

Plasmid

#182255

Purpose

Human PDAP1 3` UTR region mutated in 3x binding site for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PDAP1 gene mutated in 3 binding sites for mir-150

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-anti-MIR328-3p

Plasmid

#153319

Purpose

Expresses zsGreen fused to a miR-328-3p sponge and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

zsGreen with MIR328-3p sponge

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-anti-MIR211-5p

Plasmid

#153318

Purpose

Expresses zsGreen fused to a miR-211-5p sponge and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

zsGreen with MIR211-5p sponge

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGA_pET28

Plasmid

#167326

Purpose

Expresses TFAM HMGA mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGA domain (43-122)

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGA_linker_pET28

Plasmid

#167327

Purpose

Expresses TFAM HMGA+linker mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGA and linker doma…

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_HMGB_ctail_pET28

Plasmid

#167330

Purpose

Expresses TFAM HMGB+C-tatil mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGB and C-tail doma…

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_dC_pET28

Plasmid

#167328

Purpose

Expresses TFAM dC-tail mutant in bacterial cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Truncation mutant containing HMGA, linker and HMG…

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgALCAM

Plasmid

#83929

Purpose

Lentiviral vector expressing an sgRNA targeting ALCAM. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgALCAM

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgSLC35B2

Plasmid

#83932

Purpose

Lentiviral vector expressing an sgRNA targeting SLC35B2 NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgSLC35B2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgTPST2

Plasmid

#83933

Purpose

Lentiviral vector expressing an sgRNA targeting TPST2 NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgTPST2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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EC71102

Plasmid

#154065

Purpose

Level 0 Golden Gate vector; Unmodifed CRE

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

CRE

Use

Synthetic Biology; Standard cloning vector used b…

Tags

Expression

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS2046

Plasmid

#79654

Purpose

ssAAV genome with Ple257 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple257-EmGFP WPRE

Use

AAV

Tags

Expression

Mutation

Promoter

PAX6 Enhancer

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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