We narrowed to 24,118 results for: CRISPR

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pKEW106-MbCas12a-58069

Plasmid

#115653

Purpose

Expresses MbCas12a in bacteria from M. bovoculi strain 58069, under TetR control

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

MbCas12a

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0020

Plasmid

#117531

Purpose

Level0 Golden Gate part: CDS, SaCas9

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (with stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMB67

Plasmid

#47947

Purpose

Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and egl-13 NLS

Expression

Worm

Promoter

hsp-16.48

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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peft-3::cas-9::tbb-2 3'UTR

Plasmid

#48960

Purpose

this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.

Depositor

Mario de Bono

Article

Chen et al Nucleic Acids Res. 2013 Sep 5.

Insert

cas9

Use

CRISPR

Promoter

eef-1A.1 (eft-3)

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCR1003

Plasmid

#71821

Purpose

Express Streptococcus pyogenes dCas9 (D10A, H840A)

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Insert

T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)

Use

CRISPR

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_GABPA_2

Plasmid

#64255

Purpose

Expresses gRNA against human GABPA along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pKEW107-MbCas12a-33362

Plasmid

#115654

Purpose

Expresses MbCas12a in bacteria from M. bovoculi strain 33362, under TetR control

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

MbCas12a

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available Since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0013

Plasmid

#117521

Purpose

Level0 Golden Gate part: CDS, SpCas9-p

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-p (with stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_EBFP2

Plasmid

#167857

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCR1055

Plasmid

#71823

Purpose

Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Insert

T7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS

Use

CRISPR

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_GABPA_1

Plasmid

#64254

Purpose

Expresses gRNA against human GABPA along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJA50

Plasmid

#59931

Purpose

gRNA for cleavage at unc-58(e665) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

unc-58(e665) gRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pJA54

Plasmid

#60212

Purpose

cleavage near e1350 locus in sqt-1

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

sqt-1(e1350) gRNA1

Use

CRISPR

Promoter

U6

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-GBP-dCas9-mRFP

Plasmid

#102933

Purpose

Expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)

Depositor

Sebastian Bultmann

Article

Anton et al Nucleus. 2017 May 4;8(3):279-286. doi: 1

Insert

dCas9

Use

CRISPR

Tags

GBP (GFP binding protein) and mRFP

Expression

Mammalian

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only
pSCBE3-HF

Plasmid

#133967

Purpose

Expresses HF-BE3 CRISPR base editor and gRNA scaffold in Streptomyces

Depositor

Yuhui Sun

Article

Zhong et al bioRxiv 630137

Insert

HF-BE3

Use

CRISPR and Synthetic Biology

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_ATF1_1

Plasmid

#64690

Purpose

Expresses gRNA against human ATF1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJA55

Plasmid

#59935

Purpose

gRNA for cleavage near sqt-1(e1350) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

sqt-1(e1350) gRNA2

Use

CRISPR

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pBLlSh2

Plasmid

#170981

Purpose

Expression of ShCAST under control of lacI Plac promoter/repressor pair.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Inserts

lacI
ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pLlac01

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAct-dCBEevoAPOBEC1

Plasmid

#195514

Purpose

Ubiquitous expression of dCBEevoAPOBEC1 in Drosophila

Depositor

Michael Boutros

Article

Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

Insert

dCBE-evoAPOBEC1

Use

CRISPR

Expression

Insect

Promoter

act5C

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJA59

Plasmid

#59932

Purpose

gRNA for cleavage at unc-109(n499) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

unc-109(n499) gRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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JME4393

Plasmid

#129657

Purpose

LYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Article

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-scramble

Plasmid

#62285

Purpose

expression of scramble sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

scramble sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLdSaCN

Plasmid

#123261

Purpose

Expresses Staphylococcus aureus Cas9 (SaCas9) and its gRNA in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Insert

gRNA and SaCas9

Use

CRISPR; Leishmania

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCF823_EFS-Cas9-PuroR_lenti

Plasmid

#138317

Purpose

Lenti expression of SpyCas9 with Puro selection

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

SpyCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSL1166

Plasmid

#129520

Purpose

pDEF-SpCas9::GFP, For deletion of pyalba4, PyBiP-full promoter, 2nd Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

SpCas9

Use

Plasmodium, rodent-infectious species

Tags

3XFLAG-NLS

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBBK31 pHomL-TDH3p-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179015

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-1

Plasmid

#164865

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-1

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A2T

Plasmid

#62258

Purpose

expression of A2T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A2T sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPOR0SP0007

Plasmid

#117534

Purpose

Level0 Golden Gate part: CDS, LbCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

LbCas12a (with stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A1T

Plasmid

#62256

Purpose

expression of A1T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A1T sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge