We narrowed to 7,456 results for: Lif
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Plasmid#99089PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea Runt-1 (also known as runt)DepositorInsertRunt-1 (also known as runt) in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3.2mar(T->G)-Luc
Plasmid#63697Purpose3.2 kb marine armor plate enhancer with a single T->G bp change driving Luciferase expressionDepositorInsert3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change
UseTagsLuciferaseExpressionMammalianMutationPromoterTATA boxAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3.2mar(T->G)-GFP
Plasmid#63695Purpose3.2 kb marine armor plate enhancer with a single T->G bp change driving GFP reporterDepositorInsert3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change
UseTagsEGFPExpressionMammalianMutationT->G bp changePromoterhsp70Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (5'GAC)
Plasmid#170127PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(5'GAC)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (3'GAC)
Plasmid#170129PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(3'GAC)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn NS34a-msGFP-'S-mito
Plasmid#158768PurposeLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseLentiviralTagsExpressionMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-SYNJ2BP-1
Plasmid#92159PurposeCRISPR guide RNA targeting human SYNJ2BPDepositorInsertSYNJ2BP sgRNA-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-SYNJ2BP-2
Plasmid#92160PurposeCRISPR guide RNA targeting human SYNJ2BPDepositorInsertSYNJ2BP sgRNA-2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
UAS-u(L)Cas9
Plasmid#127385PurposeExpresses Cas9 at low levelDepositorInsertuORF(L)-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-deltaTMD SYT7alpha
Plasmid#179718PurposeLentiviral expression of synaptic vesicle-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTag
Plasmid#179719PurposeLentiviral expression of synaptic vesicle-targeted Halo tagged mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn PM-SYT7alpha deltaTMD-HaloTag
Plasmid#179721PurposeLentiviral expression of plasma membrane-targeted Halo tagged mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD
Plasmid#179722PurposeLentiviral expression of lysosome-targeted mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag
Plasmid#179723PurposeLentiviral expression of lysosome-targeted Halo tagged mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) U6-SYT7 sgRNA 777 HaloTag
Plasmid#179724PurposeU6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITIDepositorInsertsgRNA and HaloTag
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) CamKII sf iGluSnFR S72A
Plasmid#179726PurposeLentiviral expression of iGluSnFR biosensor for monitoring synaptic vesicle exocytosis and glutamate releaseDepositorInsertiGluSnFR
UseLentiviralTagsExpressionMutationS72APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pHR CD19 tandem CAR
Plasmid#113019Purposefor lentivirus production of CD19 tandem CARDepositorInsertCD19 CAR-P tandem FcR gamma intracellular and PI3k recruiting domain from CD19 (CD19 Human, Synthetic, Mouse)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:CoChR-tdTomato
Plasmid#124233PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomatoDepositorInsertCoChR-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:ChR2(H134R)-tdTomato
Plasmid#124237PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomatoDepositorInsertChR2(H134R)-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_mScarlet-LAMP1
Plasmid#185138PurposeExpresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetimeDepositorInsertLysosome-associated membrane glycoprotein 1 (Lamp1 Rat, Synthetic)
UseTagsmScarletExpressionMammalianMutationdeleted amino acids 1-21 of LAMP1PromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 V5 mDispatched WT 2x HA
Plasmid#126410PurposeExpression of V5 Dispatched WT HA in mammalian cell lines.DepositorInsertm-Dispatched1 (Disp1 Mouse)
UseTagsHAExpressionMammalianMutationV5 iserted between A106 and A107PromoterAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
N1-Tq-Ca-FLITS
Plasmid#191454PurposeGenetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changesDepositorInsertTq-Ca-FLITS
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ heiCas9
Plasmid#186929PurposeFor in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLSDepositorInsertmyc-flexible linker-oNLS-Cas9-oNLS
UseCRISPRTagsmyc-flexible linker-optimizedNLS and optimizedNLSExpressionMammalianMutationPromotersp6Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti_dCas9-KRAB-MeCP2
Plasmid#122205PurposeBipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.DepositorInsertdCas9-KRAB-MeCP2
UseLentiviralTagsKRAB-MeCP2ExpressionMammalianMutationCas9 (D10A, H840A)PromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DsRed_IRES_EGFP
Plasmid#92194PurposeLentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screenDepositorInsertDsRed-IRES-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
RBD-Fc
Plasmid#164221PurposeExpresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporterDepositorInsertSpike RBD (S SARS-CoV)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Puro-CAG-mNG2(1-10)
Plasmid#206042PurposeRepair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.DepositorInsertAAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
UseCRISPR and Synthetic Biology; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mCherry-CHMP2A
Plasmid#115330Purposeexpresses mCherry-CHMP2A in mammalian cellsDepositorInsertCHMP2A (CHMP2A Human)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-VAPB
Plasmid#108126PurposeER markerDepositorInsertmCherry:VAPB (VAPB Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiSAM v2 (Puro)
Plasmid#92062PurposeModified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 (sgRNA) and EF1a (dCas9-VP64)Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only