Addgene

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Cx55.5:rasGFP

Plasmid

#105939

Purpose

in vitro transcription

Depositor

Caren Norden

Article

Weber et al Cell Rep. 2014 Apr 24;7(2):386-97. doi:

Insert

Ras

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO-shCIAP2-B

Plasmid

#44132

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

CIAP2

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
pEnt L1L3 EF1a-tTA-3

Plasmid

#24415

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2010

Availability

Academic Institutions and Nonprofits only

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px459 EQR

Plasmid

#101732

Purpose

Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 EQR

Use

Tags

Expression

Mammalian

Mutation

VQR

Promoter

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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FmnI IS04 cDNA/pcDNA3

Plasmid

#19321

Depositor

Philip Leder

Article

Dettenhofer et al PLoS ONE. 2008 . 3(6):e2497.

Insert

FmnI Iso4 cDNA (Fmn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ypet-RBD

Plasmid

#105291

Purpose

To measure Rho activity by binding and FRET analysis

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

RBD (Rtkn Mouse)

Use

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 7-89

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pN1-NLS-Q2m

Plasmid

#129319

Purpose

QUEEN ATP sensor for 25degC, nucleus

Depositor

Yasushi Okada

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-2m

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pZH509

Plasmid

#102664

Purpose

Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Syx2

Plasmid

#35705

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx2 (Plekhg5 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pSBbi-TIR1-tTA/Neo

Plasmid

#171684

Purpose

constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

Neomycin

Use

Tags

Expression

Mammalian

Mutation

Promoter

RPBSA promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mRFP-Syx1

Plasmid

#35707

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx1 (Plekhg5 Mouse)

Use

Tags

mRFP

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pTracer-EF-mFxh

Plasmid

#21927

Depositor

Mariano Garcia-Blanco

Article

Baraniak et al Mol Cell Biol. 2006 Feb . 26(4):1209-22.

Insert

Fxh (Rbfox2 Mouse)

Use

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2009

Availability

Academic Institutions and Nonprofits only
pMSCV Cyclin D2-short

Plasmid

#21655

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 (CCND2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV Cyclin D2 long-wt

Plasmid

#21646

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 (CCND2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pHR Src deltaSH2/3-TagBFP

Plasmid

#171033

Purpose

TagBFP-tagged Src variant lacking SH2 and SH3 domains.

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Src

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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B108-GFP-IRES-AP

Plasmid

#73988

Purpose

B108 enhancer of Blimp1 gene drives EGFP expression.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

B108 enhancer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pL_FR_Hygro

Plasmid

#127171

Purpose

Frameshift reporter (control plasmid for screening)

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

Frameshift Reporter

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_P3-ehaA_LacI

Plasmid

#186315

Purpose

Plasmid for inducible E coli expression of the P3 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

P3-ehaA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_AP4-ehaA_LacI

Plasmid

#186316

Purpose

Plasmid for inducible E coli expression of the AP4 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

AP4-ehaA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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M16 pCS-twhh-bGFP

Plasmid

#17153

Depositor

Randall Moon

Article

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

twhh promoter (shhb Zebrafish)

Use

Tags

bGFP

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pNB330

Plasmid

#60147

Purpose

Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Use

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Mutation

Promoter

MET17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pTCW008

Plasmid

#83526

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoter

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1J2-mfalpha2

Use

Tags

Expression

Yeast

Mutation

Promoter

pFUS1J2

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IL2sp-6His-Asprosin plasmid

Plasmid

#190683

Purpose

Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

Depositor

Atul Chopra

Article

Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

Insert

IL2-6xHis-Asprosin

Use

Tags

IL2 signal peptide, 6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Rab18b cds in pGem-T Easy

Plasmid

#80539

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab18b (rab18b Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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Rab18a cds in pGem-T Easy

Plasmid

#80538

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab18a (rab18a Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEnt L1L3 tTA-2

Plasmid

#24414

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV syn CCL20-pre-mGRASP

Plasmid

#173117

Purpose

Expresses CCL20 sender construct in neurons

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCL20-pre-mGRASP

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV syn Arrestin-TEVp-V5

Plasmid

#173118

Purpose

Expresses arrestin receiver construct in neurons

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-TEVp-V5

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pZH512

Plasmid

#102666

Purpose

Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-mPlexinD1

Plasmid

#16424

Depositor

Jonathan Epstein

Article

Gitler et al Dev Cell. 2004 Jul . 7(1):107-16.

Insert

Plexin D1 (Plxnd1 Mouse)

Use

In situ template

Tags

Expression

Mutation

None

Promoter

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pEnt R3L2-MCS

Plasmid

#26799

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

multiple cloning sequence

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pRoTnsABCDQ

Plasmid

#178772

Purpose

Expression of RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalis

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalis

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pN1-NLS-Q7mu

Plasmid

#129314

Purpose

Positive control for ATP measurement, nucleus

Depositor

Yasushi Okada

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-7mu

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN3

Plasmid

#79257

Purpose

Expresses EGFP-tagged DmPAN3 in S2 cells

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

PAN3 (CG11486 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Expression

Mutation

Promoter

Ac5 promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pME-FusionRed-NS

Plasmid

#80500

Purpose

Multisite gateway entry clone for adding N-terminal fusions of FusionRed

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

FusionRed (no STOP)

Use

Multisite gateway middle entry clone

Tags

Expression

Mutation

Promoter

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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CRISPRmTmG2

Plasmid

#69992

Purpose

The CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

gRNA that targets near LoxP sites

Use

Tags

Expression

Mutation

Promoter

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pZH511

Plasmid

#102665

Purpose

Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLU-YFP-ICP0FxE

Plasmid

#134564

Purpose

Expresses YFP-ICP0 fusion protein with a RING finger deletion (FXE)

Depositor

Susan Janicki

Article

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Insert

YFP-ICP0

Use

Tags

None

Expression

Mammalian

Mutation

RING finger deletion (FXE)

Promoter

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pOZ-Pax7-FH-C-puro

Plasmid

#32518

Depositor

Andrew Lassar

Article

Kumar et al Mol Biol Cell. 2009 Jul;20(14):3170-7. E

Insert

Pax7d (Pax7 Mouse)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
Sox17 Flox

Plasmid

#23335

Depositor

Sean Morrison

Article

Kim et al Cell. 2007 Aug 10. 130(3):470-83.

Insert

Sex determining region Y-box containing gene 17 (Sox17 Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

None

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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