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Plasmid#163365PurposeExpress HA- and Clover-tagged HIF-1alpha wildtype plasmidDepositorInsertHypoxia Inducible Factor 1 alpha (HIF1A Human)
UseTagsHA- and Clover-taggedExpressionMammalianMutationWildtypePromoterCMV PromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pESC-MET
Plasmid#222482PurposeCo-expression of two genes in yeast with FLAG and MYC tags and MET15 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-LYS
Plasmid#222483PurposeCo-expression of two genes in yeast with FLAG and MYC tags and LYS2 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-TYR
Plasmid#222484PurposeCo-expression of two genes in yeast with FLAG and MYC tags and TYR1 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-THR
Plasmid#222479PurposeCo-expression of two genes in yeast with FLAG and MYC tags and THR1 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-ADE
Plasmid#222480PurposeCo-expression of two genes in yeast with FLAG and MYC tags and ADE2 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-ARG
Plasmid#222481PurposeCo-expression of two genes in yeast with FLAG and MYC tags and ARG1 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10/RSV_XS3B-miniXon_Luciferase
Plasmid#217302PurposePlasmid containing the SF3B3-XS3B/Firefly luciferase cassette, a modification of the SF3B3-miniXon system. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-XS3B by LDepositorInsertXS3B-miniXon
UseLuciferaseTagsExpressionMammalianMutationKozak ATG initiation sequence in middle exonPromoterRSVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pE_Cre_lambdaCos
Plasmid#173785PurposeThe pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.DepositorInsertCre
UseCre/Lox and Synthetic BiologyTagsExpressionMutationS102Y and S279Y- please see depositor commentsPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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ACE2 g1 + Cas9
Plasmid#153011PurposeA guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2DepositorInsertCas9 + ACE2 gRNA (ACE2 Human)
UseCRISPR and LentiviralTagsTagBFP2ExpressionMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a
Plasmid#159908PurposeMutagenesis of Htr2aDepositorInsertHtr2a (Htr2a Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST co hPHGDH
Plasmid#154915PurposeExpress codon optimized human PHGDHDepositorInsertPHGDH, codon optimized (PHGDH Synthetic, Human)
UseRetroviralTagsExpressionMammalianMutationCodon optimized for expression in human cellsPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
ACE2 g4 + Cas9
Plasmid#153014PurposeA guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2DepositorInsertCas9 + ACE2 gRNA (ACE2 Human)
UseLentiviralTagsTagBFP2ExpressionMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCJ155
Plasmid#175054PurposepsbEFLJ integrative plasmid expresses CRE recombinase from the rbcL promoter uses zeocin to integrateDepositorInsertCRE
UseUnspecifiedTagsExpressionMutationPromoterrbcLXSAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro SARS-CoV2 N P2A eGFP-NLS
Plasmid#154399PurposePiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFPDepositorInsertSARS-CoV2 N Protein (N SARS-CoV2)
UsePiggybacTagsmCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
punc-119cR
Plasmid#21596DepositorInsertunc-119:mCherry (unc-119 Nematode)
UseAdds unc-119 marker to amp resistant plasmidsTagsmCherryExpressionWormMutationN/APromoterAvailable sinceAug. 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
CHD1 (CHD1A-c107)
Plasmid#110253PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCHD1 (CHD1 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7 - lacOAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
ABCB8 (ABCB8A-c016)
Plasmid#110266PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertABCB8 (ABCB8 Human)
UseTagsTEV-His10-FlagExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only