57,943 results

1. pH2B-mNG2(11)

   Plasmid

   #206043

   Purpose

   Plasmid expressing a H2B-mNG2(11) fusion in mammalian cells for complementation with the mNG2(1-10) fragment.

   Depositor

   Alisa Piekny

   Article

   Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

   Insert

   H2B gene fused to the mNG2(11) fragment
   

   Use

   Synthetic Biology

   Tags

   H2B-mNG2(11)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-CMV mCherry-GFP-LC3B WT

   Plasmid

   #123230

   Purpose

   Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST53-LRRK2-R1441C

   Plasmid

   #25046

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Arginine 1441 mutated to Cysteine

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pLKO.1-TetON-Puro-HIF1a 0819

   Plasmid

   #118704

   Purpose

   Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   shRNA against human HIF1a
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGFP-ATIC

   Plasmid

   #99110

   Purpose

   Expresses human GFP-ATIC fusion in mammalian cells

   Depositor

   Stephen Benkovic

   Article

   An et al Science. 2008 Apr 4;320(5872):103-6. doi

   Insert

   ATIC (ATIC Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S65T mutation in GFP gene

   Promoter

   CMV

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMDR1-1202

   Plasmid

   #37627

   Depositor

   Kathleen Scotto

   Article

   Hu et al J Biol Chem. 2000 Jan 28;275(4):2979-85.

   Insert

   MDR1 promoter (ABCB1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

   Plasmid

   #167296

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   BAX (BAX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe-Kras G12D

   Plasmid

   #58902

   Purpose

   Expresses human Kras with a G12D Mutation

   Depositor

   Channing Der

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G12D mutation

   Promoter

   Available since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1-TetON-Puro-HIF1a 3809

   Plasmid

   #118705

   Purpose

   Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   shRNA against human HIF1a
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-HP1alpha

    Plasmid

    #17652

    Depositor

    Tom Misteli

    Article

    Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

    Insert

    HP1 alpha (CBX5 Human)
    

    Use

    Tags

    GFP and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lyn-FKBP-FKBP-CFP (LF2C)

    Plasmid

    #20149

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    Lyn-FKBP-FKBP-CFP (FKBP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. ppyCAG_RNaseH1_WT

    Plasmid

    #111906

    Purpose

    Express RNASEH1 in mammalian cell.

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+) EGFP-Rac1(WT)

    Plasmid

    #224278

    Purpose

    Cell transfection and expression of Rac1

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV V5-hRnf185

    Plasmid

    #175127

    Purpose

    Lentiviral expression of V5-tagged human RNF185

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    RNF185 (RNF185 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRUPATH Triple GaoA

    Plasmid

    #196051

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. SAFB1-EGFP

    Plasmid

    #32746

    Depositor

    Steffi Oesterreich

    Article

    Townson et al Clin Cancer Res. 2000 Sep;6(9):3788-96.

    Insert

    SAFB1 (SAFB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pProEx RalA(G23C) CL

    Plasmid

    #224263

    Purpose

    Protein purification of RalA (G23C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    RalA (RALA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G23C

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO-Tet-On-ARNTL-shRNA1

    Plasmid

    #166913

    Purpose

    Lentivirus for inducible knockdown of human ARNTL(BMAL1)

    Depositor

    Roland Friedel

    Insert

    ARNTL shRNA (BMAL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Str-KDEL_flGalT-SBP-EGFP

    Plasmid

    #65272

    Purpose

    synchronize trafficking of GalT from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    galactosidase
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT3-EF1a-c-Met

    Plasmid

    #31784

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    Met (MET Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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