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Showing: 1981 - 2010 of 5871 results

  4. pcDNA3.1-p15 Bid-KKAA-GFP

    Plasmid
    #191970
    Purpose
    Expression of the active p15 cBid KKAA mutant, chimera with GFP.
    Depositor
    Luca Scorrano
    Insert
    p15 - cBid KKAA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    KKAA: Lys 157 and Lys 158 into Ala
    Promoter
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    191970-plasmid-map-sequence-id-379644

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  5. pcDNA3.1(+)-DnaJB1(H33Q)-PKAcat-mCherry

    Plasmid
    #181854
    Purpose
    mCherry-tagged DnaJB1-PKA catalytic subunit fusion with H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Histidine 33 in DnaJB1 changed to glutamine.
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181854-plasmid-map-sequence-id-362794

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  10. pcDNA3.1-kappa-myc-dK-2xG4S-TMst

    Plasmid
    #145766
    Purpose
    Expresses myc-dL5(E52K, L91S) low affinity Fluorogen Activating Protein (FAP) on the extracellular surface anchored to the mammalian cell plasma membrane with the PDGFR transmembrane domain.
    Depositor
    Marcel Bruchez
    Article
    Carpenter et al ACS Chem Biol. 2020 Aug 17. doi: 10.1021
    Insert
    kappa-myc-dK-2xG4S-TMst
    Use
    Tags
    Expression
    Mammalian
    Mutation
    The dK FAP is dL5 with mutations at E50K, L89S, a…
    Promoter
    CMV
    Available since
    Jan. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    145766-plasmid-map-sequence-id-353271

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  20. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

    Plasmid
    #131256
    Purpose
    Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Contains point mutations that convert USP7 aspart…
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131256-plasmid-map-sequence-id-267711

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Showing: 1981 - 2010 of 5871 results