We narrowed to 5,818 results for: pcas

1. pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

   Plasmid

   #107729

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBW829_pCAG-pExpr-LUT

   Plasmid

   #87554

   Purpose

   Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   EGFP
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BII-gR-PtW-eSpCas9

   Plasmid

   #133357

   Purpose

   Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Tags

   HA-tagged eSpCas9

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-2XHA-Borcs7

   Plasmid

   #118747

   Purpose

   Expresses 2XHA tagged Borcs7

   Depositor

   Beverly Koller

   Insert

   Borcs7 cDNA (2010012O05Rik Mouse)
   

   Tags

   2XHA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-Scarlet-Borcs7

   Plasmid

   #118750

   Purpose

   Expresses scarlet tagged Borcs7-Q87X

   Depositor

   Beverly Koller

   Insert

   Borcs7-Q87X cDNA (2010012O05Rik Mouse)
   

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Q87X

   Promoter

   CAG

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-TetR-Rad18UBD

   Plasmid

   #119010

   Purpose

   Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   TetR
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-BsCas12a-2AeGFP

   Plasmid

   #123633

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   BsCas12a
   

   Tags

   3xHA tag

   Expression

   Mammalian

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCaSpeR eGFP-dhd

   Plasmid

   #113004

   Purpose

   P element vector for transposon insertion into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Insert

   dhd (dhd Fly)
   

   Use

   P insertion vector

   Tags

   eGFP

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCaSpeR dhd-eGFP

   Plasmid

   #113003

   Purpose

   P element vector for transposon insertion of dhd-eGFP into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Insert

   dhd (dhd Fly)
   

   Use

   P insertion vector

   Tags

   eGFP

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-Trex2-bpA

    Plasmid

    #86984

    Purpose

    Expression of mouse Trex2

    Depositor

    Ralf Kuehn

    Article

    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

    Insert

    mouse Trex2 coding region (Trex2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCas9–mKate2ps–EgRNA

    Plasmid

    #62716

    Purpose

    Empty; no U6 gRNA cassette

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Expression

    Mammalian

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCAG-PrCas12a-2AeGFP

    Plasmid

    #123636

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    PrCas12a
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSpCAS9n(BB)-2A-puro DNM1, A

    Plasmid

    #107795

    Purpose

    gRNA vector for knock-in gene editing at human DNM1 locus

    Depositor

    Sandra Schmid

    Article

    Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

    Insert

    gRNA targeting human DNM1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpCAS9n(BB)-2A-puro DNM1, B

    Plasmid

    #107796

    Purpose

    gRNA vector for knock-in gene editing at human DNM1 locus

    Depositor

    Sandra Schmid

    Article

    Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

    Insert

    gRNA targeting human DNM1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX330_rActb sgRNA / hSpCas9

    Plasmid

    #172833

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Myc-gamma3 (in pCAGGS)

    Plasmid

    #118042

    Purpose

    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG3 (Gabrg3 Rat)
    

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX330_Tuba1b sgRNA / hSpCas9

    Plasmid

    #172835

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-BsCas12b-2AeGFP

    Plasmid

    #121948

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    BsCas12b
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS-hCD4-GCaMP2

    Plasmid

    #18932

    Depositor

    Karel Svoboda

    Article

    Mao et al PLoS ONE. 2008 . 3(3):e1796.

    Insert

    hCD4-GCaMP2 (CD4 Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pCAG-HDAC4 C-terminus

    Plasmid

    #71400

    Purpose

    CAG promoter, mammalian expression

    Depositor

    Bo Chen

    Article

    Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

    Insert

    HDAC4 C-terminus (HDAC4 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only