We narrowed to 5,538 results for: pcas

1. pCA24N-ligase-pprA

   Plasmid

   #87744

   Purpose

   IPTG-inducible expression of ligase-pprA fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Use

   Tags

   6xHis and codon optimized pprA (from Deinococcus …

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCaSpeR-act5cB-LexAQFw

   Plasmid

   #61311

   Purpose

   Expresses chimeric transactivator LexA_DBD:QF2w_AD in Drosophila

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   LexA_DBD::QF2w_AD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Changed EK on C-terminus of QF2 to KKKK

   Promoter

   Available since

   Jan. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCALNL-BsaI-EGFP

   Plasmid

   #123136

   Purpose

   Golden Gate acceptor vector for the assembly of EGFP-based mammalian recombinase reporters.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   EGFP, mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cBA

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG aconitase GFP

   Plasmid

   #66104

   Purpose

   expression of chicken aconitase GFP

   Depositor

   Martin Scaal, Felicitas Proels

   Article

   et al Development. 2015 Feb 15;142(4):665-71.

   Insert

   aconitase
   

   Use

   Chicken expression

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET-FLAG-eSpCas9

   Plasmid

   #126769

   Purpose

   Expression of increased fidelity eSpCas9 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCaSpeR3/ncd-gfp #10

   Plasmid

   #66082

   Purpose

   stable germline transformation of Drosophila to produce Ncd-GFP expression

   Depositor

   Sharyn Endow

   Article

   Endow et al J Cell Biol. 1997 Jun 16;137(6):1321-36.

   Insert

   Ncd kinesin-14 motor protein (ncd Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   D699L

   Promoter

   ncd promoter (3.1kb upstream region of ncd start …

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCAGGS-TAL-NC2

   Plasmid

   #43856

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

   Insert

   TALEN-FokI nuclease backbone
   

   Use

   Tags

   Flag and FokI nuclease

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-HDAC4 1-129

   Plasmid

   #71401

   Purpose

   CAG promoter, mammalian expression

   Depositor

   Bo Chen

   Article

   Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

   Insert

   HDAC4 1-129 (HDAC4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pBT255_(pCA-GFP4m)

   Plasmid

   #36877

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F64L; S65T; V163A; S175G

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer)

   Available since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCALNL-eGFP-Esp3I

    Plasmid

    #81226

    Purpose

    Cloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sites

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    Esp31-Neo-Esp31 deletion cassette upstream of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCBa

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-HDAC4-IGd2

    Plasmid

    #22536

    Purpose

    To be used for the RNAi screening purposes (not for HDAC4 expression); see paper

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    Histone Deacetylase 4 (HDAC4 Human)
    

    Use

    Tags

    IGd2 = Ires-destabilized GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pCAG-p21_T145A/S146A

    Plasmid

    #42623

    Depositor

    Philipp Mergenthaler

    Article

    Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

    Insert

    p21 (CDKN1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T145A/S146A

    Promoter

    CAG

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. CpcBfull-NaGLS fusion_CpcA

    Plasmid

    #74004

    Purpose

    Expresses the Nicotiana attenuata GLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

    Depositor

    Anastasios Melis

    Article

    Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

    Insert

    NaGLS and CmR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cpc

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-△MTS-TFAM-V5

    Plasmid

    #200934

    Purpose

    expresses △MTS-TFAM-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    Transcription Factor A, Mitochondrial (TFAM Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    deleted mitochondrial targeting sequence (MTS)

    Promoter

    CAG

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-MTS-TFAM-V5

    Plasmid

    #200813

    Purpose

    expresses MTS-TFAM in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    Transcription Factor A, Mitochondrial (TFAM Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-FLAG-BubR1

    Plasmid

    #200814

    Purpose

    expresses BubR1 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-MTS-EGFP-V5

    Plasmid

    #200816

    Purpose

    expresses MTS-EGFP-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    EGFP
    

    Use

    Tags

    V5 and mitochondrial targeting sequence (MTS)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCARGO-RMCE-Xist

    Plasmid

    #233268

    Purpose

    CARGO plasmid containing ~22kb dox-inducible Xist transgene

    Depositor

    Mauro Calabrese

    Article

    Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

    Insert

    22kb Xist gene, including introns
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCARGO-RMCE-Airn

    Plasmid

    #233266

    Purpose

    CARGO plasmid containing ~90kb dox-inducible Airn transgene

    Depositor

    Mauro Calabrese

    Article

    Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

    Insert

    Airn (Airn Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG_GST-TEV-WIPI1

    Plasmid

    #223798

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens, James Hurley

    Article

    Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

    Insert

    WIPI1 (WIPI1 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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