Addgene

We narrowed to 3,852 results for: pes

Showing: 2011 - 2040 of 3852 results

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Nrxn3α-AS4+

Plasmid

#184333

Purpose

Expression of mouse Nrx3-alpha containing insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

Depositor

Peter Scheiffele

Article

Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife

Insert

Nrxn3-alpha (Nrxn3 Mouse)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FDIO-COX4-dAPEX2

Plasmid

#117178

Purpose

Plasmid name in publication: pAAV-FDIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

COX4-dAPEX2

Use

AAV; Flp/frt

Tags

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hNR2F2-RFP

Plasmid

#22926

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

human nuclear receptor subfamily 2 group F member 2 promoter driving DsRedExpress (NR2F2 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pWPT-mCherry

Plasmid

#190605

Purpose

Obtained by cloning 5 stop codons in place of the EGFP Kozak sequence in pWPT-/mEGFP-1T-IRES-mCherry

Depositor

Alessandro Quattrone

Article

Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

Inserts

mEGFP
mCherry

Use

Lentiviral

Tags

Expression

Mutation

EGFP Kozak sequence has been replaced with a stre…

Promoter

EIF1-short

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HCMVgL-FLAG (TB40/E)

Plasmid

#136449

Purpose

Mammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tag

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein L (UL115 Human betaherpesvirus 5)

Use

Tags

Short SGGSG linker and FLAG tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EnhancedSynapsin-DIO-TVA950-EYFP-WPRE

Plasmid

#120269

Purpose

Allows for initial cellular transduction of EnvA-pseudotyped Rb via Cre-dependent expression of an avian TVA receptor fused with yellow fluorescent protein

Depositor

Edward Callaway

Article

Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

Insert

eSyn-DIO-TVA950:YFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10

Plasmid

#181859

Purpose

Part of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

cp-PKN-mRuby2-GFP(1-0)

Use

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FDIO-LACTB-dAPEX2

Plasmid

#117181

Purpose

Plasmid name in publication: pAAV-FDIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

LACTB-dAPEX2

Use

AAV; Flp/frt

Tags

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HCMVgL-FLAG (Merlin)

Plasmid

#136450

Purpose

Mammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tag

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein L (UL115 Human betaherpesvirus 5)

Use

Tags

Short SGGSG linker and FLAG tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pTXB1-TP4

Plasmid

#160087

Purpose

Bacterial expression of Tn5-APEX2 fusion protein with linker (GGGGS)4

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase/APEX2 peroxidase fusion protein

Use

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS2-Spike-D614G

Plasmid

#166850

Purpose

For expression of SARS-CoV-2 Spike D614G in mammalian cells (C9 tagged at C-term)

Depositor

Neville Sanjana

Article

Daniloski et al Elife. 2021 Feb 11;10. pii: 65365. doi:

Insert

SARS-CoV-2 Spike (S SARS-CoV-2 virus)

Use

Tags

C9

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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TS-PE2-N

Plasmid

#176649

Purpose

Expresses N-terminus of PE2 in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Inserts

N-terminus of PE2
Artificial SD

Use

AAV

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV and None

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE

Plasmid

#120268

Purpose

Permits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporter

Depositor

Edward Callaway

Article

Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

Insert

EF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-GLI(-)TAD

Plasmid

#37113

Depositor

David Walterhouse

Article

Yoon et al J Biol Chem. 1998 Feb 6;273(6):3496-501.

Insert

GLI1 (GLI1 Human)

Use

Tags

Expression

Mammalian

Mutation

deleted 1,409 bp AccI fragment from 3' end o…

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
pdCas12a(RuvC)

Plasmid

#186447

Purpose

Encodes catalytically inactive Cas12a (D917A, E1006A) from F. novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

dCas12a

Use

CRISPR

Tags

Expression

Mutation

D917A, E1006A

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr-D435A/E437A

Plasmid

#40829

Purpose

Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

Depositor

Phillip Zamore

Article

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

Nbr D435A/E437A (Nbr Fly)

Use

Tags

3xFLAG and 6xMyc

Expression

Insect

Mutation

D435A/E437A, part of the DEDD motif; mutations p…

Promoter

Actin5C

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO-BcRF1-2xStrep

Plasmid

#138436

Purpose

Expresses full-length BcRF1 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1 (BcRF1 EBV (HHV-4))

Use

Tags

Strep

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-UL87-2xStrep

Plasmid

#138434

Purpose

Expresses full-length UL87 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

UL87 (UL87 HCMV (HHV-5))

Use

Tags

Strep

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-C-intein-C-spC9-H840A-N863A-2xNLS-hGH

Plasmid

#112211

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

C-terminus of dCas9

Use

AAV

Tags

Expression

Mutation

D10A

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-FDIO-SYP-HRP

Plasmid

#117187

Purpose

Plasmid name in publication: pAAV-FDIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

SYP-HRP

Use

AAV; Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL-c2X-ORF24-NTD

Plasmid

#138465

Purpose

Expresses MBP-tagged ORF24-NTD in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

MBP

Expression

Bacterial

Mutation

Amino acids 1-201

Promoter

tac

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-ORF24-NTD

Plasmid

#138464

Purpose

Expresses GST-tagged ORF24-NTD in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

GST

Expression

Bacterial

Mutation

Amino acids 1-201

Promoter

tac

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF24-3xFLAG

Plasmid

#138423

Purpose

Expresses full-length ORF24 with a C-terminal 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-EKAR

Plasmid

#181858

Purpose

Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-EKAR

Use

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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360 pCR kdrb

Plasmid

#13067

Depositor

Nathan Lawson

Article

Covassin et al Proc Natl Acad Sci U S A. 2006 Apr 25. 1

Insert

kdrb (kdr Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
p6H-SUMO3-ORF24-NTD-Strep

Plasmid

#138467

Purpose

Expresses SUMO3-tagged ORF24-NTD-Strep in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

SUMO3 and Strep-Tag II

Expression

Bacterial

Mutation

Amino acids 2-201

Promoter

T7

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTXB1-TP1

Plasmid

#160084

Purpose

Bacterial expression of Tn5-APEX2 fusion protein, no linker

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase/APEX2 peroxidase fusion protein

Use

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTXB1-TP2

Plasmid

#160085

Purpose

Bacterial expression of Tn5-APEX2 fusion protein with linker PAPAP

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase/APEX2 peroxidase fusion protein

Use

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Sox15

Plasmid

#128831

Purpose

dox-dependent expression of mouse Sox15

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Sox15 (Sox15 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pWHI2-NAT

Plasmid

#80588

Purpose

CEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoter

Depositor

John McCusker

Article

Vijayraghavan et al Genetics. 2018 Nov 29. pii: genetics.118

Insert

WHI2 (WHI2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Native WHI2 promoter

Available since

July 28, 2016

Availability

Academic Institutions and Nonprofits only