We narrowed to 18,373 results for: Met

1. pRSFDuetG-DmDCP1_328-366 (MCSII)-WT_G

   Plasmid

   #146406

   Purpose

   Bacterial Expression of DmDCP1_328-366

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   DmDCP1_328-366 (Dcp1 Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSMART-Ala2

   Plasmid

   #71326

   Purpose

   NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

   Depositor

   Michael Lynch

   Article

   Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

   Insert

   NADPH Alanine Dehydrogenase (ald Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D196A, L197R

   Promoter

   insulated waaHp from E. coli

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPPC018.AAV

   Plasmid

   #171146

   Purpose

   Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   hAAVS1 scRNA
   J1-BBa_J23117-mRFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 and J1-BBa_J23117

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPPC030.306

   Plasmid

   #171151

   Purpose

   Mevalonate production pathway with J306 scRNA on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   J306 scRNA
   J3-BBa_J23117-mvaES
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 and J3-BBa_J23117

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AlkB-D135G

   Plasmid

   #160434

   Purpose

   A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly

   Depositor

   Tao Pan

   Article

   Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

   Insert

   E. coli AlkB
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Changed Aspartic acid 135 to Glycine; deleted the…

   Promoter

   T7

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p415 Glo3 R59K Δ2x+ve

   Plasmid

   #112655

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Glo3 R59K Δ2x+ve
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   R59K, mutations (R223E, K224E, K225E, K233E, K234…

   Promoter

   Met25

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p415 GFP-Glo3 Δ2x+ve

   Plasmid

   #112662

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   GFP- Glo3 Δ2x+ve
   

   Use

   Tags

   sfGFP

   Expression

   Yeast

   Mutation

   Mutations (R223E, K224E, K225E, K233E, K234E, K23…

   Promoter

   Met25

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTCW018

   Plasmid

   #83531

   Purpose

   Aromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpin

   Depositor

   Claudia Vickers

   Article

   Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

   Insert

   pARO9-mf_2-pFUS1J2-ARO7i-CYC1t
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pARO9/pFUS1J2

   Available Since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas59

   Plasmid

   #82396

   Purpose

   sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas57

    Plasmid

    #82395

    Purpose

    sgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas56

    Plasmid

    #82394

    Purpose

    sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas55

    Plasmid

    #82393

    Purpose

    sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas52

    Plasmid

    #82392

    Purpose

    sgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xMma PylT_GFP 150TAG 6xHis

    Plasmid

    #140015

    Purpose

    Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available Since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-EGFP-C1-HsEDC3

    Plasmid

    #25032

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    EDC3 (EDC3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJH3717

    Plasmid

    #191477

    Purpose

    Pacr-2s-Arch::wCherry unc-54 3' UTR C.elegans A/B MN expression of Arch RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    Arch (arch-1 Nematode)
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pacr-2s

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28 His6-mEGFP-D4H

    Plasmid

    #226373

    Purpose

    Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

    Depositor

    Catherine Tomasetto

    Article

    Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

    Insert

    D4H (pfoA Clostridium perfringens)
    

    Use

    Tags

    His6 and monomeric EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMVtight-UPRT-T2A-RFP-IRES-CD

    Plasmid

    #126677

    Purpose

    Doxycycline inducible expression of UPRT and CD along with reporter RFP

    Depositor

    Joan Massague

    Article

    Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

    Insert

    UPRT-T2A-RFP-IRES-CD (FCY1 Budding Yeast, Toxoplasma gondii)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    tight TRE

    Available Since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEvol-MjaYRS

    Plasmid

    #153557

    Purpose

    Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli

    Depositor

    Huiwang Ai

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038

    Inserts

    MJaYRS (first copy)
    MJaYRS (second copy)
    amber suppression tRNA under proK promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    glnS and pBAD

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-APOBEC1-YTHmut

    Plasmid

    #131637

    Purpose

    Expresses APOBEC1 fused to a mutant YTH domain with diminished m6A binding

    Depositor

    Kate Meyer

    Article

    Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

    Insert

    APOBEC1-YTHmut-HA (YTHDF2 Rat, Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    YTH domain lacks AA 385-409 comprising the m6A-bi…

    Promoter

    CMV

    Available Since

    Oct. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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