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Plasmid#119860Purposeprecusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 CysteineDepositorInsertouter membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
UseTagsnoneExpressionBacterialMutationCysterine 290 to Serine and Glycine 244 to Cystei…PromoterlacAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_YFP
Plasmid#45295DepositorInsertYFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
Salsa-BC6-P1A6
Plasmid#202847PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC6DepositorInsertEGFP/BC6
UseTagsExpressionMammalianMutationPromoterTet Response Element (TRE)Available sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
VYAIFIFQL_epitope
Plasmid#165018PurposeExpression of CMV US20 (29-37) fused to IL-2 signal sequenceDepositorInsertCMV US20 (29-37)
UseLentiviralTagshuman IL2 signal sequence at N-termExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-Foxd3 in situ
Plasmid#37098DepositorInsertFoxd3 (Foxd3 Mouse)
UseIn situ hybridization probeTagsExpressionMutationPromoterAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAL725
Plasmid#119862Purposeprecusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287CDepositorInsertD-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
UseTagsnoneExpressionBacterialMutationLeucine 244 to Cysteine, Aspartate 287 to CysteinePromoterT5Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDZ645 pKAN 1x-mCherry-12xPP7 V4
Plasmid#73173PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interestDepositorInsert1x-mCherry
UseTagsExpressionMammalian and YeastMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRL1124
Plasmid#70690Purposeprovides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.DepositorInsertsavaiM
eco47iiM
ecot22iM
UseBacterial shuttle vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAGM1761
Plasmid#105381PurposeSynthetic biologyDepositorInsertpromoter AtDMC1 (At3G22880; 3000bp)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_AtPHYB-S_X
Plasmid#131183PurposeExpresses AtPHYB-S (Arabidopsis thaliana) followed by restriction sitesDepositorInsertAtPHYB-S
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_NcVVD-LOV_X
Plasmid#131176PurposeExpresses NcVVD-LOV (Neurosporra crassa) followed by restriction sitesDepositorInsertNcVVD-LOV
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
TTVTFLPTPSLILQT_epitope
Plasmid#165021PurposeExpression of CMV UL44 (38-52) fused to CD74DepositorInsertCMV UL44 (38-52)
UseLentiviralTagsCD74 at N-term; cathepsin S cleavage site (GRWHTV…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_ScPH1-S_X
Plasmid#131184PurposeExpresses ScPH1-S (Synechosystis PCC6803) followed by restriction sitesDepositorInsertScPH1-S
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_RsLP-LOV_X
Plasmid#131177PurposeExpresses RsLP-LOV (Rhodobacter sphaeroides) followed by restriction sitesDepositorInsertRsLP-LOV
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOG331
Plasmid#105408PurposeSynthetic biologyDepositorInsert6xHA tag fused to Twin-Strep tag
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-BsmBI
Plasmid#162801PurposeSTELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expressionDepositorInsertUb-BsmBI
UsePiggybacTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTEI071
Plasmid#105376PurposeSynthetic biologyDepositorInsertpromotor CAB3 (At1g29910; 1000bp)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a-TAX-L10-STEPtag
Plasmid#159375PurposeExpresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.DepositorInsertTAX-L10-STEPtag
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11a-STEPtag-L10-TAX
Plasmid#159374PurposeExpresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.DepositorInsertSTEPtag-L10-TAX
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCK032
Plasmid#105412PurposeSynthetic biologyDepositorInsertHormone-binding domain (glucocorticoid receptor)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D570A
Plasmid#129673PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b
UseCRISPR; Gateway compatible aaccas12b-d570a entry …TagsExpressionPlantMutationD570A; AacCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_VfAU1-LOV
Plasmid#131185PurposeExpresses VfAU1-LOV domain (Vaucheria frigida) preceded by restriction sitesDepositorInsertVfAU1-LOV
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_VfAU1-LOV_X
Plasmid#131174PurposeExpresses VfAU1-LOV domain (Vaucheria frigida) followed by restriction sitesDepositorInsertVfAU1-LOV
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_MxCarH-CBD
Plasmid#131189PurposeExpresses MxCarH-CBD (Myxococcus xanthus) preceded by restriction sitesDepositorInsertMxCarH-CBD
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_TtCarH-CBD
Plasmid#131190PurposeExpresses TtCarH-CBD (Thermus termophilus) preceded by restriction sitesDepositorInsertTtCarH-CBD
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 L717C C723S C724V (NT531)
Plasmid#50896PurposeExpresses human NKCC1 L717C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFPExpressionMammalianMutationL717C C723S C724V in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJOG301
Plasmid#105358PurposeSynthetic biologyDepositorInsertpromotor DD45/EC1.2 (At2g21740;1000bp)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG658
Plasmid#105409PurposeSynthetic biologyDepositorInsert3xHA tag fused to Luciferase CDS
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG298
Plasmid#105361PurposeSynthetic biologyDepositorInsertpromotor ICU2 (At5g67100;600bp)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG642
Plasmid#105406PurposeSynthetic biologyDepositorInsert3xHA tag fused to tagRFP-T CDS
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only