We narrowed to 7,484 results for: PAC
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Plasmid#127002PurposeBasic Part P- Promoter (non-constitutive); T7 promoter; recognized by T7 polymeraseDepositorInsertPart T7 promoter_P
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP19-Ptet_P
Plasmid#127005PurposeBasic Part P- Promoter (non-constitutive); Ptet; induced by tetracycline/analogue; controlled by TetRDepositorInsertPart Ptet_P
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHB-3
Plasmid#140956PurposeFor PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.DepositorInsertphaCAB
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHB-4
Plasmid#140957PurposeFor PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.DepositorInsertphaCAB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-puro-2A-TEVp
Plasmid#99610PurposeLentivirus expressing puromycin resistance gene and TEV proteaseDepositorInsertsPuromycin resistance (PAC gene)
TEV protease
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS1021-Tier1-PhCMV-PuroR
Plasmid#169492PurposeTier-1 vector encoding PhCMV-driven marker for puromycin selection (PhCMV-PuroR-pA)DepositorInsertPCMV-driven puromycin N-acetyl-transferase
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2
Plasmid#52961PurposeReplaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS-NSAvailable sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
doraWT_rescue_construct
Plasmid#190609PurposePuromycin-selectable expression of Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationPromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHB-2
Plasmid#140955PurposeFor PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.DepositorInsertphaCAB
UseTagsExpressionBacterialMutationR238S mutation in PhaCPromoterAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tmem98-mCherry
Plasmid#124505PurposeExpresses mCherry-tagged mouse TMEM98 in mammalian cells (C-terminal tag)DepositorInsertTmem98 (Tmem98 Mouse)
UseTagsmCherryExpressionMammalianMutationstop codon was removed to allow fusion with sGFP2…PromoterCMVAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUAS-Ncad-GFP
Plasmid#35986DepositorInsertNcad (cdh2 Zebrafish)
UseGal4 inducibleTagsEGFPExpressionMutationPromoter14xUAS-E1bAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1- Ncad-GFP
Plasmid#35676DepositorInsertNcad (cdh2 Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
Tmem98-sGFP2
Plasmid#124503PurposeExpresses sGFP2-tagged mouse TMEM98 in mammalian cells (C-terminal tag)DepositorInsertTmem98 (Tmem98 Mouse)
UseTagssGFP2ExpressionMammalianMutationstop codon was removed to allow fusion with sGFP2…PromoterCMVAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tmem98-mTq2
Plasmid#124504PurposeExpresses mTurquoise2-tagged mouse TMEM98 in mammalian cells (C-terminal tag)DepositorInsertTmem98 (Tmem98 Mouse)
UseTagsmTurquoise2ExpressionMammalianMutationstop codon was removed to allow fusion with sGFP2…PromoterCMVAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
PSMG1_pCSdest
Plasmid#53884DepositorInsertPSMG1 (PSMG1 Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
HA-doraWT_rescue_construct
Plasmid#190608PurposePuromycin-selectable expression of HA-tagged Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationPromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro
Plasmid#52963PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.DepositorHas ServiceCloning Grade DNAInsertsS. pyogenes sgRNA cassette
Puromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEf1-a and hU6Available sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
dora[B]_rescue_construct
Plasmid#190637PurposePuromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
dora[A]_rescue_construct
Plasmid#190639PurposePuromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
KA1270_pPB-gPou5f1_3.5kb-UBCmin-IP
Plasmid#124181PurposepiggyBac-based reporter (Puro) driven by the Pou5f1 enhancerDepositorInsertIRES-Pac, Pou5f1 enhancer
UseReporterTagsExpressionMutationPromoterUBCminAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP
Plasmid#124180PurposepiggyBac-based reporter (EGFP) driven by the Pou5f1 enhancerDepositorInsertEGFP-IRES-Pac, Pou5f1 enhancer
UseReporterTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-PmNG
Plasmid#232200PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreenDepositorInsertmNeongreen
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-PmNG
Plasmid#232201PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTyDepositorInsertmNeongreen
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-PmSc
Plasmid#232549PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-PAC-3xTyDepositorInsertmScarlet
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEA01
Plasmid#170766PurposeUtilisation of pSAM2 excision tool in Amycolatopsis sppDepositorInsertsxis, int
oriT
pac
UseTagsExpressionBacterialMutationPromotertrcpAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
attB53_SNCB+_attB53
Plasmid#183614PurposeRMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.DepositorInsertsLaG17 GFP nanobody SynNotch tTA
TRE-mCherry-SV40pA
tagBFP
UseRmceTagsMyc and human CD8a signal sequenceExpressionMammalianMutationPromoterAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-S-PURO
Plasmid#58244PurposeGATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven Puromycin resistanceDepositorInsertsattR1-Cm(R)-CcdB-attR2
Puro(R)
UseLentiviral and RNAi; Gateway destination vectorTagsExpressionMammalianMutationPromoterSFFV and noAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-PTy
Plasmid#232203PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: 3xTy-T2A-PAC-3xTyDepositorInsert3xTy
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-PTy
Plasmid#232202PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTyDepositorInsert3xTy
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-PmSc
Plasmid#232548PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mScarletDepositorInsertmScarlet
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[B]_rescue_construct
Plasmid#190638PurposePuromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[A]_rescue_construct
Plasmid#190640PurposePuromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
attB53_SNCB-_attB53
Plasmid#183615PurposeRMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.DepositorInsertsLaG17 GFP nanobody SynNotch tTA
TRE-mCherry-SV40pA
tagBFP
UseRmceTagsMyc and human CD8a signal sequenceExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBOB-EF1-FastFUCCI-Puro
Plasmid#86849Purpose3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selectionDepositorInsertsmKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1 and PGKAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
ROSA26(MultiFPsΔPuro)
Plasmid#140759PurposeTargeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).DepositorInsertspuromycin-N-acetyltransferase
Venus
mCherry
mCerulean
UseMouse TargetingTagsExpressionMutationPromoterCAGGSAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas9_nonPuro_OSS
Plasmid#186652PurposeCas9 plasmid to express the Cas9 in OSS cellsDepositorInsertOSS Cas9 Expression Plasmid
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-RanV19 E1RA2
Plasmid#118317PurposeExpression of human RanDepositorUseTagsGFPExpressionMammalianMutationin-frame insertion of RanV19 into GFPEpac1RA2 and…PromoterAvailable sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE4
Plasmid#181846PurposeFourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.DepositorInsertICUE4 (RAPGEF3 Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationGlutamine 122 in Epac1 domain mutated to glutamat…PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR
Plasmid#171594PurposeStrong human ACE2 abundance from Bxb1 landing padDepositorInsertsUseSynthetic Biology; Promoterless bxb1 dna recombin…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-dCas9
Plasmid#112233PurposeLentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2DepositorInsertsdCas9
Porumycin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationChanged: Aspartic acid 10 to Glycine, Histidine 8…PromoterEFS-NSAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only