We narrowed to 15,962 results for: GRN

1. 53BP1 N-terminal sgRNA

   Plasmid

   #207091

   Purpose

   pX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   GGGGAGCAGATGGACCCTAC
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc-y1sgRNA-Cas9

   Plasmid

   #49331

   Purpose

   Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

   Depositor

   Ji-Long Liu

   Article

   Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

   Inserts

   Cas9
   dU6-sgRNA
   y1 sgRNA (y Fly)
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Insect

   Mutation

   Human codon optimised

   Promoter

   Actin-5c and Drosophila U6

   Available Since

   Dec. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. PIK3C2B gRNA (BRDN0001145952)

   Plasmid

   #76714

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PIK3C2B (PIK3C2B Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRIM24 gRNA (BRDN0001145637)

   Plasmid

   #77887

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM24

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM24 (TRIM24 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. RPS6KA1 gRNA (BRDN0001145974)

   Plasmid

   #75497

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RPS6KA1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RPS6KA1 (RPS6KA1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. SP6-sgRNA-scaffold

   Plasmid

   #47912

   Purpose

   Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection

   Depositor

   Paul Sternberg

   Article

   Chiu et al Genetics. 2013 Aug 26.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   sgRNA 3' end

   Expression

   Worm

   Promoter

   SP6

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. SMC6 G4.4 gRNA

   Plasmid

   #90899

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SMC6

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   SMC6 (Guide Designation G4.4)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. SMC6 G3.4 gRNA

   Plasmid

   #90898

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SMC6

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   SMC6 (Guide Designation G3.4)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. JAK1 gRNA (BRDN0001149081)

   Plasmid

   #76394

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human JAK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   JAK1 (JAK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFREE2-SpCas9-sgRNA

    Plasmid

    #179582

    Purpose

    Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    Cas9
    

    Expression

    Bacterial

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. TK1 gRNA (BRDN0001145069)

    Plasmid

    #76424

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TK1 (TK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBluescriptSKII+ U6-sgRNA(F+E) TFRC

    Plasmid

    #74709

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

13. lentiCRISPRv2-TTF-1-gRNA

    Plasmid

    #101857

    Purpose

    Introducing a gRNA targeting human TTF-1 (NKX2-1) into cells

    Depositor

    David Mu

    Article

    Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

    Insert

    gRNA targeting TTF-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. PDGFRA gRNA (BRDN0001148373)

    Plasmid

    #75493

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PDGFRA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PDGFRA (PDGFRA Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. MAPK14 gRNA (BRDN0001145809)

    Plasmid

    #77923

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MAPK14

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MAPK14 (MAPK14 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pShoCAST-sgRNA_entry (BO1)

    Plasmid

    #181786

    Purpose

    Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    

    Expression

    Bacterial

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pShoHELIX-sgRNA_entry (BO3)

    Plasmid

    #181784

    Purpose

    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. RPS6KA3 gRNA (BRDN0001147852)

    Plasmid

    #75944

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA3 (RPS6KA3 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RPS6KA1 gRNA (BRDN0001148103)

    Plasmid

    #75498

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA1 (RPS6KA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. zCREST3:Cas9green; EGF_gRNA

    Plasmid

    #199333

    Purpose

    pDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    zCREST3
    Cas9
    nrg1 EGF sgRNA
    

    Use

    Tol2 destination vector

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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